LOCUS AF214454 3404 bp mRNA linear HUM 11-JUL-2001
DEFINITION Homo sapiens alkaline phytoceramidase (APHC) mRNA, complete cds.
ACCESSION AF214454
VERSION AF214454.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3404)
AUTHORS Mao,C., Xu,R., Szulc,Z.M., Bielawska,A., Galadari,S.H. and
Obeid,L.M.
TITLE Cloning and characterization of a novel human alkaline ceramidase.
A mammalian enzyme that hydrolyzes phytoceramide
JOURNAL J. Biol. Chem. 276 (28), 26577-26588 (2001)
PUBMED 11356846
REFERENCE 2 (bases 1 to 3404)
AUTHORS Mao,C., Xu,R. and Obeid,L.M.
TITLE Direct Submission
JOURNAL Submitted (09-DEC-1999) Medicine, Medical University of SC, 114
Doughty Street, Charleston, SC 29425, USA
FEATURES Location/Qualifiers
source 1..3404
/db_xref="H-InvDB:HIT000074114"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="11"
/tissue_type="kidney"
gene 1..3404
/gene="APHC"
/note="hAPHC"
CDS 59..862
/gene="APHC"
/function="hydrolyzes phytoceramide into phytosphingosine
and fatty acid"
/note="aPHC; similar to Saccharomyces cerevisiae alkaline
ceramidase YPC1p but lacks reverse activity of
synthesizing phytoceramide from phytosphingosine and a
fatty acid"
/codon_start=1
/product="alkaline phytoceramidase"
/protein_id="AAK71923.1"
/translation="MAPAADREGYWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMI
IPPMFGAIQSVRDGLEKRYIASYLALTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCI
FVYCMFECFKIKNSVNYHLLFTLVLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLRS
IYIVTWVYPWLRGLGYTSLGIFLLGFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHA
WWHILTGLGSYLHILFSLYTRTLYLRYRPKVKFLFGIWPVILFEPLRKH"
BASE COUNT 980 a 690 c 667 g 1065 t
ORIGIN
1 tcgccagcct aacccggcac agtgagcgga gcgcctgggc ggcggcggcg gcggcgtgat
61 ggctccggcc gcggaccgag agggctactg gggccccacg acctccacgc tggactggtg
121 cgaggagaac tactccgtga cctggtacat cgccgagttc tggaatacag tgagtaacct
181 gatcatgatt atacctccaa tgttcggtgc aattcagagt gttagagacg gtctggaaaa
241 acggtacatt gcttcttatt tagcactcac agtggtagga atgggatcct ggtgcttcca
301 catgactctg aaatatgaaa tgcagctatt ggatgaactc ccaatgatat acagctgttg
361 catatttgtg tactgcatgt ttgaatgttt caagatcaag aactcagtaa actaccatct
421 gctttttacc ttagttctat tcagtttaat agtaaccaca gtttacctta aggtaaaaga
481 gccaatattc catcaggtca tgtatggaat gttggtcttt acattagtac ttcgatctat
541 ttatattgtt acatgggttt atccatggct tagaggactg ggttatacat cattgggtat
601 atttttattg ggatttttat tttggaatat agataacata ttttgtgagt cactgaggaa
661 ctttcgaaag aaggtaccac ctatcatagg tattaccaca caatttcatg catggtggca
721 tattttaact ggccttggtt cctatcttca catccttttc agtttgtata caagaacact
781 ttacctgaga tataggccaa aagtgaagtt tctctttgga atctggccag tgatcctgtt
841 tgagcctctc aggaagcatt gatgaatcat tccaccaaga aaacaaacaa gcacctacca
901 tagacctggc agaataaata aggaaatcct taaagatcta caagttcaaa tatgtcatga
961 ccatcacagc agaggagtga ctttctgact aatgctgcca cccacacaga gaataaggag
