LOCUS BE516546 593 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE611_A02_B03ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE611_A02_B03, mRNA sequence. ACCESSION BE516546 VERSION BE516546.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 593) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..593 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE611_A02_B03" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 79 a 207 c 181 g 126 t ORIGIN 1 cgaattcgcg gccgcgtcga cctttattat ccttcctcct cctcctgctg ctgctgctgc 61 tggtcatggc ggtcatggac tcatggtggt gctggtgctg gtgcttcctc acaagcagcc 121 gcccccgtag ccggcgttct tgaacagctc ctgcttgatg gcgtccgcgc tgatcatctc 181 cctctgcatc tcggagcagg aggcgtgcat ggcgaagtcg ttgaagcagc tgacgacgca 241 ctgctggatg tcgaggccga gggtgtcgag cgtgcccacc gtcgacagca gcagccccgg 301 cttggccgcg cagtagacct ccacccgggt gtcgccctcc tccttgcgct ccacctcaac 361 tgggggtgtt ccgggcgagc atctcgttgg ggttgagctc ccggaacacg ctcagcagcg 421 gcgctgccgc cgccgccgcc ggcccgggat gctggtgcgg acacgcctcc tccatctcct 481 cctgcagccg ccggatcctc tccagcagct ccttcatgta gtcgatggtg tcccccagga 541 tcgacgtcct gtccatcttg ctgatcttgg gcaccacgga gcggagcatg gag //