LOCUS BG067951 716 bp mRNA linear EST 12-MAY-2010 DEFINITION H3060A10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3060A10 3', mRNA sequence. ACCESSION BG067951 VERSION BG067951.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 716) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067951.1. Other_ESTs: H3060A10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3060 row: A column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..716 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3060A10-3" /db_xref="taxon:10090" /clone="H3060A10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 170 a 182 c 207 g 157 t ORIGIN 1 aagggagaga tttttgtttt attaatgtat aaaccatatc ttttggggaa gagaagtggg 61 tactagggat tcaagactaa acaagcacat atcatagagt tggggcagct aaaacacaac 121 agggataatc gctgtgcatt ccccagggct gtgtggtgcg aactgtctat tcaccagagg 181 gtgtggccaa ggaggacaag actgtgcaca cacacagaga cagcagcctc ctctggggct 241 gagatgggca aggcctctca gatggtcaca ctgttttcca ccaggctggg tcggaggtac 301 tcatagggca ggtccaggct tgcattccgg acctcaatct ccttgtccat gatagccagc 361 tcctcctgga attgcttcag cacatcccgg ggcctggggc ctgagaaata ctcctcttta 421 tgctgcccca gggccaccat gacaggctgg cgtctgccaa ggaacttggt gactgttatc 481 tgcatccgtg cctgctgaag gcaaggcaac gagtccacaa tgtccctctc tgtcacatcc 541 ttggagatgg gtgggggctt ccgcatggtg catgggccat ttgggatcca ggcataccag 601 tccagctgac ccagatgtgt agatgcgtgc tgaccagtgc acgtgaagat gcacatagcg 661 acaaagcggc acagctcagc ccgggactct aaggacaggg ggaacctcgg tcctgg //