LOCUS BC028363 3708 bp mRNA linear HUM 21-JUL-2006
DEFINITION Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class
N, mRNA (cDNA clone MGC:26427 IMAGE:4826763), complete cds.
ACCESSION BC028363
VERSION BC028363.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3708)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3708)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (23-APR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC028363.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 33 Row: o Column: 23
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 34328904.
FEATURES Location/Qualifiers
source 1..3708
/db_xref="H-InvDB:HIT000040536"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:26427 IMAGE:4826763"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..3708
/gene="PIGN"
/gene_synonym="MCD4"
/gene_synonym="MDC4"
/gene_synonym="MGC26427"
/gene_synonym="PIG-N"
/db_xref="GeneID:23556"
/db_xref="HGNC:HGNC:8967"
/db_xref="MIM:606097"
CDS 386..3181
/gene="PIGN"
/gene_synonym="MCD4"
/gene_synonym="MDC4"
/gene_synonym="MGC26427"
/gene_synonym="PIG-N"
/codon_start=1
/product="phosphatidylinositol glycan anchor biosynthesis,
class N"
/protein_id="AAH28363.1"
/db_xref="GeneID:23556"
/db_xref="HGNC:HGNC:8967"
/db_xref="MIM:606097"
/translation="MLLFFTLGLLIHFVFFASIFDIYFTSPLVHGMTPQFTPLPPPAR
RLVLFVADGLRADALYELDENGNSRAPFIRNIIMHEGSWGISHTRVPTESRPGHVALI
AGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPDILPMFAKGASGDHVYTYSYD
AKREDFGAQDATKLDTWVFDNVKDFFHHARNNQSLFSKINEEKIVFFLHLLGIDTNGH
AHRPSSRDYKDNIKKVDDGVKEIVSMFNHFYGNDGKTTFIFTSDHGMTDWGSHGAGHP
SETLTPLVTWGAGIKYPQRVSAQQFDDAFLKEWRLENWKRLDVNQADIAPLMTSLIGV
PFPLNSVGILPVDYLNNTDLFKAESMFTNAVQILEQFKVKMTQKKEVTLPFLFTPFKL
LSDSKQFNILRKARSYIKHRKFDEVVSLCKELIHLALKGLSYYHTYDRFFLGVNVVIG
FVGWISYASLLIIKSHSNLIKGVSKEVKKPSHLLPCSFVAIGILVAFFLLIQACPWTY
YVYGLLPLPIWYAVLREFQVIQDLVVSVLTYPLSHFVGYLLAFTLGIEVLVLSFFYRY
MLTAGLTAFAAWPFLTRLWTRAKMTSLSWTFFSLLLAVFPLMPVVGRKPDISLVMGAG
LLVLLLSLCVVTSLMKRKDSFIKEELLVHLLQVLSTVLSMYVVYSTQSSLLRKQGLPL
MNQIISWATLASSLVVPLLSSPVLFQRLFSILLSLMSTYLLLSTGYEALFPLVLSCLM
FVWINIEQETLQQSGVCCKQKLTSIQFSYNTDITQFRQLYLDDIRRAFFLVFFLVTAF
FGTGNIASINSFDLASVYCFLTVFSPFMMGALMMWKILIPFVLVMCAFEAVQLTTQLS
SKSLFLIVLVISDIMALHFFFLVKDYGSWLDIGTSISHYVIVMSMTIFLVFLNGLAQL
LTTKKLRLCGKPKSHFM"
BASE COUNT 983 a 729 c 791 g 1205 t
ORIGIN
1 ggttgtagtg gtggcttagg aataccctcc cggctatcgc ttcagtgggt agaggggacc
61 actgcccgag agctttaatg gagctgggtc ctgccttcgc gctgaggagc cctcgttttc
121 gagatcaggc ctgaccggga taagctccag aaacagtatt atgtctcagt cctgtgcaaa
181 gtgagatgag ctgtagcctt tggagaccta taattttgaa catagatgtt ccttaaaagc
241 taaatctttt tgaacagatg atgataaacc tggaagagat tcaccaacaa tagcaaatta
301 cacactggct tggaaagtac tcccatgttc cgtttgatct tggcaattta atgctgttct
361 tgaagactaa ttagttacac tggatatgct gctgttcttt actttgggat tgcttataca
421 ttttgtgttc ttcgcctcca tctttgacat ttattttaca tctcctttgg ttcatggaat
481 gactcctcag tttacaccat tgcctcctcc agcgagaaga ttagtgttgt ttgttgctga
541 tggccttcga gcagatgcac tttacgaatt agatgaaaat ggaaactcta gagcaccgtt
601 tattaggaat atcataatgc atgaaggcag ctggggcata tctcatacac gtgtgccaac
661 agaatctcgg ccaggtcatg tagctctgat agctgggttt tatgaagatg tcagtgcagt
721 tgccaaagga tggaaggaaa atcctgtaga gtttgattct ctttttaatg aaagtaaata
781 cacatggagc tggggaagcc cagatatcct gcctatgttt gccaaaggtg ctagtggaga
841 ccacgtttat acatatagtt atgatgctaa aagagaggat tttggtgctc aagatgcaac
