LOCUS BC004914 1418 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens creatine kinase, brain, mRNA (cDNA clone MGC:4752
IMAGE:3537262), complete cds.
ACCESSION BC004914
VERSION BC004914.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1418)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1418)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (21-MAR-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 19, 2003 this sequence version replaced BC004914.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 11 Row: k Column: 12
This clone was selected for full length sequencing because it
passed the following selection criteria: Hexamer frequency ORF
analysis, Similarity but not identity to protein.
FEATURES Location/Qualifiers
source 1..1418
/db_xref="H-InvDB:HIT000032079"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:4752 IMAGE:3537262"
/tissue_type="Lung, small cell carcinoma"
/clone_lib="NIH_MGC_7"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1418
/gene="CKB"
/gene_synonym="B-CK"
/db_xref="GeneID:1152"
/db_xref="HGNC:HGNC:1991"
/db_xref="MIM:123280"
CDS 58..1203
/gene="CKB"
/gene_synonym="B-CK"
/codon_start=1
/product="creatine kinase, brain"
/protein_id="AAH04914.1"
/db_xref="GeneID:1152"
/db_xref="HGNC:HGNC:1991"
/db_xref="MIM:123280"
/translation="MPFSNSHNALKLRFPAEDEFPDLSAHNNHMAKVLTPELYAELRA
KSTPSGFTLDDVIQTGVDNPGHPYIMTVGCVAGDEESYEVFKDLFDPIIEDRHGGYKP
SDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRAIEKLAVE
ALSSLDGDLAGRYYALKSMTEAEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIW
HNDNKTFLVWVNEEDHLRVISMQKGGNMKEVFTRFCTGLTQIETLFKSKDYEFMWNPH
LGYILTCPSNLGTGLRAGVHIKLPNLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVF
DVSNADRLGFSEVELVQMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK"
BASE COUNT 295 a 468 c 423 g 232 t
ORIGIN
1 gagctgccga cggacggagc gcccccgccc ccgcccggcc gcccgcccgc cgccgccatg
61 cccttctcca acagccacaa cgcactgaag ctgcgcttcc cggccgagga cgagttcccc
121 gacctgagcg cccacaacaa ccacatggcc aaggtgctga cccccgagct gtacgcggag
181 ctgcgcgcca agagcacgcc gagcggcttc acgctggacg acgtcatcca gacaggcgtg
241 gacaacccgg gccacccgta catcatgacc gtgggctgcg tggcgggcga cgaggagtcc
301 tacgaagtgt tcaaggatct cttcgacccc atcatcgagg accggcacgg cggctacaag
361 cccagcgatg agcacaagac cgacctcaac cccgacaacc tgcagggcgg cgacgacctg
421 gaccccaact acgtgctgag ctcgcgggtg cgcacgggcc gcagcatccg tggcttctgc
481 ctccccccgc actgcagccg cggggagcgc cgagccatcg agaagctcgc ggtggaagcc
541 ctgtccagcc tggacggcga cctggcgggc cgatactacg cgctcaagag catgacggag
601 gcggagcagc agcagctcat cgacgaccac ttcctcttcg acaagcccgt gtcgcccctg
661 ctgctggcct cgggcatggc ccgcgactgg cccgacgccc gcggtatctg gcacaatgac
721 aataagacct tcctggtgtg ggtcaacgag gaggaccacc tgcgggtcat ctccatgcag
781 aaggggggca acatgaagga ggtgttcacc cgcttctgca ccggcctcac ccagattgaa
841 actctcttca agtctaagga ctatgagttc atgtggaacc ctcacctggg ctacatcctc
901 acctgcccat ccaacctggg caccgggctg cgggcaggtg tgcatatcaa gctgcccaac
961 ctgggcaagc atgagaagtt ctcggaggtg cttaagcggc tgcgacttca gaagcgaggc
1021 acaggcggtg tggacacggc tgcggtgggc ggggtcttcg acgtctccaa cgctgaccgc
1081 ctgggcttct cagaggtgga gctggtgcag atggtggtgg acggagtgaa gctgctcatc
1141 gagatggaac agcggctgga gcagggccag gccatcgacg acctcatgcc tgcccagaaa
1201 tgaagcccgg cccacacccg acaccagccc tgctgcttcc taacttattg cctgggcagt
1261 gcccaccatg cacccctgat gttcgccgtc tggcgagccc ttagccttgc tgtagagact
1321 tccgtcaccc ttggtagagt ttattttttt gatggctaag atactgctga tgctgaaata
1381 aactagggtt ttggcctgaa aaaaaaaaaa aaaaaaaa
//