LOCUS BC032999 3548 bp mRNA linear HUM 17-JUL-2006
DEFINITION Homo sapiens patatin-like phospholipase domain containing 8, mRNA
(cDNA clone MGC:26092 IMAGE:4829433), complete cds.
ACCESSION BC032999
VERSION BC032999.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3548)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3548)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (21-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC032999.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 34 Row: g Column: 5
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 48675815.
FEATURES Location/Qualifiers
source 1..3548
/db_xref="H-InvDB:HIT000041752"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:26092 IMAGE:4829433"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3548
/gene="PNPLA8"
/gene_synonym="IPLA2(GAMMA)"
/gene_synonym="IPLA2-2"
/gene_synonym="IPLA2G"
/db_xref="GeneID:50640"
/db_xref="HGNC:HGNC:28900"
CDS 408..2756
/gene="PNPLA8"
/gene_synonym="IPLA2(GAMMA)"
/gene_synonym="IPLA2-2"
/gene_synonym="IPLA2G"
/codon_start=1
/product="patatin-like phospholipase domain containing 8"
/protein_id="AAH32999.1"
/db_xref="GeneID:50640"
/db_xref="HGNC:HGNC:28900"
/translation="MSINLTVDIYIYLLSNARSVCGKQRSKQLYFLFSPKHYWRISHI
SLQRGFHTNIIRCKWTKSEAHSCSKHCYSPSNHGLHIGILKLSTSAPKGLTKVNICMS
RIKSTLNSVSKAVFGNQNEMISRLAQFKPSSQILRKVSDSGWLKQKNIKQAIKSLKKY
SDKSAEKSPFPEEKSHIIDKEEDIGKRSLFHYTSSITTKFGDSFYFLSNHINSYFKRK
EKMSQQKENEHFRDKSELEDKKVEEGKLRSPDPGILAYKPGSESVHTVDKPTSPSAIP
DVLQVSTKQSIANFLSRPTEGVQALVGGYIGGLVPKLKYDSKSQSEEQEEPAKTDQAV
SKDRNAEEKKRLSLQREKIIARVSIDNRTRALVQALRRTTDPKLCITRVEELTFHLLE
FPEGKGVAVKERIIPYLLRLRQIKDETLQAAVREILALIGYVDPVKGRGIRILSIDGG
GTRGVVALQTLRKLVELTQKPVHQLFDYICGVSTGAILAFMLGLFHMPLDECEELYRK
LGSDVFSQNVIVGTVKMSWSHAFYDSQTWENILKDRMGSALMIETARNPTCPKVAAVS
TIVNRGITPKAFVFRNYGHFPGINSHYLGGCQYKMWQAIRASSAAPGYFAEYALGNDL
HQDGGLLLNNPSALAMHECKCLWPDVPLECIVSLGTGRYESDVRNTVTYTSLKTKLSN
VINSATDTEEVHIMLDGLLPPDTYFRFNPVMCENIPLDESRNEKLDQLQLEGLKYIER
NEQKMKKVAKILSQEKTTLQKINDWIKLKTDMYEGLPFFSKL"
BASE COUNT 1182 a 600 c 720 g 1046 t
ORIGIN
1 acttccgctg ggggcggagc ggggcggggc tgagtgggtg gcgacctagc tgctgcgcca
61 gtgtttgtgt tggaagctca gctgatgcag gccggttgga gtggacgtca ttgccgggaa
121 cgagcgagtc gccgctgcag ccctagtgac tgcggcctgc atcccgattg tcttctcctc
181 caaggtctac atgattacct gaagtttaat aattttgcct ttctagagtg ttatacagct
241 ggaatcatac tgctatggtc cgaatgtttg tgcctctcaa aattcatgtg gaaatcataa
301 ccgctaaagt gatggtatta agaggtaaga ccatgaatta tggcatttct taaatgaagc
361 gttcaagaag tgagagaatg tcatagaaaa taaatgattt ttaagttatg tctattaatc
421 tgactgtaga tatatatatt tacctcctta gtaatgcaag aagtgtttgt gggaagcaga
481 gaagcaagca actgtatttc ttgttctcac ctaagcatta ctggaggata agccacatca
541 gtctacaaag aggttttcat acaaacataa taagatgtaa atggaccaaa agtgaagcac
601 attcttgcag taagcactgt tactctccaa gcaaccatgg tttacatatt gggattttga
661 aacttagcac ttctgctccc aagggactta caaaagtgaa catttgtatg tcccgtatta
721 aaagtacttt gaactctgtt tcaaaggctg tttttggcaa tcaaaatgaa atgatttcac
781 gtttagctca atttaagcca agttcccaaa ttttaagaaa agtatcggat agtggctggt
841 taaaacagaa aaacatcaaa caagccatca aatctctgaa aaaatatagt gacaaatcag