1021 tagggcctgc tgggtgttta gctcatggct ttatcttatt tgtccccctc ctcctttcac
1081 gctccagttt ataaagaaac agagatgatg tgtgtgtatg cctcaaatgc agaaacagtt
1141 gggcttttct taacaggtta acagtttgtg ctggttataa gaaattaacc ttcttttctt
1201 tttgccaagg gttgcatgtg aattatacct ttcttaatat ttgattaaat aatgcagtca
1261 gctaagagcc aacaaaaccc catatccaga tagttagctg tgagccagca ctcagctgtg
1321 tcagatttga ttaatattgt attaatctca tggatggttc tcaaaggtaa aaaaagagag
1381 agaaagagag agagctagct gtgggattct gtgtagttct tcactatctg ttccagggct
1441 agtcggagga tcataagcaa gctcagacct gcccccagtt cagtacttga ccacatccca
1501 ggatttatgg tgtctgtcta tggccacagc caaccagaat gagtgggtca tcttataacc
1561 tttctgaagc tccatgagga catgtataga atgctgcacc ttcagccagc tctgctctga
1621 tctttgcaga tctttaagtt gtcactgttt ctacggctag acaaatatga ctggtggtct
1681 agactaccca tagttcatcc tcctcacata acttatttga agttgtcagg gaatgtcatt
1741 acactctgat ttttaaaatt atgcagtatt ttccagttct cagagaggtc atggttgtgc
1801 ccagggaagt gtgttgcatt aactaaagct aaaattatca gaatcctatt tacataaagc
1861 atattaaaat atctgctcct taattttctg aaggaaatag atgtaagttt ctgctacttg
1921 aaacctacag aaccgacaaa aattagggtg ctaaaaaaat gacagtgaaa tctcatcgta
1981 aggcagctag ttctaatttg catttgaatg tataacaaat tgcatcactt ttcacaaact
2041 taacacctgc ctggagtaaa aatctgattt gacccctctg ctcatgtttc atcatatttg
2101 tagctctgca tcatttagca tttttatgac tttttttgct ttatataaat tattgtaaaa
2161 tccagattgt ttttaccaca tgatgacgtg actcaggatt aaatcctgag ctacagtttc
2221 aaaaggaaaa tcattttttt cttatgcaaa gatggtaaaa cactgttgtc attttttcca
2281 gtattcaata agtagccttt gtacaaattt aaaaccaaaa actattctgt tgtttttctt
2341 cacctactag ccttagtttt taaccaagta tccaaggatg tcaacttgac atccttccct
2401 tagcatgcca acaagccatt agactgattc ttccaccnac ttttatgaac ctccaggaag
2461 aaaaacactt gccaaaacat taagagaact ttgattctgc cttaagaggg tataaaacta
2521 aaccatttga aggcacagcc catnaagact atacttgatt ttgccccaag attctgactt
2581 ctggctacat ttcacctgca gttacctggg ctatcggccg tgatacattt tttaattcat
2641 ttgttttcca ccattatatt tttatacttt tcagttagat atacttctgc ataaagagta
2701 tatatacagt gtaggataaa ggcatatcgt acatggcaat gctgttaaac atggtaagac
2761 tgaggttctg caggattaaa gtcagattaa gggtacagaa agcacacctt gatactgagg
2821 ccatttagag caatttgttt aatgagtggt tcagccatca ccaccagtgg ccacctgttc
2881 aataaaattg cctagcacca cagtggagag tcatctacac cccaaggtga gctgtaagac
2941 tggccaattc tttgaagacc tttttttctg ttgggagaat ggaaagcaga aagggaagcc
3001 aggctcttta atgttccaag tcagctgtgc ctcacacaag gctctctttt tagctactaa
3061 gtaacatact caagactttg tatctgtgca gcattttatg gaaatcactg gtgacctata
3121 ggatattact aaattatttc aatagcttta tttttttcct acttggattc taggtggaca
3181 ttacagagtt gaattcctca ctaccccctc ccgctgtcat ttgttttatt ggaaaacttc
3241 atgttaacta gagtgtacaa aaagtctgtt tcctgctgag ccaatagtga tttgtttgca
3301 tatcacctaa tgtgaaaagt gctcatctgt gaactctaca gcaaattata ttttagaaaa
3361 tactttgtga ggccgggcat ggtggcagag cgagactccg tccc
//