LOCUS BG067334 766 bp mRNA linear EST 12-MAY-2010 DEFINITION H3053C03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3053C03 3', mRNA sequence. ACCESSION BG067334 VERSION BG067334.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 766) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067334.1. Other_ESTs: H3053C03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3053 row: C column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..766 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3053C03-3" /db_xref="taxon:10090" /clone="H3053C03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 172 a 201 c 185 g 208 t ORIGIN 1 actgtctgtg gatccagctg gatctgcccc ttgcccatga tcttgtccac aatctcattg 61 ttgtctttgt tgaccttggt cttgtcgtag cacttctcat aacacaggat cctcagggac 121 tgtgaaccct ccagctcaat ctcaaactcc tcatcccact tgggctctgt cgtgtcccga 181 aacaccctgg tcttggcttt gctgacaaaa tagccaaagg aatccacctc cagagtacag 241 taaagattgg ctgactgttt aaagcccttg gcagagtgga cgatgacatg aaggaagccg 301 tacagtcctg gagactcatc atcatcttta ttgctggtga caggaatgtt gtgcacagtc 361 ctaagcttga aacaggatcc cgtgagcacc tggagctcca cagagctcag gacaaaggcc 421 tggagatcct tcttctgtag cttctgaatc gcttctctcc actccgacct ctcatagtca 481 gaagacagaa gaaacaggta actctttcca ttccgattgt gtatccggaa agggatcgtt 541 ggggaattga gcagcagtaa gaactcattc tcaaacatct tctttttgag gcgctcaatg 601 gctcggctct gtcctttgtt ggctttctcc ttctggatct cactcttgag ggcagagatc 661 ttcgttttca tgtcctcgag ctcgtggtct gggaaggggt gcacctgggg actggcctct 721 gactcctcag gggatgggac actaagtcag ccagagggat gtacac //