LOCUS BFAJ01000060 10826 bp DNA linear BCT 10-AUG-2023
DEFINITION Escherichia coli ME8077 DNA, ME8077-1_clc_PE_A1_ctg060.
ACCESSION BFAJ01000060 BFAJ01000000
VERSION BFAJ01000060.1
DBLINK BioProject:PRJDB6624
Sequence Read Archive:DRR120522
BioSample:SAMD00108506
KEYWORDS WGS; STANDARD_DRAFT.
SOURCE Escherichia coli
ORGANISM Escherichia coli
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
REFERENCE 1 (bases 1 to 10826)
AUTHORS Kobayashi,H. and Takaki,Y.
TITLE Direct Submission
JOURNAL Submitted (26-JAN-2018) to the DDBJ/EMBL/GenBank databases.
Contact:Hideki Kobayashi
Japan Agency for Marine-Earth Science and Technology, Research and
Development Center for Marine Biosciences; Natsushima 2-15,
Yokosuka, Kanagawa 237-0061, Japan
REFERENCE 2
AUTHORS Kobayashi,H.
TITLE Regeneration of Escherichia coli from Minicells through Lateral
Gene Transfer
JOURNAL J. Bacteriol. 200, e00630-17 (2018)
REMARK Publication Status: Online-Only
DOI:10.1128/JB.00630-17
COMMENT ##Genome-Assembly-Data-START##
Assembly Method :: CLC Assembler v. 10
Assembly Name :: ME8077-1_clc_PE_A1
Genome Coverage :: 115x
Sequencing Technology :: Miseq illumina
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..10826
/culture_collection="NBRP:ME8077"
/db_xref="taxon:562"
/mol_type="genomic DNA"
/organism="Escherichia coli"
/strain="ME8077"
/submitter_seqid="ME8077-1_clc_PE_A1_ctg060"
BASE COUNT 2210 a 3160 c 3332 g 2124 t
ORIGIN
1 atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt ggagtcatta
61 cccggtaaca gatatatcgc ctccatcacc gctgaagtaa tgaagtaagg cagcaagcct
121 cgccatgttc tccgccattt tagaagcata atccttaaaa tcataaagaa ggccaatcat
181 tctcatttct gactcgacct ggttgtagaa ttcgatccaa tgtctttctg cttctgcaga
241 gaatcggagg cagatacgat tattttcaca cacggacggg tttgcctcca cctttgtaag
301 aaagaattgt gaaatggggt tgcaaatgaa taattacaca tataaagtga attttaattc
361 aataagtggc gttcgccatg cgaggataaa atgtccgatt tatacaaaaa acactttctg
421 aaactgctcg actttacccc tgcacagttc acttctctgc tgacccttgc cgcacagctc
481 aaagccgata aaaaaaatgg caaggaagta cagaagctta ccggtaaaaa catcgcgctc
541 atcttcgaaa aagactcgac tcgtacccgt tgctctttcg aagttgccgc atttgaccag
601 ggcgcgcgcg ttacctattt agggccgagc ggcagccaga ttgggcataa agagtcaatt
661 aaggacaccg cgcgggttct cgggcggatg tatgacggca ttcagtatcg cggtcacggc
721 caggaagtgg tcgaaacgct ggcgcagtat gcgggcgtgc cggtgtggaa cgggctgacc
781 aacgagttcc acccgaccca gctgctggcg gacctgatga ccatgcagga gcacctgccg
841 ggcaaggcgt ttaacgagat gacgctggtc tacgcgggcg atgcgcgcaa caacatgggc
901 aactcgatgc tggaagcggc ggcgctgacc gggctggatc tgcgcctgtt ggccccgaaa
961 gcctgctggc cggaagagag cctggtggcg gagtgcagcg cgctggcgga gaagcacggc
1021 gggaaaatta ctctgacgga agacgtggcg gcaggcgtta agggcgcgga ctttatctat
1081 accgacgtgt gggtgtcgat gggcgaggcc aaagagaagt gggcagagcg gattgcgctg
1141 ctgcgcgggt atcaggtgaa cgcgcagatg atggcgctga ccgacaaccc gaacgtgaag
