LOCUS       AB758275                 714 bp    mRNA    linear   SYN 02-MAY-2013
DEFINITION  Synthetic construct EmGFP mRNA for green fluorescent protein,
            partial cds.
ACCESSION   AB758275
VERSION     AB758275.1
KEYWORDS    .
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Shimosato,T. and Shigemori,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2012) to the DDBJ/EMBL/GenBank databases.
            Contact:Takeshi Shimosato
            Shinshu University, Graduate School of Agriculture; 8304
            Minamiminowa-mura, Kamiina-gun, Nagano 399-4598, Japan
REFERENCE   2
  AUTHORS   Shigemori,S., Yonekura,S., Sato,T., Otani,H. and Shimosato,T.
  TITLE     Expression of the immunoreactive buckwheat major allergenic
            storage protein in Lactococcus lactis
  JOURNAL   Appl. Microbiol. Biotechnol. 97, 3603-3611 (2013)
REFERENCE   3
  AUTHORS   Shigemori,S., Yonekura,S., Sato,T., Nakanishi,M., Otani,H. and
            Shimosato,T.
  TITLE     Expression of a biologically active GFP-alpha S1-casein fusion
            protein in Lactococcus lactis
  JOURNAL   Curr. Microbiol. 64, 569-575 (2012)
  REMARK    DOI:10.1007/s00284-012-0111-x
COMMENT     
FEATURES             Location/Qualifiers
     source          1..714
                     /db_xref="taxon:32630"
                     /mol_type="mRNA"
                     /organism="synthetic construct"
     CDS             <1..>714
                     /codon_start=1
                     /gene="EmGFP"
                     /note="EmGFP optimized for lacts"
                     /product="green fluorescent protein"
                     /protein_id="BAM63381.1"
                     /transl_table=11
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNG
                     IKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITLGMDELYK"
BASE COUNT          244 a          116 c          149 g          205 t
ORIGIN      
        1 gtttctaaag gcgaagaatt gtttactggt gttgtaccca tacttgttga attagatgga
       61 gatgttaatg gacataagtt ttcagtatct ggagaaggtg aaggagatgc cacttatggg
      121 aaattgacgc ttaagtttat ctgtacaact ggaaaattac cagttccttg gcctactctt
      181 gttacgacct ttacctatgg tgtacaatgc tttgctagat atcctgatca tatgaagcaa
      241 catgacttct tcaagtcagc tatgccagaa ggttatgtgc aagaaagaac cattttcttt
      301 aaagacgatg ggaattacaa aacacgagca gaagtcaaat ttgaagggga tacattagtc
      361 aatcgtatag aactgaaagg cattgacttt aaagaggatg gcaatatttt gggtcataaa
      421 ctcgaataca actacaatag ccataaagtg tatattacag ccgataaaca gaagaatggt
      481 atcaaagtga actttaaaac tcgtcacaat attgaggatg gtagtgtaca actagctgac
      541 cattatcaac agaatacacc aattggagat ggaccagtct tattacctga taaccactat
      601 ctttcaacac aaagtgcgtt gtcaaaagat ccgaatgaga aacgcgatca tatggtttta
      661 ctagagtttg ttacagcagc aggaattact ttaggtatgg atgaactgta taaa
//