LOCUS BG065488 629 bp mRNA linear EST 12-MAY-2010 DEFINITION H3031G01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3031G01 3', mRNA sequence. ACCESSION BG065488 VERSION BG065488.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 629) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG065488.1. Other_ESTs: H3031G01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3031 row: G column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..629 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3031G01-3" /db_xref="taxon:10090" /clone="H3031G01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 187 a 141 c 123 g 178 t ORIGIN 1 aaacacaaag tttatgttaa cattaaagat ggaattttaa aaaaagcaaa ttcaagccct 61 taagttagta atattttcag gttagctgta taaaaaatgt actattttac atctccccat 121 actgcatact ataactgtac atacattttt cattccaaag tactcgtcac ttataaagtg 181 agagaattca ggctgcaaag taaacgtaac ttttaaacag ggctcccgtg aaagattgga 241 gcagcagtgc acatgagtct gcactcggga cttatatgga agacatctct cccttgtgct 301 tataactgat ctgcaaacag tcagtatttc tggaacttgt tctggagaaa atcacagcag 361 acaggggctc agggcagaca taagcagctc cattttataa acgagtttcg gccttccatc 421 ttgttccact gaacctcctt gaatggtcct cggagatgac tccacttgct gtcttgtaaa 481 acatcactct tcggaaggtc tttgcactgg ttacggggaa actgaaccat aatcttgctg 541 ccactctcag gtccgttacg aactgaaatc gcatagtctc cacttggcga ggccacggtg 601 atggccgaag cattgccaat gccctctat //