LOCUS BG064103 742 bp mRNA linear EST 12-MAY-2010 DEFINITION H3015E06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3015E06 3', mRNA sequence. ACCESSION BG064103 VERSION BG064103.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 742) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064103.1. Other_ESTs: H3015E06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3015 row: E column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..742 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3015E06-3" /db_xref="taxon:10090" /clone="H3015E06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 145 a 201 c 190 g 206 t ORIGIN 1 gcaattcaag gcttttgtta tttgttttgt cggtaaaaac atcaaagatc tccagatgca 61 gccagaaagc atttcccagg gcagtttctg ggtttacctg tttgcgtgtc tgttaacata 121 ggaactcagt ccagggcagg ttaaattcac attaatgtct gacccttctg ccctgtggtt 181 ctagttttga gtctgaacag agagatgggg gcatttttcc agttatgccc tgacctcgag 241 agaatgtcct ggtaggagtg cccctccact taggccccat agccttggct caatgtccaa 301 gcctcaggcc tggctctttt catcctgatc taggctggtc tcaacattca gaagctgctg 361 gcatgctggc ctcctggtgt cgtgtagtct gtgtgaaaag agcacttctg tcttctcctc 421 cactctgtct ctgtacgcac acgtttctcc agcctcacat cagccataac cagcatgctg 481 tggcgctgaa gtcatgccag ccttggctcc tccttgtcgt gggctgtggg ctccaagggc 541 aggcagggcc tggctgtcta gctaagtccc ccagtctaag ctggttctgt caggagacac 601 cccctccccc catccctaag cactagtgtc cacaatgcat ttgaggaaca ttgtgtcatc 661 tttgacgtag gcgtgcttgg gtgactgcag cttgctgagg gggaagaaga gcgggcagcg 721 ctggccacgt tggtctcact ct //