LOCUS BG064544 556 bp mRNA linear EST 26-JAN-2001 DEFINITION H3021A03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3021A03 3', mRNA sequence. ACCESSION BG064544 VERSION BG064544.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 556) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3021A03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3021 row: A column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..556 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3021A03-3" /db_xref="taxon:10090" /clone="H3021A03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 189 a 109 c 84 g 174 t ORIGIN 1 aagatttatt tattattata tgtaagtaca catagctgtc tttagacagt tcagaagagg 61 gcgtcagatg tcgttacaga tggttgtaag ccaccatgtg gttgctggga tttgaactca 121 ggaccttcaa ggaaggtctt caagtgctct taaccactga gccatattca attcccagca 181 cctacacaat agctcacaac tgcctgtaac ttcagttcca gggggtcttg ataccctctt 241 ctgacctatg caggtgaaac tctcataaaa tatttattta atttaaaaaa tattaagtaa 301 caccaataca ataaaaaata ctgaacagct tttaatccct atgctaattt aaataattaa 361 ctgaaagtat tttaattagt agaagtgaaa aattaaccat tcaatagtaa ctaccttata 421 atacatgtgc ttaacaattt aaaagacaaa ccagcatctg ctctatctgc agcatcttct 481 tcgcaacaga aacttcacta tcttagaaat ctcatttaat agaagcaaag ccttcttgtg 541 gaagcaccgt tttttt //