LOCUS BC030545 2115 bp mRNA linear HUM 25-MAR-2004
DEFINITION Homo sapiens pleckstrin homology domain containing, family M (with
RUN domain) member 2, mRNA (cDNA clone IMAGE:5107529), partial cds.
ACCESSION BC030545
VERSION BC030545.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2115)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2115)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (07-MAY-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 64 Row: c Column: 24
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis.
FEATURES Location/Qualifiers
source 1..2115
/db_xref="H-InvDB:HIT000092142"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5107529"
/tissue_type="Cervix, carcinoma"
/clone_lib="NIH_MGC_12"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene <1..2115
/gene="PLEKHM2"
/gene_synonym="KIAA0842"
/db_xref="GeneID:23207"
CDS <1..1267
/gene="PLEKHM2"
/gene_synonym="KIAA0842"
/codon_start=2
/product="PLEKHM2 protein"
/protein_id="AAH30545.1"
/db_xref="GeneID:23207"
/translation="HASGENEEQLFKMIRMSTGHMEGNLQLLYVLLTDCYVYLLRKGA
TEKPYLVEEAVSYNELDYVSVGLDQQTVKLVCTNRRKQFLLDTADVALAEFFLASLKS
AMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEASAVTVRFYGLVHWEDPTDESL
GPTPCHCSPPEGTITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQYPDRTDVIPL
LSVNMGGEQCGGCRRANTTDRPHAFQVILSDRPCLELSAESEAEMAEWMQHLCQAVSK
GVIPQGVAPSPCIPCCLVLTDDRLFTCHEDCQTSFFRSLGTAKLGDISAVSTEPGKEY
CVLEFSQDSQQLLPPWVIYLSCTSELDRLLSALNSGWKTIYQVDLPHTAIQEASNKKK
FEDALSLIHSAWQRSDSLCRGRASRDPWC"
misc_feature 521..826
/gene="PLEKHM2"
/gene_synonym="KIAA0842"
/note="PH; Region: PH domain. PH stands for pleckstrin
homology"
/db_xref="CDD:pfam00169"
BASE COUNT 423 a 664 c 628 g 400 t
ORIGIN
1 ccacgcgtcc ggagaaaacg aagagcagct gttcaaaatg atccggatga gcaccgggca
61 catggagggc aacctgcagc tgctgtacgt gctgctcaca gactgctatg tctacctgct
121 ccggaaaggg gccacagaga agccatacct ggtggaagag gccgtttctt acaatgaact
181 tgactatgtg tcggttggcc ttgaccagca gacggtgaag ctggtgtgca ccaaccgcag
241 gaagcagttt ctgctggaca cggctgatgt ggcgctggct gagttctttt tggcttcttt
301 gaagtcagcc atgatcaaag gctgtcgaga acctccctac cccagcatcc tgacggatgc
361 caccatggag aagctggcac tggccaaatt tgtggcccaa gaatcgaagt gtgaggcatc
421 tgctgtcacc gtgcgcttct acggccttgt gcactgggag gaccccacag acgagtccct
481 gggccccacg ccctgccact gctcaccccc cgagggcacc atcaccaaag aaggcatgct
541 gcactacaag gcgggcacct cctacctggg caaggaacac tggaagacgt gcttcgtggt
601 gctcagcaac gggatcctct accagtaccc ggaccgcacc gacgtcatcc ctctgctctc
661 ggtgaacatg gggggggagc agtgcggtgg ctgccggaga gccaacacca cggatcggcc
721 ccacgccttc caggtcattc tctccgaccg gccctgcctg gagctaagtg ccgagagcga
781 ggccgagatg gccgagtgga tgcagcatct ctgccaggct gtgtccaaag gggtcatccc
841 ccagggcgta gctcccagcc cctgcatacc ctgctgcctg gtcctcacgg atgaccgcct
901 ctttacgtgc catgaggatt gccagaccag cttcttccgc tctttgggca cagccaagct
961 gggcgacatc agcgccgtct ccaccgagcc gggcaaggag tactgcgtct tggagttctc
1021 ccaggacagc cagcagctcc tcccgccctg ggtcatctac ctgagctgca cttctgaact
1081 ggaccgattg ctgtctgcac tgaactctgg gtggaaaacc atctatcagg tggacctccc
1141 ccacacggcg atccaggaag cctccaacaa gaagaaattc gaggatgcct tgagcctcat
1201 ccacagcgcc tggcagcgga gcgacagtct ctgccgcggc cgagcctccc gagacccctg
1261 gtgctgaggc agagctggtt ggcgtccctg gtgggcagga aaggaaggca cgccagccgg
1321 caggcacact gtcacggctg ttgtcatgct gtcgggagcc tacagtccac ccctgccctg
1381 ggcggcagaa ccaccgagtg tggcttaaga cagggtccct ccactccagg gatccagatc
1441 aggtgcccgg cacccctggg catcctgccc gacaggtagc gaatggaggt cgctgggggc
1501 agagggtccg agccctgtgg gctctgcgga tgcacgccct cctcccgggc ctccgcctca
1561 gtctgcagaa tttctgccga gtggcaccga gaacaccatc catctaagga cgaacaaaag
1621 aaccaggagg gcgggacccc cctcttcctc tcctgggttg ggggctgggg ccctgagtgc
1681 ccagccatcc ttgttcgtgt ttgaacactc tcctggccac gtggggaagc gggaacacgg
1741 ggtgtctgcg catgtttcct cctcctagct ccatcactgc gcacacagct gcctgcctcg
1801 ccagatgcag gggggcgggc agccctccct ggctgccagg aggctctgca tgcccacagt
1861 cctgccctgc ctgtcccctc aacccggcag tgcctgtagc accgaggagc aaagggggtg
1921 gatggggggc ttggagaagg gcggagccca ccagcctggc atccatgttg acatcttctg
1981 actgtcccct gcttggctgg agccaggccc ttccctagag tttcgtcaag agcctcctgg
2041 ggaaggggtc aggtggtttg ggttttgttt tttaaaataa aatagacatg ttatattgcc
2101 aaaaaaaaaa aaaaa
//