1201 ttcctgcact gtctgccggc gttccatgac gaccagacta cgctcggcaa gcagatggcg
1261 aaggagttcg atctgcacgg cgggatggag gtgacggacg aggtgtttga gtcggcggcg
1321 agcatcgtgt tcgaccaggc ggaaaaccgg atgcatacga ttaaggcggt gatgatggca
1381 acgcttgggg agtgattggg tccgctgcgc gttggtgccc tcaccccggc cctctcccac
1441 agggagaggg agaacaccgg ctccatttca ttgatttttc atcccgaaaa aggtacgttt
1501 tcgccttaat tccagcgtgg acatgccagc attatgccga ttattcagtc tgttgaacgt
1561 gcgttgcaga tcctcgacct gttcaacgag caggccaccg agctcaagat caccgacatc
1621 agcaaactga tggggctgag caagagtacc ctccactcgc tgctaaaaac cctgcagctt
1681 cacggctata tcgatcagaa cccggagaac ggcaagtatc gcctcggcat gaagctggtc
1741 gagcgcggcc attttgtcgt gggctccatc gatattcggc agaaggcaaa aggctggctg
1801 acggagctgt cccggcggac cgggcagacc acccatctgg ggatcctgga cgggcgtgaa
1861 ggggtctata tcgagaagat tgaaggcaag ctggccgcca tcgcctattc acgcatcggc
1921 cgccgcctgc cggtgcacgc caccgccatc ggcaaggtgt tgattgcctg gctgggcgag
1981 gccgagctga acgccctgct ggagggctat cagtacacta cctttacgcc cgccaccctc
2041 gcgtctcgcg aagccttaat gagcgccctg gcgcagaccc gcgagcaagg ctacgccctg
2101 gacagcgaag agaacgagca gggcgtgcgc tgcgtggcgg tgccggtgtg gaaccacgag
2161 tcccgcgtca tcgccgccct gagcctgtcg acgctgacct cccgcgtgga cgacgcggag
2221 ctggctaatt tccgcgagca gcttcagcag gccgggctcg cgctctcgcg cgcgctgggc
2281 tacccggcct gagccgttag accggctcgt aggtgaagta gtcgaagtcc gcgtagcagc
2341 cgtcgccgct gatgtcctcg cagtgcaggc ccacaaacgc gccggtgaag aagccgcgcc
2401 cgccgatgta gtcgtccgac agcttccacg cctcatacgt caccggcacg gtgtgccacg
2461 tctcgccatc aaacgagtag ctgtagcggt agaccagcgt atccacgtcc acccgcagcc
2521 agacgctctc cgcatgttcc ggcaccggaa tgggctgctc gtgcagcggc cacgacggca
2581 cgttgtggtc gagctggata actttgatgg ttctaccctg tccctcctcg tagtccacaa
2641 agcagtagct ccagtttttg ctgttgtagt agcaggtcag ccccgcgctc tgctggaagt
2701 gcaccggcga gaactccatc cgcgtttctg cccggaaggc gaagtgctgc cagcggcgcg
2761 ccacggtcga ttgggtgaag gtcgaattga gcgagtcgtt gccatagagc cgtaagaagc
2821 ccgggcgcgc ggtgagcgag ccgagggtgt cgtcgaacgg aatgcgcagg gtctgcagct
2881 ccgggtcaag cgaactggcg tcgaaatcgt cccgccagtt gcccggaacg gctgcaggct
2941 gctcggctac ttgcgggcct ttcacggtca gctgcgcgtg cttgccgcct tccacgtacg
3001 gccagccgtc gcgccattca atgcgggcga tgccggtctc gcgccccagc gggcagtagc
3061 cgcgtccgcc ggaggccagc agcggcacgc cgggcaggcg cagcgggcgg ctggtgaggt
3121 aggccatgta ccattcaccc gtatgcgtct gcagcagcga gccgtggccg ctcttctgca
3181 gcgggttctc cggcaggtgc cagctggtca tcatcgttac gtccgggtgc agctcgtacg
3241 gcccgtcgat atttttggaa cgcagcacca cgacggcgtg ctcgtagctg gtgccgccct
3301 cggcggccat caggtagtac catcccgcgt ggcgatacaa gtgcgcgcct tcggtgtagc
3361 agagcggcgt gccggtaaac agcgttttgc gctcgggcga gagcgtgccg gtctgcgggt
3421 caaacgcctg taacacgatg gtgttgtgcg ggttgctgtg gtggcgcggc ccccacgggc
3481 ggtagatata gtatttgcgg ccatcgtcgt cgtggaacag ggacgggtca aacccgccgt
3541 tgcccatcgg gattggctcg ctccatggcc cctcgatgga gggcgcggtg acgaggaagt
3601 tgcggccgtt tttccacggc gagtcgacaa tcttcacgtc ggtgtagagc agccagaatt
