LOCUS BG068494 585 bp mRNA linear EST 12-MAY-2010 DEFINITION H3066B09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3066B09 3', mRNA sequence. ACCESSION BG068494 VERSION BG068494.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 585) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068494.1. Other_ESTs: H3066B09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3066 row: B column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..585 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3066B09-3" /db_xref="taxon:10090" /clone="H3066B09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 119 a 129 c 129 g 208 t ORIGIN 1 gacccctttt aggactagtg agattcagtt tcatggttgg agaacttaat ggcaaagatt 61 tgtagcttgt tgacatggaa tttacttcac catcaaggcg tgtctgagtc ttggaagtgg 121 tggtaactgc cttttcctct tgagaactga caactgtgga gcacctcttg tcactggcgg 181 gattcacatc cacagggaaa ctgaactctt ggctcttccc accactgctg ctctcactgt 241 tgcagtctgt gctatctatg ttatccgagt cactatttcc acctgcgccg ccacctctag 301 gctctccatt tattttattt ttatctgctt tttgttgtgc taaagatata tgtggctcag 361 gtaaaattaa atgcgctgtg ggaggggact cttttgtctt attcttctta tttttccgat 421 tggtgctggg ggttgtcact acattggctc tttttttgcc aaatacattt gtaaacgtgg 481 tagatgtagc agagattcca attgtagtag tggtggttgc actcggaggc tctatgggaa 541 tttcttgctc cacatcttca tcattctctt catcttcacc ctctg //