LOCUS BG067567 630 bp mRNA linear EST 12-MAY-2010 DEFINITION H3055G10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3055G10 3', mRNA sequence. ACCESSION BG067567 VERSION BG067567.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 630) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067567.1. Other_ESTs: H3055G10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3055 row: G column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..630 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3055G10-3" /db_xref="taxon:10090" /clone="H3055G10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 213 a 131 c 112 g 174 t ORIGIN 1 aaccagcaac attatttttt attctttctt gtttgctcag tgtttatttt ccttcaggtt 61 gtgagacaaa agtgtcacca agataattta aagtagataa tttaatgtca actctgccac 121 ctcaaaaatt gcacaaggaa cccccccaaa atcatgcaaa caatattcat caccaatcat 181 cctgaaacca gactcattaa agaaaatgga gagagtgaag aaaaggagaa agttagagaa 241 aactgttatg tacccaaatt ctgttgctac ccccagacct tggctgaata agtgcatcca 301 tttggtacag aagagccagt gcacaggtct gaactgctaa ccttgggagg gtctttaaag 361 agattagcac ttaattgctg tctggcaccc tttgctatag taagttacag tcctcataga 421 aaaatcactg accctgaagc tggattaggg atgttggcac acatatatga tcttgactac 481 aatgaccagt ccagctcact aacattccaa tctaataacc atttgcctaa aatctgactg 541 atggagtcag aaatgttttg gctggaagtg accatactct taaacaagaa acgaaacaaa 601 taggaaatca tcttttttcc tcaaagagct //