LOCUS AK056086 2006 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ31524 fis, clone NT2RI2000284, highly similar
to AMSH-like protease (EC 3.1.2.15).
ACCESSION AK056086
VERSION AK056086.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2006)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2006
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2000284"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012700"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 435..1745
/codon_start=1
/note="highly similar to AMSH-like protease (EC 3.1.2.15)"
/protein_id="BAG51619.1"
/translation="MDQPFTVNSLKKLAAMPDHTDVSLSPEERVRALSKLGCNITISE
DITPRRYFRSGVEMERMASVYLEEGNLENAFVLYNKFITLFVEKLPNHRDYQQCAVPE
KQDIMKKLKEIAFPRTDELKNDLLKKYNVEYQEYLQSKNKYKAEILKKLEHQRLIEAE
RKRIAQMRQQQLESEQFLFFEDQLKKQELARGQMRSQQTSGLSEQIDGSALSCFSTHQ
NNSLLNVFADQPNKSDATNYASHSPPVNRALTPAATLSAVQNLVVEGLRCVVLPEDLC
HKFLQLAESNTVRGIETCGILCGKLTHNEFTITHVIVPKQSAGPDYCDMENVEELFNV
QDQHDLLTLGWIHTHPTQTAFLSSVDLHTHCSYQLMLPEAIAIVCSPKHKDTGIFRLT
NAGMLEVSACKKKGFHPHTKEPRLFSICKHVLVKDIKIIVLDLR"
BASE COUNT 587 a 439 c 484 g 496 t
ORIGIN
1 attttcctgc agcagcggca gcagggacgg ttgctgcagg ttcggggtcg gccggcctgc
61 gcgtgggctt gcgaggacgc tgttcgtccc ctgcgctggg gtgtccgaca gcgaggagga
121 gaacgacgca cggagcccgc gcgactggaa ccagcaaagc tccatctgtc ggcagaggag
181 aagggggagg aggcacggcc gaggcaaacg agcggacgcc tcgtcgccgg gtgccggtat
241 caccccgctg caacgccttc cagcaaaagc caccgcggcc cgggttgcag cagccggacg
301 gatgccaagg ccacacggca gccacggggg cagccgtcgc agtcgccgtc ccacacgggc
361 tgcggacacc aagggttgct aatgaagtga ttgagaagaa acagtgaaca tcctcatttc
421 acagataaga caacatggat cagcctttta ctgtgaattc tctgaaaaag ttagctgcta
481 tgcctgacca tacagatgtt tccctaagcc cagaagagcg agtccgtgcc ctaagcaagc
541 ttggttgtaa tatcaccatc agtgaagaca tcactccacg acgttacttt aggtctggag
601 tagagatgga gaggatggcg tctgtgtatt tggaagaagg aaatttggaa aatgcctttg
661 ttctttataa taaatttata accttatttg tagaaaagct tcctaaccat cgagattacc
721 agcaatgtgc agtacctgaa aagcaggata ttatgaagaa actgaaggag attgcattcc
781 caaggacaga tgaattgaaa aacgaccttt taaagaaata taacgtagaa taccaagaat
841 atttgcaaag caaaaacaaa tataaagctg aaattctcaa aaaattggag catcagagat
901 tgatagaggc agaaaggaag cggattgctc agatgcgcca gcagcagcta gaatcggagc
961 agtttctgtt tttcgaagat caactcaaga agcaagagtt agcccgaggt caaatgcgaa
1021 gtcagcaaac ctcagggctg tcagagcaga ttgatgggag cgctttgtcc tgcttttcca
1081 cacaccagaa caattccttg ctgaatgtat ttgcagatca acctaataaa agtgatgcaa
1141 ccaattatgc tagccactct cctcctgtaa acagggcctt aacgccagct gctactctaa
1201 gtgctgttca gaatttagtg gttgaaggac tgcgatgtgt agttttgcca gaagatcttt
1261 gccacaaatt tctgcaactg gcagaatcta atacagtgag aggaatagaa acctgtggaa
1321 tactctgtgg aaaactgaca cataatgaat ttactattac ccatgtaatt gtgccaaagc
1381 agtctgcggg accagactat tgtgacatgg agaatgtaga ggaattattc aatgttcagg
1441 atcaacatga tctcctcact ctaggatgga tccatacaca tcccactcaa actgcatttt
1501 tatccagcgt tgatcttcac actcactgtt cctatcaact catgttgcca gaggccattg
1561 ccattgtttg ctcaccaaag cataaagaca ctggcatctt caggctcacc aatgctggca
1621 tgcttgaggt ttctgcttgt aaaaaaaagg gctttcatcc acacaccaag gagcccaggc
1681 tgttcagtat atgcaaacat gtgttggtaa aagacataaa aataattgtg ttggatctga
1741 ggtgatatgt tctgaatgta agcaccgtca acatcagaca cctactcatg gacatgtggt
1801 tgccggattt tcttaagatg tttccagaaa tgactgatat tttatattta tacattttag
1861 atgacaaagc ttgatattta ttgctgttgc acattttaaa gttttctttt tgggttgctc
1921 tgtgtcaaga gaggttacat ggtgttaaat cggtacctga taatgtaccc aaatactatg
1981 gccagataat aaattgtgct gcaaac
//