LOCUS AK312700 1372 bp mRNA linear HTC 24-MAY-2008
DEFINITION Homo sapiens cDNA, FLJ93099, Homo sapiens pyruvate dehydrogenase
kinase, isoenzyme 1 (PDK1), nuclear gene encoding mitochondrial
protein, mRNA.
ACCESSION AK312700
VERSION AK312700.1
KEYWORDS HTC; HTC_FLI; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1372)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Kaida,T., Tsuchiya,K.,
Iida,Y., Takayama,Y., Murakawa,K., Kanehori,K., Andoh,T.,
Kagawa,N., Sato,R., Kawamura,Y., Tanaka,S., Kisu,Y., Sugano,S.,
Goshima,N., Nomura,N. and Isogai,T.
TITLE NEDO functional analysis of protein and research application
project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC and Biological
Information Research Center (BIRC), AIST; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1372
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3009530"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000431566"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 62..1372
/note="Homo sapiens pyruvate dehydrogenase kinase,
isoenzyme 1 (PDK1), nuclear gene encoding mitochondrial
protein, mRNA"
/protein_id="BAG35578.1"
/translation="MRLARLLRGAALAGPGPGLRAAGFSRSFSSDSGSSPASERGVPG
QVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLL
RTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTMAQGV
IEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHIGS
INPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHM
VFELFKNAMRATMEHHANRGVYPPIQVHVTLGNEDLTVKMSDRGGGVPLRKIDRLFNY
MYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKAL
STDSIERLPVYNKAAWKHYNTNHEADDWCVPSREPKDMTTFRSA"
BASE COUNT 360 a 332 c 338 g 342 t
ORIGIN
1 actcggcaga ggcgcgggga aacctggcgt actggctgtg gcttctctag cgggactcgg
61 catgaggctg gcgcggctgc ttcgcggagc cgccttggcc ggcccgggcc cggggctgcg
121 cgccgccggc ttcagccgca gcttcagctc ggactcgggc tccagcccgg cgtccgagcg
181 cggcgttccg ggccaggtgg acttctacgc gcgcttctcg ccgtccccgc tctccatgaa
241 gcagttcctg gacttcggat cagtgaatgc ttgtgaaaag acctcattta tgtttctgcg
301 gcaagagttg cctgtcagac tggcaaatat aatgaaagaa ataagtctcc ttccagataa
361 tcttctcagg acaccatccg ttcaattggt acaaagctgg tatatccaga gtcttcagga
421 gcttcttgat tttaaggaca aaagtgctga ggatgctaaa gctatttatg actttacaga
481 tactgtgata cggatcagaa accgacacaa tgatgtcatt cccacaatgg cccagggtgt
541 gattgaatac aaggagagct ttggggtgga tcctgtcacc agccagaatg ttcagtactt
601 tttggatcga ttctacatga gtcgcatttc aattagaatg ttactcaatc agcactcttt
661 attgtttggt ggaaaaggca aaggaagtcc atctcatcga aaacacattg gaagcataaa
721 tccaaactgc aatgtacttg aagttattaa agatggctat gaaaatgcta ggcgtctgtg
781 tgatttgtat tatattaact ctcccgaact agaacttgaa gaactaaatg caaaatcacc
841 aggacagcca atacaagtgg tttatgtacc atcccatctc tatcacatgg tgtttgaact
901 tttcaagaat gcaatgagag ccactatgga acaccatgcc aacagaggtg tttacccccc
961 tattcaagtt catgtcacgc tgggtaatga ggatttgact gtgaagatga gtgaccgagg
1021 aggtggcgtt cctttgagga aaattgacag acttttcaac tacatgtatt caactgcacc
1081 aagacctcgt gttgagacct cccgcgcagt gcctctggct ggttttggtt atggattgcc
1141 catatcacgt ctttacgcac aatacttcca aggagacctg aagctgtatt ccctagaggg
1201 ttacgggaca gatgcagtta tctacattaa ggctctgtca acagactcaa tagaaagact
1261 cccagtgtat aacaaagctg cctggaagca ttacaacacc aaccacgagg ctgatgactg
1321 gtgcgtcccc agcagagaac ccaaagacat gacgacgttc cgcagtgcct ag
//