3661 taccgtcggc gtagctcagg cacggcgccc agatgccgcc ggagtccggg ttgcccttca
3721 tgtccagcat cgacacgcgg tccaacgggg tgctgaccag cgaccagttt ttcaggtcac
3781 gggagtggta gatgcgcacg cccgggaacc actcgaaggt cgaggtggcg atgtagtagt
3841 cctcgccctg gcggcacagg gacgggtccg ggttgaagcc ggtgagtatc gggttagtga
3901 tttccatatt gcctccggtt aatgggatgc ggctgtcgcg gcgtcgggaa cggcctcgcc
3961 ctgcgccgcg cggtgcttaa tcagctgccg gctgatggcc tccacgcggg catcggtgag
4021 cttgtagagc gacagcatga tgaacatgcc cgcgtagagc accaccggca ccacgcagaa
4081 cagaattttg atggtggtga gcacctcaac cggctgcacg ctgctgctgg cggaatagtt
4141 gacgtacgcc aggatccagc ccaccaccgc cccgccaatc gccaggccaa tcttcaggct
4201 gaacaggtag gtggagaaca ccagcccgtc gaggcggcga ccgctgcggc tctcctcgta
4261 gtccaccacg tcagaagcca tcagccactg cagcggcgtg gtggtattaa agacgaacag
4321 gaacaggatg ttgagggcaa aaatgagcgc gatgtgctcc gccggggtga cgaaaatcag
4381 caggctgatc agcgagtagg cgacgatgat ccacttgaag gcggtgacgc ggtcgaagcg
4441 gcccagcagg cgtgaggagc aaagcgagcc gaacatggtg gcgaggctgc cgtaaagtaa
4501 aaactgggtc gccaactccg ggtgatccat cacgtatttc acgaagtaga gcgtcgcccc
4561 gccgcgcacc acgttggagc aggtcgccat catcttgaac gcgcacatga tgcgccactg
4621 gctgttgccc agcagcagct taaggtcttt cgccaccgac gagcccggct gcacctcaaa
4681 ggtgtagcgc tctttggtcg tgaagaagca gacgtagagc agcaccacgc cgctcagccc
4741 cagcacgcac atggcgccga agtagcccac ctgctcgtcc cctttgccga tgatgctcac
4801 cagcggcagc gcgatgccgc tgatagcgag cgagcccgcc gccgccagga agaagcgcca
4861 ggactgcagg gcgtgacgct ctttcgggtc ggcggtgatg acgcccggca tggcgcagta
4921 cggcacgtta acgaaggtgt agaccagggt caggagaatg taggtcacgc aggcgtagat
4981 gatcttgccc tgtgcggaga agtccggcgt gtagaaggtc agcacgcaga cgatgccgaa
5041 cgggatggcc ccccacagca ggaacgggcg gaactggccg tgccgcgtgc gggtgcggtc
5101 taccagcagc cccatcagcg ggtcggtgac ggcgtcgagc acgcgggaga ccaaaaacag
5161 cgtgcccata atccccgccg acaggccgaa gacgtcggtg tagaaatagg ccagcaggaa
5221 catcgtggcc tgccagacga agccgcaggc ggtgtctccc agcccgtagc caattttgtc
5281 tttcatggtt aattgcgtca tcagaattac acccttatgt agtgtgccga attacaacat
5341 cgtgttaatg gagtgtaatg agctattcaa ttaaccagca atggtcatat cttatttccg
5401 aattacgtta ttttgctttt gtgacgccaa cgatatatct caactaattg tttaaattaa
5461 taaatttcaa aaacaacaaa gccgtctgcg ggaaatattc cgcagacggt tttatctctt
5521 aaaataatta aattccgagc gcttttttac cggcgttaat tacctcgata attttatcgg
5581 tgtcataaat acagcctttc caggtgccgc cgctcaccga ctgcagtgcc gcccacagcc
5641 gggtgtcgtc cggcaaaaag tcgtgggcgt gcaggtccgg gtgcgtctgc cgccgcgcca
5701 gctcgcgcgc gccctcttcc ggcgtcagcg ggttgtccgc ggtgccgatg aagttcacgc
5761 tgccagttaa cgtcagacga tccacggcaa tctcgatgat gtcgttatcg cgcagcttgc
5821 caatcggccc gcccgccagc gcctccggcg acacgtggcc gaagcaggcg cccgtcgaca
5881 cgcccgagaa gcgcgcatcg gtgatgagcg acaccgtctt gccccacgag atatgcttta
5941 gcgcggaggt gagctggtag gtctcttcca tgccggtgcc ggacggcccg ccgccgatca
6001 ccaccatgat atcgccctgc acaatctctt cccgcttgat cgccttgatc gcctgcgctt
