LOCUS AK090598 2552 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ33279 fis, clone ASTRO2008895, highly similar
to Lactase-like protein precursor.
ACCESSION AK090598
VERSION AK090598.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2552)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (04-JUL-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Kawakami,B., Sugiyama,A., Takemoto,M., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Suzuki,Y., Sugano,S., Nagahari,K., Masuho,Y., Nagai,K.
and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: HRI and RAB; annotation: HRI and RAB.
FEATURES Location/Qualifiers
source 1..2552
/cell_type="normal astrocytes (NHA5732)"
/clone="ASTRO2008895"
/clone_lib="ASTRO2"
/db_xref="H-InvDB:HIT000015465"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal astrocytes"
/organism="Homo sapiens"
CDS 346..1530
/codon_start=1
/note="highly similar to Lactase-like protein precursor"
/protein_id="BAG52192.1"
/translation="MANYFRDYANLCFEAFGDRVKHWITFSDPRAMAEKGYETGHHAP
GLKLRGTGLYKAAHHIIKAHAKAWHSYNTTWRSKQQGLVGISLNCDWGEPVDISNPKD
LEAAERYLQFCLGWFANPIYAGDYPQVMKDYIGRKSAEQGLEMSRLPVFSLQEKSYIK
GTSDFLGLGHFTTRYITERNYPSRQGPSYQNDRDLIELVDPNWPDLGSKWLYSVPWGF
RRLLNFAQTQYGDPPIYVMENGASQKFHCTQLCDEWRIQYLKGYINEMLKAIKDGANI
KGYTSWSLLDKFEWEKGYSDRYGFYYVEFNDRNKPRYPKASVQYYKKIIIANGFPNPR
EVESWYLKALETCSINNQMLAAEPLLSHMQMVTEIVVPTVCSLCVLITAVLLMLLLRR
QS"
BASE COUNT 731 a 563 c 576 g 682 t
ORIGIN
1 gctgtggctc ctctctcagg ggacagcgta ggccctgggg gtcagttgga gagggctctg
61 actaccagcg actgctctgg gggtgtctgc gatcaaggac gatcctggga ggacatcatt
121 ctgctgaggg aactgcacgt caaccactac cgattctccc tgtcttggcc ccggctcctg
181 cccacaggca tccgagccga gcaggtgaac aagaagggaa tcgaattcta cagtgatctt
241 atcgatgccc ttctgagcag caacatcact cccatcgtga ccttgcacca ctgggatctg
301 ccacagctgc tccaggtcaa atacggtggg tggcagaatg tgagcatggc caactacttc
361 agagactacg ccaacctgtg ctttgaggcc tttggggacc gtgtgaagca ctggatcacg
421 ttcagtgatc ctcgggcaat ggcagaaaaa ggctatgaga cgggccacca tgcgccgggc
481 ctgaagctcc gcggcaccgg cctgtacaag gcagcacacc acatcattaa ggcccacgcc
541 aaagcctggc attcttataa caccacgtgg cgcagcaagc agcaaggtct ggtgggaatt
601 tcattgaact gtgactgggg ggaacctgtg gacattagta accccaagga cctagaggct
661 gccgagagat acctacagtt ctgtctgggc tggtttgcca accccattta tgccggtgac
721 tacccccaag tcatgaagga ctacattgga agaaagagtg cagagcaagg cctggagatg
781 tcgaggttac cggtgttctc actccaggag aagagctaca ttaaaggcac atccgatttc
841 ttgggattag gtcattttac tactcggtac atcacggaaa ggaactaccc ctcccgccag
901 gggcccagct accagaacga tcgtgacttg atagagctgg ttgacccaaa ctggccagat
961 ctggggtcta aatggctata ttctgtgcca tggggattta ggaggctcct taactttgct
1021 cagactcaat acggtgatcc tcccatatat gtgatggaaa atggagcatc tcaaaaattc
1081 cactgtactc aattatgtga tgagtggaga attcaatacc ttaaaggata cataaatgaa
1141 atgctaaaag ctataaaaga tggtgctaat ataaaggggt atacttcctg gtctctgttg
1201 gataagtttg aatgggagaa aggatactca gatagatatg gattctacta tgttgaattt
1261 aacgacagaa ataagcctcg ctatccaaag gcttcagttc aatattacaa gaagattatc
1321 attgccaatg ggtttcccaa tccaagagag gtggaaagtt ggtacctcaa agctttggaa
1381 acttgctcta tcaacaatca gatgcttgct gcagagccct tgctaagtca catgcaaatg
1441 gttacggaga tcgtggtacc cactgtctgc tccctctgtg tcctcatcac tgctgttcta
1501 ctaatgctcc tcctgaggag gcagagctga gacaggatta tcaattttgg agcttcataa
1561 gagaatcttc aggatcttcc tcccttttct gctttgaggg tttccataca ttgctgtttt
1621 caggttctac aataattacc tttttttctc tttctctttt tggcttgtgc tgggatttaa
1681 gaattagaaa ataaaaataa gcagaaatta aaaaaaaaaa cacttgtaat ctcacccctc
1741 ctaaagttaa tgttaagatt ttgctgtata taatgaatgt atattatgaa ttatttacaa
1801 aaatggcatc atagtataca aactgttgtg acttgattgt ttatcactct aaaatctgtc
1861 cagaaatatt actttaatgt tcaaatgatt gccatctcag cactgtccca tacctgatag
1921 ataaaatcaa tctggctggg cacagtggct cacacctgta atcccagcac tttgggaggc
1981 tgaggctggt gaatcacttg aggccagaag tttgagacca gcctggccaa catggttaaa
2041 ccctgtctct actaaaaata tagaaactag ctgggcatgg tggcgggcac ctgtaatccc
2101 atttacttgg gaggctgagg caggagaatc gcttgaacct gggaggtgaa ggttgcagtg
2161 agctgagatc acaccactgc actccagcct gtgcaacaga gaaagactcc gtttcaaaag
2221 agaaaagaaa agaaatctat agataacaat ccttccaggt tgtaaatatt attagccaac
2281 cacagaaaag catgaagtgg gtaccaagta aatgagcacg tacactcttc tgacacttta
2341 gtctgaattt agtctgacaa ctctgactaa attcaactta ctttagtcaa tgaaatacct
2401 caaagaaata ttacatgttt attttttatt tatcttctag gtaactcaat atcgtctttt
2461 ttataataaa gattcatttt actcttaact atgttaaaat gctaaaatta attaatttcc
2521 atttatttgg ggataaaaca tggcttaaga ac
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