901 cagaaaagag tccttttcca gaagagaaaa gtcacattat agacaaagaa gaagatatag
961 gtaaacgcag tctttttcat tacacaagtt ctataaccac aaaatttgga gactcattct
1021 actttttatc aaatcatatt aattcatatt tcaaacgtaa ggaaaaaatg tctcaacaaa
1081 aggaaaatga acatttccgg gacaaatcag aacttgaaga taaaaaggta gaagagggga
1141 aattaagatc tccagatcct ggcatcctgg cttataagcc aggctcagaa tctgtacata
1201 cggtggacaa gcctacaagt ccttctgcga tacctgatgt tcttcaagtt tcaactaaac
1261 aaagtattgc taactttctt tctcgtccca cggaaggtgt acaagcttta gtaggtggtt
1321 atattggtgg acttgtcccc aaattaaagt atgattcaaa gagtcagtca gaagaacagg
1381 aagagcctgc taaaactgat caggctgtca gcaaagacag aaatgcagag gagaaaaagc
1441 gtttatctct tcagcgagaa aagattatcg caagggtgag tattgataac aggacccggg
1501 cattagttca ggcattaaga agaacaactg acccaaagct ctgcattact agggttgaag
1561 aactgacttt tcatcttcta gaatttcctg aaggaaaagg agtggctgtc aaggaaagaa
1621 ttattccata tttattacga ctgagacaaa ttaaggatga aactcttcag gctgcagtta
1681 gagaaatttt ggccctaatt ggctatgtgg atccagtgaa agggagagga atccgaattc
1741 tctcaattga tggtggagga acaaggggcg tggttgctct ccagacccta cgaaaattag
1801 ttgaacttac tcagaagcca gttcatcagc tctttgatta catttgtggt gtaagcacag
1861 gtgccatatt agctttcatg ttggggttgt ttcatatgcc cttggatgaa tgtgaggaac
1921 tttatcgaaa attaggatca gatgtatttt cacaaaatgt cattgttgga acagtaaaaa
1981 tgagttggag ccatgcattt tatgacagtc aaacatggga aaacattctt aaggatagga
2041 tgggatctgc actgatgatt gaaacagcaa gaaaccccac atgtcctaag gtagctgctg
2101 taagtaccat agtaaataga gggataacac ccaaagcttt tgtgttcaga aactatggtc
2161 attttcctgg aatcaactct cattatttgg gaggctgtca gtataaaatg tggcaggcca
2221 ttagagcctc atctgctgct ccaggctact ttgcagaata tgcattggga aatgatcttc
2281 atcaagatgg aggtttgctt ctgaataacc cttcggcatt agctatgcat gagtgtaaat
2341 gtctttggcc agatgtgccg ttagagtgca tagtatccct gggcactgga cgttatgaga
2401 gtgatgtgag aaacacggta acatacacaa gcttgaaaac taaactttct aatgttatca
2461 acagtgctac agatacagaa gaagtccata taatgcttga tggcctgtta cctcctgaca
2521 cctattttag attcaatcct gtaatgtgtg aaaacatacc tctagatgaa agtcgaaatg
2581 aaaagctgga tcagctgcag ttggaagggt tgaaatacat agaaagaaat gaacaaaaaa
2641 tgaaaaaagt tgcaaaaata ttaagtcaag aaaaaacaac tctgcagaaa attaatgatt
2701 ggataaaatt aaaaactgat atgtatgaag gacttccatt cttttcaaaa ttgtgatgag
2761 tatatgctta tgttctcata aatgaaggtc tgtttagaag atcaaccaca ttcaataagg
2821 aattgtgggg ttcgacatga gttaactttg aaatacgtat gaattctgga gaatcctgaa
2881 aaagacggtg cttcaaccag cttgcatagc acagagaata ttcttggtta cagaattcat
2941 atgggaacta ggcttttaag atgttaataa ttagctaagc tttagtaacc cttactgtgc
3001 tagtagattt tagtagatat tggtgttata ttgtttgatg tttgaaaata tattaatata
3061 tgtgccgaac aagaaaccga aagctatatt gtactgtgta tttttacttt agtcctcata
3121 atcatgttga atttatgtga tcattgattt tatttcatat ggaaaagcta atttcttctt
3181 aaatttacat tacctaatat tctcactagc tatgttctcc aatccacact gccttttatt
3241 gtaatatcat ctaaatagat gcagaaaaat ggaattttct ctattaaagt attttacatt
3301 tgacataaaa aagaaccaga tacagttttc tattcagata tgtttatttt aacattgttt
3361 ggttaaaaaa ggtgaagttc cagtcaacca ctttttaccc ctgaaatttc aagataatgc
3421 tatattaact tttccagatc taacactagc ttattcttcc ctgttataaa atggtttgaa
3481 cttactgagg agatattcct atcattaaca aaaataaact atttaaataa ccaaaaaaaa
3541 aaaaaaaa
//