LOCUS AK218444 247 bp mRNA linear HTC 19-SEP-2008 DEFINITION Mus musculus cDNA, clone:Y2G0145D16, strand:plus, reference:ENSEMBL:Mouse-Transcript-ENST:ENSMUST00000027802, based on BLAT search. ACCESSION AK218444 VERSION AK218444.1 KEYWORDS HTC; ASSETS. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 247) AUTHORS Arakawa,T., Carninci,P., Fukuda,S., Harbers,M., Hayatsu,N., Hori,F., Imotani,K., Kawai,J., Kondo,S., Murata,M., Nakamura,M., Nomura,K., Ohno,M., Sasaki,D., Shiraki,T., Waki,K., Watahiki,A. and Hayashizaki,Y. TITLE Direct Submission JOURNAL Submitted (15-SEP-2004) to the DDBJ/EMBL/GenBank databases. Contact:Yoshihide Hayashizaki The Institute of Physical and Chemical Research (RIKEN), Omics Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan URL :http://www.osc.riken.jp/ REFERENCE 2 AUTHORS Watahiki,A., Waki,K., Hayatsu,N., Shiraki,T., Kondo,S., Nakamura,M., Sasaki,D., Arakawa,T., Kawai,J., Harbers,M., Hayashizaki,Y. and Carninci,P. TITLE Libraries enriched for alternatively spliced exons reveal splicing patterns in melanocytes and melanomas JOURNAL Nat. Methods 1, 233-239 (2004) COMMENT Alternative Splicing Libraries (ASLs) are prepared by: Preparating of single-stranded DNA using a RNA template from full length cDNA libraries, hybridizating of single-stranded DNAs, removing of remaining single-stranded DNA, digesting of regions comprising double-stranded DNA by a set of 4 bp-cutters, capturing of DNA hybrids with loop structures (alternative spliced exon), ligating of Y-shaped primers to isolated DNA hybrids with loop structures, PCR amplification of ligation products and their cloning into pFLCI vector. (Reference) FEATURES Location/Qualifiers source 1..247 /cell_line="mixture of B16-F10Y and melan-c" /cell_type="mixture of melanoma cell and melanocyte cell" /clone="Y2G0145D16" /clone_lib="Alternative Splicing Library L3" /db_xref="taxon:10090" /mol_type="mRNA" /note="strand:plus, reference:ENSEMBL:Mouse-Transcript- ENST:ENSMUST00000027802, based on BLAT search" /organism="Mus musculus" BASE COUNT 63 a 44 c 70 g 70 t ORIGIN 1 cggaagtctg tggagagctt tagttagatc cgaggctgct tcagattgat ctccaggtga 61 ggtgagagga aacgggaatc ctctgaatta aaaacacgca aagaagcctg tgtcttggtg 121 ttgcaggtgg aagtgtgggg agtcatcctg tgacctcttg cttaactctt ctggacaggt 181 gacccattag aaggtgatgt tcagggctgc tttaataaag atcttattta ttttcgaaaa 241 cagtccg //