LOCUS BC012050 1936 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens actin, gamma 1, mRNA (cDNA clone MGC:19847
IMAGE:4554944), complete cds.
ACCESSION BC012050
VERSION BC012050.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1936)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1936)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (02-AUG-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 29 Row: d Column: 20
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 11038618.
FEATURES Location/Qualifiers
source 1..1936
/db_xref="H-InvDB:HIT000035619"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:19847 IMAGE:4554944"
/tissue_type="Uterus, leiomyosarcoma"
/clone_lib="NIH_MGC_46"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1936
/gene="ACTG1"
/gene_synonym="ACT"
/gene_synonym="beta-actin"
/db_xref="GeneID:71"
/db_xref="HGNC:HGNC:144"
/db_xref="MIM:102560"
CDS 60..1187
/gene="ACTG1"
/gene_synonym="ACT"
/gene_synonym="beta-actin"
/codon_start=1
/product="actin, gamma 1"
/protein_id="AAH12050.1"
/db_xref="GeneID:71"
/db_xref="HGNC:HGNC:144"
/db_xref="MIM:102560"
/translation="MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM
VGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP
VLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVT
HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCY
VALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHE
TTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPE
RKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF"
BASE COUNT 446 a 529 c 509 g 452 t
ORIGIN
1 gccaactctc gcactctgtt cttccgccgc tccgccgtcg cgtttctctg ccggtcgcaa
61 tggaagaaga gatcgccgcg ctggtcattg acaatggctc cggcatgtgc aaagctggtt
121 ttgctgggga cgacgctccc cgagccgtgt ttccttccat cgtcgggcgc cccagacacc
181 agggcgtcat ggtgggcatg ggccagaagg actcctacgt gggcgacgag gcccagagca
241 agcgtggcat cctgaccctg aagtacccca ttgagcatgg catcgtcacc aactgggacg
301 acatggagaa gatctggcac cacaccttct acaacgagct gcgcgtggcc ccggaggagc
361 acccagtgct gctgaccgag gcccccctga accccaaggc caacagagag aagatgactc
421 agattatgtt tgagaccttc aacaccccgg ccatgtacgt ggccatccag gccgtgctgt
481 ccctctacgc ctctgggcgc accactggca ttgtcatgga ctctggagac ggggtcaccc
541 acacggtgcc catctacgag ggctacgccc tcccccacgc catcctgcgt ctggacctgg
601 ctggccggga cctgaccgac tacctcatga agatcctcac tgagcgaggc tacagcttca
661 ccaccacggc cgagcgggaa atcgtgcgcg acatcaagga gaagctgtgc tacgtcgccc
721 tggacttcga gcaggagatg gccaccgccg catcctcctc ttctctggag aagagctacg
781 agctgcccga tggccaggtc atcaccattg gcaatgagcg gttccggtgt ccggaggcgc
841 tgttccagcc ttccttcctg ggtatggaat cttgcggcat ccacgagacc accttcaact
901 ccatcatgaa gtgtgacgtg gacatccgca aagacctgta cgccaacacg gtgctgtcgg
961 gcggcaccac catgtatccg ggcattgccg acaggatgca gaaggagatc accgccctgg
1021 cgcccagcac catgaagatc aagatcatcg cacccccaga gcgcaagtac tcggtgtgga
1081 tcggtggctc catcctggcc tcactgtcca ccttccagca gatgtggatt agcaagcagg
1141 agtacgacga gtcgggcccc tccatcgtcc accgcaaatg cttctaaacg gactcagcag
1201 atgcgtagca tttgctgcat gggttaattg agaatagaaa tttgcccctg gcaaatgcac
1261 acacctcatg ctagcctcac gaaactggaa taagccttcg aaaagaaatt gtccttgaag
1321 cttgtatctg atatcagcac tggattgtag aacttgttgc tgattttgac cttgtattga
1381 agttaactgt tccccttggt atttgtttaa taccctgtac atatctttga gttcaacctt
1441 tagtacgtgt ggcttggtca cttcgtggct aaggtaagaa cgtgcttgtg gaagacaagt
1501 ctgtggcttg gtgagtctgt gtggccagca gcctctgatc tgtgcagggt attaacgtgt
1561 cagggctgag tgttctggga tttctctaga ggctggcaag aaccagttgt tttgtcttgc
1621 gggtctgtca gggttggaaa gtccaagccg taggacccag tttcctttct tagctgatgt
1681 ctttggccag aacaccgtgg gctgttactt gctttgagtt ggaagcggtt tgcatttacg
1741 cctgtaaatg tattcattct taatttatgt aaggtttttt ttgtacgcaa ttctcgattc
1801 tttgaagaga tgacaacaaa ttttggtttt ctactgttat gtgagaacat taggccccag
1861 caacacgtca ttgtgtaagg aaaaataaaa gtgctgccgt aaccaaaaaa aaaaaaaaaa
1921 aaaaaaaaaa aaaaaa
//