LOCUS BC016285 1951 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens protein kinase, cAMP-dependent, catalytic, beta, mRNA
(cDNA clone MGC:9320 IMAGE:3916100), complete cds.
ACCESSION BC016285
VERSION BC016285.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1951)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1951)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-OCT-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 15 Row: k Column: 14
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 46909588.
FEATURES Location/Qualifiers
source 1..1951
/db_xref="H-InvDB:HIT000037513"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:9320 IMAGE:3916100"
/tissue_type="Skin, melanotic melanoma."
/clone_lib="NIH_MGC_72"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1951
/gene="PRKACB"
/gene_synonym="MGC41879"
/gene_synonym="MGC9320"
/gene_synonym="PKACB"
/db_xref="GeneID:5567"
/db_xref="HGNC:HGNC:9381"
/db_xref="MIM:176892"
CDS 183..956
/gene="PRKACB"
/gene_synonym="MGC41879"
/gene_synonym="MGC9320"
/gene_synonym="PKACB"
/codon_start=1
/product="protein kinase, cAMP-dependent, catalytic, beta"
/protein_id="AAH16285.1"
/db_xref="GeneID:5567"
/db_xref="HGNC:HGNC:9381"
/db_xref="MIM:176892"
/translation="MGNAATAKKGSEVESVKEFLAKAKEDFLKKWENPTQNNAGLEDF
ERKKTLGTGSFGRVMLVKHKATEQYYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFP
FLVRLEYAFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHS
LDLIYRDLKPENLLIDHQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNK
AVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKNF"
BASE COUNT 671 a 350 c 349 g 581 t
ORIGIN
1 gttcgctgga gccctttcct cagacccggc ccggtcttcg cgcccggact cctggcgcca
61 gcgctaggcg cactcaccgc tctgacgggt gcagacgcgg gagttgtccc agactgtgga
121 gtggcgggca cggccccagc cccccttccc ttccctgacc ccttcttgcc atcgccccag
181 acatggggaa cgcggcgacc gccaagaaag gcagcgaggt ggagagcgtg aaagagtttc
241 tagccaaagc caaagaagac tttttgaaaa aatgggagaa tccaactcag aataatgccg
301 gacttgaaga ttttgaaagg aaaaaaaccc ttggaacagg ttcatttgga agagtcatgt
361 tggtaaaaca caaagccact gaacagtatt atgccatgaa gatcttagat aagcagaagg
421 ttgttaaact gaagcaaata gagcatactt tgaatgagaa aagaatatta caggcagtga
481 attttccttt ccttgttcga ctggagtatg cttttaagga taattctaat ttatacatgg
541 ttatggaata tgtccctggg ggtgaaatgt tttcacatct aagaagaatt ggaaggttca
601 gtgagcccca tgcacggttc tatgcagctc agatagtgct aacattcgag tacctccatt
661 cactagacct catctacaga gatctaaaac ctgaaaatct cttaattgac catcaaggct
721 atatccaggt cacagacttt gggtttgcca aaagagttaa aggcagaact tggacattat
781 gtggaactcc agagtatttg gctccagaaa taattctcag caagggctac aataaggcag
841 tggattggtg ggcattagga gtgctaatct atgaaatggc agctggctat cccccattct
901 ttgcagacca accaattcag atttatgaaa agattgtttc tggaaagaac ttttgatatg
961 aacaaaacaa aactttgaga aaaattaaca gacaaggcag tgatttattt ttgaagaatt
1021 tgagaagtgt agactctcaa gaggactaaa ggtcatatga agaatgatga gagaaccaaa
1081 atacattaaa atcacaaatg gaagaagaat attttactaa tacaaaaact aagaatgtaa
1141 atgttataat aattgtttca aatcatttaa ttgacagtaa ttataaagtt cttgaatctt
1201 tactatatta cttttattta tacttcatat aagaaatcca gttttctaac aaggatactg
1261 tcataactaa atttacattt attaagaaaa actgctttag ttaaaattaa tgtgtcttca
1321 tttttatgca ttggcctcga tttgccaatc attctctatt ggttaaaatt tatattcagc
1381 tgtttatgaa tatatattca ttttatatca aactttaaaa ttttgtatct aataatcagc
1441 atatattcta aaatcataac agtctaaatc ctgggcacct tagaagaatg acaccagaaa
1501 accttattat atcacaatat tctgttttcc ccttcattta tttagaaata tgacaggata
1561 tttggtgtac ttttgttttt taactaaaag taccagattc tctctcccca tgtgggatat
1621 aaaattatcc ccatctctta ctccctttac tcatctaaag tagaagtcat gaaagtggaa
1681 tttttgccat taaaaggctc tgtattatgt gaagttagat tgtattaacc atttcccaat
1741 aaatcatctg tttcaaaact caaattcaaa ctagaatgtg tctctattca cattgcaaaa
1801 atattattgt ctctctggtt agtggctaaa agccaaattg gaaactaact agttttttaa
1861 attttttaaa ttgtgcaaat tattaaaaat ccaatttggt cttataaaaa aaaaaaaaaa
1921 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
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