901 aaaactggat acgtgggttt ttgataatgt taaggacttc tttcatcatg ccagaaacaa
961 ccagtctttg ttttctaaaa taaatgaaga gaaaatagtt tttttcttac atttattagg
1021 aatagataca aacggacatg ctcatcgacc atcctcgaga gactacaagg acaatattaa
1081 aaaagttgat gatggagtta aagaaatcgt gtctatgttt aaccatttct atggaaatga
1141 tgggaaaaca acatttatct ttacctctga ccatggaatg acagactggg gttcccatgg
1201 ggctggtcat ccttcagaga ctttaactcc tttagtcact tggggagctg gaatcaagta
1261 tccccaaaga gtatcagctc agcaatttga tgatgcattt ttgaaagagt ggagattgga
1321 gaattggaag aggctagatg tcaatcaggc tgatattgca ccattgatga cttcccttat
1381 tggagttccc tttcctctta actcagtggg aatccttcct gtggattatc ttaacaacac
1441 tgatctcttc aaagcagaga gcatgtttac aaatgcagta cagattcttg aacagttcaa
1501 ggtgaaaatg actcagaaga aagaagttac tttaccattt ttgtttacac catttaaact
1561 gctttctgat tccaaacagt tcaacatttt aagaaaagca agatcttata taaaacacag
1621 aaagtttgat gaagtggtct ccctttgcaa ggagctaatt catcttgcat tgaaaggatt
1681 gtcctattat cacacatatg acagattctt tttgggcgtc aatgttgtta ttggttttgt
1741 gggatggata tcttatgcct ctttgttgat catcaagtct cattccaacc ttataaaagg
1801 tgttagtaaa gaagtgaaga aaccaagcca tctcctgcct tgtagttttg tagctattgg
1861 cattttagta gcattttttc tgctgattca agcctgtccc tggacatatt atgtatatgg
1921 tttgttgcca ctgccaatat ggtatgcggt tctaagagaa tttcaagtta ttcaggacct
1981 tgttgtatca gtgttgacct atcctctgag ccattttgtt gggtacctgt tagcctttac
2041 cctgggaatt gaagtattag ttctcagttt tttctaccgc tatatgctta ccgctggact
2101 tactgccttt gcagcttggc catttctcac tcggctgtgg actcgagcaa agatgacctc
2161 actgagttgg actttcttct ctttgctcct ggcagtgttc ccactgatgc cggttgtagg
2221 tcgaaagcca gacatctctc tagtgatggg tgcaggcttg ctggttcttc tgttatccct
2281 gtgtgttgta acatctctca tgaaaagaaa agatagcttt ataaaggaag agctattggt
2341 acatctgtta caggtgctga gcacagtgct ctccatgtat gttgtgtata gcactcagag
2401 tagtctactc aggaagcaag gactgcctct catgaatcaa attattagct gggcaacatt
2461 agcctcttcc ttggttgtgc cactactgag ttctccagtt ctctttcagc gattgttcag
2521 catacttctt tcattgatgt caacctacct acttctaagc acagggtatg aagctctctt
2581 tccactagtg ttgtcttgtt tgatgtttgt ctggataaac atagaacaag aaactctaca
2641 acaatctggt gtttgctgta aacaaaagct caccagtatc cagttctctt ataatactga
2701 tataactcag tttcgacagc tatatctgga tgacatccgt agggcctttt tccttgtttt
2761 cttcttagtg acagcatttt ttggaactgg aaatatagct tctattaaca gctttgatct
2821 tgcctctgtc tattgctttc tgactgtgtt cagtcctttt atgatgggag ccctgatgat
2881 gtggaagatt ttaatcccct ttgttcttgt tatgtgtgct tttgaagcag ttcagttgac
2941 tactcagtta tcgtcaaaaa gcctttttct cattgttctc gtcatatcag acattatggc
3001 tttgcatttt ttcttcttgg tcaaggatta tggcagctgg cttgatattg ggacaagcat
3061 cagccactat gtgattgtca tgtccatgac catctttttg gtgttcctca atggcctggc
3121 ccagctgctc acaacgaaga aactcagact atgtggcaaa cccaaaagtc acttcatgtg
3181 aggttgctga agcaccattc agcatctgga tcctgattct ccttttaagc taaaatctca
3241 tcaaggcttc aataagaaga tggatatgga tatatagtat attctactcc tgtaaggaaa
3301 atggtatttg gaattccgaa ttgacaggtt atctggaaca aaggagcttc cttttttttt
3361 ctaggttttg caggcatgaa atagtgatta tatctgtgga aaagcatagg aaggcattct
3421 cctttttcat ttttttcctt tggctggcag ctcttcccag tgatgttgag agcacctgca
3481 gcaatctggt ccccagtcgc acaacttccc acatacccag aggagagcat atgcctgtgg
3541 gggcagtgct gatggcatcc agagtcattg ctgtggctga gctggaagga aatcaccagg
3601 tgccaccgtc aatatttatc agctttcagc actggttttg ttagacagtc agggtgtatt
3661 atttcaagag cttcaataaa aaaagtgttt gcaaaaaaaa aaaaaaaa
//