LOCUS BC000292 1940 bp mRNA linear HUM 15-JUL-2006 DEFINITION Homo sapiens actin, gamma 1, mRNA (cDNA clone MGC:8332 IMAGE:2819345), complete cds. ACCESSION BC000292 VERSION BC000292.2 KEYWORDS MGC. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1940) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. CONSRTM Mammalian Gene Collection Program Team TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (bases 1 to 1940) CONSRTM NIH MGC Project TITLE Direct Submission JOURNAL Submitted (15-NOV-2000) National Institutes of Health, Mammalian Gene Collection (MGC), Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT On Aug 19, 2003 this sequence version replaced BC000292.1. Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: DCTD/DTP cDNA Library Preparation: Rubin Laboratory cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: National Institutes of Health Intramural Sequencing Center (NISC), Gaithersburg, Maryland; Web site: http://www.nisc.nih.gov/ Contact: nisc_mgc@nhgri.nih.gov Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B., Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S., Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P., Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R., Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C., McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W., Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L., Young,A., Zhang,L.-H. and Green,E.D. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAL Plate: 1 Row: a Column: 11 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 11038618. FEATURES Location/Qualifiers source 1..1940 /db_xref="H-InvDB:HIT000029492" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:8332 IMAGE:2819345" /tissue_type="Lung, small cell carcinoma" /clone_lib="NIH_MGC_7" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 1..1940 /gene="ACTG1" /gene_synonym="ACT" /gene_synonym="beta-actin" /db_xref="GeneID:71" /db_xref="HGNC:HGNC:144" /db_xref="MIM:102560" CDS 66..1193 /gene="ACTG1" /gene_synonym="ACT" /gene_synonym="beta-actin" /codon_start=1 /product="actin, gamma 1" /protein_id="AAH00292.1" /db_xref="GeneID:71" /db_xref="HGNC:HGNC:144" /db_xref="MIM:102560" /translation="MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM VGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP VLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVT HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCY VALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHE TTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPE RKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF" BASE COUNT 445 a 530 c 512 g 453 t ORIGIN 1 gccgctgcca gctctcgcac tctgtttttc cgccgctccg ccgtcgcgtt tctctgccgg 61 tcgcaatgga agaagagatc gccgcgctgg tcattgacaa tggctccggc atgtgcaaag 121 ctggttttgc tggggacgac gctccccgag ccgtgtttcc ttccatcgtc gggcgcccca 181 gacaccaggg cgtcatggtg ggcatgggcc agaaggactc ctacgtgggc gacgaggccc 241 agagcaagcg tggcatcctg accctgaagt accccattga gcatggcatc gtcaccaact 301 gggacgacat ggagaagatc tggcaccaca ccttctacaa cgagctgcgc gtggccccgg 361 aggagcaccc agtgctgctg accgaggccc ccctgaaccc caaggccaac agagagaaga 421 tgactcagat tatgtttgag accttcaaca ccccggccat gtacgtggcc atccaggccg 481 tgctgtccct ctacgcctct gggcgcacca ctggcattgt catggactct ggagacgggg 541 tcacccacac ggtgcccatc tacgagggct acgccctccc ccacgccatc ctgcgtctgg 601 acctggctgg ccgggacctg accgactacc tcatgaagat cctcactgag cgaggctaca 661 gcttcaccac cacggccgag cgggaaatcg tgcgcgacat caaggagaag ctgtgctacg 721 tcgccctgga cttcgagcag gagatggcca ccgccgcatc ctcctcttct ctggagaaga 781 gctacgagct gcccgatggc caggtcatca ccattggcaa tgagcggttc cggtgtccgg 841 aggcgctgtt ccagccttcc ttcctgggta tggaatcttg cggcatccac gagaccacct 901 tcaactccat catgaagtgt gacgtggaca tccgcaaaga cctgtacgcc aacacggtgc 961 tgtcgggcgg caccaccatg tacccgggca ttgccgacag gatgcagaag gagatcaccg 1021 ccctggcgcc cagcaccatg aagatcaaga tcatcgcacc cccagagcgc aagtactcgg 1081 tgtggatcgg tggctccatc ctggcctcac tgtccacctt ccagcagatg tggattagca 1141 agcaggagta cgacgagtcg ggcccctcca tcgtccaccg caaatgcttc taaacggact 1201 cagcagatgc gtagcatttg ctgcatgggt taattgagaa tagaaatttg cccctggcaa 1261 atgcacacac ctcatgctag cctcacgaaa ctggaataag ccttcgaaaa gaaattgtcc 1321 ttgaagcttg tatctgatat cagcactgga ttgtagaact tgttgctgat tttgaccttg 1381 tattgaagtt aactgttccc cttggtattt gtttaatacc ctgtacatat ctttgagttc 1441 aacctttagt acgtgtggct tggtcacttc gtggctaagg taagaacgtg cttgtggaag 1501 acaagtctgt ggcttggtga gtctgtgtgg ccagcagcct ctgatctgtg cagggtatta 1561 acgtgtcagg gctgagtgtt ctgggatttc tctagaggct ggcaagaacc agttgttttg 1621 tcttgcgggt ctgtcagggt tggaaagtcc aagccgtagg acccagtttc ctttcttagc 1681 tgatgtcttt ggccagaaca ccgtgggctg ttacttgctt tgagttggaa gcggtttgca 1741 tttacgcctg taaatgtatt cattcttaat ttatgtaagg ttttttttgt acgcaattct 1801 cgattctttg aagagatgac aacaaatttt ggttttctac tgttatgtga gaacattagg 1861 ccccagcaac acgtcattgt gtaaggaaaa ataaaagtgc tgccgtaaaa aaaaaaaaaa 1921 aaaaaaaaaa aaaaaaaaaa //