6061 ccgagacaaa cacccgcacc cggccggtgt ggtggtatac gccatcttcg cccaccaccg
6121 acgggtcgat cgccgtggcc ttgatcaccg aaccttccgg cgcgatgttg cccgtcggga
6181 agcagaccgt cgaggtcagc cctttcgctt ttgccttctc cggcggcagg atcacgtcat
6241 ccggctctac gccgtcctgc tcgcgcaggc actggcggaa gcgcgcccgg cgctcggacg
6301 cctgccacca ttcaaggttc tcgcccaccg tctggccggt cacggtcatg gcgtccagat
6361 gcagcaggcc gaggtcgcgc aggtggagca tcacctccgg cacgccgccc gcgaggaagg
6421 cgcgcacggt cgggtgatag tccgggccgt tgggcagcac gctcaccaga cgcggcactt
6481 tacggttgat gcgcgtccag tgctcaacgt ccgggatcgt gcagcccgcc gcgtgggcga
6541 tggccggaat gtgcagcagt aaattggtgg agccgccgaa cgccgcgtgg atcaccatcg
6601 cgttttcgat ggctttatcg gagaggatat cccgcgtggt gatgccgcgg ctatccagct
6661 cgctgaccgc gcgcgccgac tggcgggcga tctccagcca caccgcctgc ccggacggcg
6721 ccagcgcgga gtgcggcagc gccagaccca gcgcctccgc gaccacctgc gaggtgcccg
6781 ccgtgccgag gaactgacac ccgccgcccg gcgaggcgca ggcgcgacag cccagttcgg
6841 cggcctcctg cagggagagt tcgtggttgg cgaaacgcgc gccgatggtc tgcaccttgc
6901 ccgcgtcttc cccgacggtc ggcggcagcg tcgccccgcc cggcaccaga atagtcggca
6961 ggtcgtgcat cgcggccagc gcaatcatgg tggcgggcag ccctttatcg caggtcgcta
7021 cgccgatcac cgcccgccgc gtcggcaggg agcggatcag gcggcgaaac acgatcgccg
7081 cgtcgttgcg gtacggcagg gaatcgaaca taccgtgcgt gccctgcgag cgcccgtcgc
7141 acggatcgct gacgaaggcc gcgaacggga tcccgccatt gcgggtgatc tccttcgccg
7201 ccgcctgcat ctgcatgccg atctcccaat gcccggtgtg gtagcccagc gcgattgggc
7261 gtccgtctcc ggcgcggatg ccgccctgag tgccgataat cagcacctct ttgccggtga
7321 gcttgttggc gtcccagccc atcccggcgt tctgcgtcat gccgaacagg ttgccgctgg
7381 gagattccat cagcatctgc ggggtcagcg gcagagcgcc ctgcggcccc gccgcgtggg
7441 tgatcaccgc ataaaacgcg tcgtcctgcg gggtgaaaat tttctcaatg gtcatcgtta
7501 tcgtccggct cagcaaagct tgagctgttg cagcagggtt ttcagctgcg ccttgcgcgg
7561 ctcgtccagc ggcgaggcgg gcggcagcac gtgcgtggag acaggacgac cgcagagcac
7621 gatcgcctct ttaatcacgt tcacaaacgg cgtatccagc tgatacatct gcggaatttg
7681 cagcaaggtc tgatgatacc cggccgcttt cgccacgtcc ccgtcgcgcc aggctttcag
7741 aagattcacc gacacctgcg gggcaaagtt gccgctcgcc gatatcgccc cgtcgccgcc
7801 gagcagcagg gtattgaaca gatgatcgtc gtagccgcag agcacggtga agtgcggatg
7861 ggcacctttg acggtatgga tcatgctgcg caggtgggcg acggagtcga tggtgtcttt
7921 gatgccgata atattgctgc gcgagtcggc gagggttttc accagcgccg gagtcagatc
7981 ctgcccggtc agcgccggga agttatagag catcaccggc agcgtgacgc tgtcggccac
8041 ctgctcgaaa tagcggatca ggttcgcttc cgacactttc cagtagtagg ggttgatcac
8101 cacgatgccg tccgcgcccg cctgctgcgc gtgctggctg agttcgatgg tttcccgggc
8161 gttggtgccg ccggtgccga tcagcaccgg cacgcgacga tcgacatgat cgatagcaaa
8221 gcgggcaatg gctttacgct cttcggcgcc gagctgggag aactcgccac cgctgcccag
8281 gaagaacagg ccgtcaacgc ctgctttgat cagatcgtcg atcagcgcgg cggtgcccgg
8341 cttatcgagc tggccgtcgg cggtaaaaat ggtggagaca gggggaatga ttcccgtgaa
8401 caacgcggac tgcggcatga gatctccttg ctgaatcatt ttgttctaca ttatagaaca
