LOCUS BC014439 2133 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens sphingosine kinase 1, mRNA (cDNA clone MGC:22956
IMAGE:4871343), complete cds.
ACCESSION BC014439
VERSION BC014439.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2133)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2133)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (17-SEP-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 34 Row: l Column: 9
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 41282001.
FEATURES Location/Qualifiers
source 1..2133
/db_xref="H-InvDB:HIT000050596"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:22956 IMAGE:4871343"
/tissue_type="Skin, melanotic melanoma, high MDR."
/clone_lib="NIH_MGC_49"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..2133
/gene="SPHK1"
/gene_synonym="SPHK"
/db_xref="GeneID:8877"
/db_xref="HGNC:HGNC:11240"
/db_xref="MIM:603730"
CDS 435..1847
/gene="SPHK1"
/gene_synonym="SPHK"
/codon_start=1
/product="sphingosine kinase 1"
/protein_id="AAH14439.1"
/db_xref="GeneID:8877"
/db_xref="HGNC:HGNC:11240"
/db_xref="MIM:603730"
/translation="MSAQVLGFLRSWTPLPLAAPRGPAAAGNDAGAPTATAPGGEGEP
HSRPCDARLGSTDKELKAGAAATGSAPTAPGTPWQREPRVEVMDPAGGPRGVLPRPCR
VLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDA
LVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNE
DLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEM
RFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSH
WTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAM
EKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSG
CVEPPPSWKPQQMPPPEEPL"
BASE COUNT 350 a 681 c 719 g 383 t
ORIGIN
1 gcgaggccgg ggagtccgct ccagcggggc gctccagtcc ctcagacgtg ggctgagctt
61 gggacgagct gcgttccgcc ccaggccact gtagggaacg gcggtggcgc ctccccagca
121 aaccggaccg actgggtagg gccgcccacc ctgccttcgc gccgctcgtg gctcctgtgc
181 ggcccgccct cgcggggccc cgggaactgg gccacttgtc gcttgggcga gagcaggcgg
241 cagctggtgg cccggttcgg gcttggcttt ggcgcgaccc gggagcggct cccacgagcg
301 ccgcgcgcgt cgcaacggag cggggccctg agaagcgcgc gccgcggctc ccaccgctct
361 ggagctccgg gcaggggaca cggcaacctg gatggctggg gcagggatcc tctcccagaa
421 cttcgtgccc agcaatgtcc gctcaagttc tgggattttt acgcagctgg actcccctcc
481 ccctggcagc cccgaggggt ccagccgccg cagggaatga cgccggtgct cctacagcca
541 cggctccggg cggggaaggc gagccccaca gccggccctg cgacgcccgc ctgggcagca
601 ccgataagga gctgaaggca ggagccgccg ccacgggcag cgcccccaca gcgccaggga
661 ccccctggca gcgggagccg cgggtcgagg ttatggatcc agcgggcggc ccccggggcg
721 tgctcccgcg gccctgccgc gtgctggtgc tgctgaaccc gcgcggcggc aagggcaagg
781 ccttgcagct cttccggagt cacgtgcagc cccttttggc tgaggctgaa atctccttca
841 cgctgatgct cactgagcgg cggaaccacg cgcgggagct ggtgcggtcg gaggagctgg
901 gccgctggga cgctctggtg gtcatgtctg gagacgggct gatgcacgag gtggtgaacg
961 ggctcatgga gcggcctgac tgggagaccg ccatccagaa gcccctgtgt agcctcccag
1021 caggctctgg caacgcgctg gcagcttcct tgaaccatta tgctggctat gagcaggtca
1081 ccaatgaaga cctcctgacc aactgcacgc tattgctgtg ccgccggctg ctgtcaccca
1141 tgaacctgct gtctctgcac acggcttcgg ggctgcgcct cttctctgtg ctcagcctgg
1201 cctggggctt cattgctgat gtggacctag agagtgagaa gtatcggcgt ctgggggaga
1261 tgcgcttcac tctgggcacc ttcctgcgtc tggcagccct gcgcacctac cgcggccgac
1321 tggcctacct ccctgtagga agagtgggtt ccaagacacc tgcctccccc gttgtggtcc
1381 agcagggccc ggtagatgca caccttgtgc cactggagga gccagtgccc tctcactgga
1441 cagtggtgcc cgacgaggac tttgtgctag tcctggcact gctgcactcg cacctgggca
1501 gtgagatgtt tgctgcaccc atgggccgct gtgcagctgg cgtcatgcat ctgttctacg
1561 tgcgggcggg agtgtctcgt gccatgctgc tgcgcctctt cctggccatg gagaagggca
1621 ggcatatgga gtatgaatgc ccctacttgg tatacgtgcc cgtggtcgcc ttccgtttgg
1681 agcccaagga tgggaaaggt gtgtttgcag tggatgggga attgatggtt agcgaggccg
1741 tgcagggcca ggtgcaccca aactacttct ggatggtcag cggttgcgtg gagcccccgc
1801 ccagctggaa gccccagcag atgccaccgc cagaagagcc cttatgaccc ctgggccgcg
1861 ctgtgcctta gtgtctactt gcaggaccct tcctccttcc ctagggctgc agggcctgtc
1921 cacagctcct gtgggggtgg aggagactcc tctggagaag ggtgagaagg tggaggctat
1981 gctttggggg gacaggccag aatgaagtcc tgggtcagga gcccagctgg ctgggcccag
2041 ctgcctatgt aaggccttct agtttgttct gagaccccca ccccacgaac caaatccaaa
2101 taaagtgaca ttcccaaaaa aaaaaaaaaa aaa
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