LOCUS AB451377 846 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens SIAH1 mRNA for seven in absentia homolog 1 isoform a, partial cds, clone: FLJ08065AAAF. ACCESSION AB451377 VERSION AB451377.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 846) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of the ORF. This is an F-type clone that deletes the stop codon for C-terminal tagged proteins. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..846 /clone="FLJ08065AAAF" /db_xref="H-InvDB:HIT000487590" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..>846 /codon_start=1 /gene="SIAH1" /product="seven in absentia homolog 1 isoform a" /protein_id="BAG70191.1" /transl_table=1 /translation="MSRQTATALPTGTSKCPPSQRVPALTGTNASNNDLASLFECPVC FDYVLPPILQCQSGHLVCSNCRPKLTCCPTCRGPLGSIRNLAMEKVANSVLFPCKYAS SGCEITLPHTEKADHEELCEFRPYSCPCPGASCKWQGSLDAVMPHLMHQHKSITTLQG EDIVFLATDINLPGAVDWVMMQSCFGFHFMLVLEKQEKYDGHQQFFAIVQLIGTRKQA ENFAYRLELNGHRRRLTWEATPRSIHEGIATAIMNSDCLVFDTSIAQLFAENGNLGIN VTISMC" BASE COUNT 214 a 201 c 190 g 241 t ORIGIN 1 atgagccgtc agactgctac agcattacct accggtacct cgaagtgtcc accatcccag 61 agggtgcctg ccctgactgg cacaaatgca tccaacaatg acttggcgag tctttttgag 121 tgtccagtct gctttgacta tgtgttaccg cccattcttc aatgtcagag tggccatctt 181 gtttgtagca actgtcgccc aaagctcaca tgttgtccaa cttgccgggg ccctttggga 241 tccattcgca acttggctat ggagaaagtg gctaattcag tacttttccc ctgtaaatat 301 gcgtcttctg gatgtgaaat aactctgcca cacacagaaa aagcagacca tgaagagctc 361 tgtgagttta ggccttattc ctgtccgtgc cctggtgctt cctgtaaatg gcaaggctct 421 ctggatgctg taatgcccca tctgatgcat cagcataagt ccattacaac cctacaggga 481 gaggatatag tttttcttgc tacagacatt aatcttcctg gtgctgttga ctgggtgatg 541 atgcagtcct gttttggctt tcacttcatg ttagtcttag agaaacagga aaaatacgat 601 ggtcaccagc agttcttcgc aatcgtacag ctgataggaa cacgcaagca agctgaaaat 661 tttgcttacc gacttgagct aaatggtcat aggcgacgat tgacttggga agcgactcct 721 cgatctattc atgaaggaat tgcaacagcc attatgaata gcgactgtct agtctttgac 781 accagcattg cacagctttt tgcagaaaat ggcaatttag gcatcaatgt aactatttcc 841 atgtgt //