8461 gcgttcgttt atttaacgat catactatga cgcagaacaa ggagaggtaa atcggaaggg
8521 aagagggagt gcgggaaatt taagctggat cacatattgc ccctcacccc ggccctctcc
8581 ccaaaggggc gagggggaaa agatggctcg gtgcagtccc ctctcccctt tggggtgtac
8641 ggtccgggga catagtgaac acttgttcgg ggacatggta gacacttaca actaaggcat
8701 aagaacccgt ttatggagtc gcttatgccc tgggatgcga gagataccat gtcattacgt
8761 actgagtttg ttttgttcgc ctcgcaggac ggggcgaaca tccgttccct ctgccgtcgc
8821 ttcggcattt cacctgccac cggctacaag tggctccagc gctgggctca ggaaggtgcc
8881 gccggtcttc aggaccgccc gcgcattccg caccattccc cgaaccgctc atctgacgac
8941 atcacggccc tgctgcgtat ggcccatgac cgtcatgaac gctggggagc ccgcaagatt
9001 aagcgctggc tcgaggacca ggggcacacc atgcccgcct tcagcaccgt ccataacctg
9061 atggcccgcc atggcctgct gccgggcgct tcaccgggca ttcccgccac gggccggttc
9121 gaacacgacg cgccgaaccg cctctggcag atggatttta agggccactt tccttttggc
9181 ggtggacgct gccatccgct caccctgctg gacgaccact cccgtttttc cctgtgcctg
9241 gcgcactgta ccgatgaacg gcgcgagacc gtgcagcagc agctggtcag cgtgtttgag
9301 cgttacggcc tgccggaccg gatgaccatg gataacggct caccgtgggg cgacaccacc
9361 ggcacctgga cggcgctgga gctgtggctg atgcgcctgg gtatccgggt ggggcactcc
9421 cggccttatc atccgcagac gcaggggaag ctggagcgtt ttcaccgcag cctgaaggcg
9481 gaagtgctgc agggaaaatg gttcgcagac agcggtgaac tgcagcgcgc cttcgaccac
9541 tggcggacgg tctataacct tgaacgcccg cacgaggcgc tggatatggc ggtaccgggc
9601 tcgcggtatc agccgtcagc gcggcagtac agcggcaaca caacgccccc ggaatacgat
9661 gaaggggtga tggtcaggaa agtggatatc agcggaaagc tgagcgtgaa aggggtaagt
9721 ctgagcgcag gcaaggcgtt caggggagaa cgggtcgggc tgaaggagat gcaggaagac
9781 ggcagctacg aggtgtggtg gtacagcacg aaagtggggg tgatcgacct gaagaaaaag
9841 tcgatcacca tgggtaaagg atgttaaaaa gtgttcacca tgtccccgac cacctgattg
9901 tcggcggcag gcaaatattc tcattcgccg aacatggtct tctttaacag gaataaaatt
9961 atgaataaag actctactca aacttggggg cttaaacgag atatcacacc gtgtttcgga
10021 gcacgactgg tgcaggaagg tcatcgtctg cattttctgg ctgaccgggc tggattcacc
10081 ggctcattca gtgaggtgca gactctacaa ctggatgaag ccttcccgca ttttgtcgca
10141 catctggagc tgatgctgct ctcctgtgag ctgaatccca ggtacgcgca ctgtgtcaca
10201 ctgtaccgta acgggctgac tggtgaagcc gatacgctgg gcagtcatgg gtatgtatat
10261 atcgcaattt tgaatcgcca cgggtttaac agacacctcc gagtcattta aaatggctta
10321 aagagaggtg cccatgagcg gtaagcgtta tcccgaagag tttaaaactg aagcagtcaa
10381 acaggttgtt gatctcggtt attctgttgc cagcgttgca acacgtctcg atatcaccac
10441 ccacagcctt tatgcctgga taaagaagta cggtccggat tcttccacta ataaagaaca
10501 gtcagatgct caggccgaga tccgccgtct ccagaaagag ctgaaacggg ttaccgacga
10561 acgggacata ttaaaaaaag ctgctgtaga ttcaatctgt caatgcaaca cccctttcaa
10621 ttatctcttt cggtgttttg aacttcagtg tctttctcgg tctgttgttt agctgagcag
10681 caaccagatc tagttcatgt tgagtatatt gggcaagaca tgtcttttta ggaaagtact
10741 gccgaattag cccatttgtg ttctcggtga tgctgccaac ttactgattt agtgtatgat
10801 ggtgtttttg aggtgctcca gtggct
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