LOCUS AB451353 1416 bp mRNA linear HUM 02-SEP-2008 DEFINITION Homo sapiens EIF2S3 mRNA for eukaryotic translation initiation factor 2 subunit 3, partial cds, clone: FLJ08020AAAF. ACCESSION AB451353 VERSION AB451353.1 KEYWORDS Gateway cloning system. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1416) AUTHORS Kawamura,Y., Nomura,N. and Goshima,N. TITLE Direct Submission JOURNAL Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases. Contact:Naoki Goshima Biomedicinal Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST); 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan REFERENCE 2 AUTHORS Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R., Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T., Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C., Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M., Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K., Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A., Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y., Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T., Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H., Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T., Imai,J.-I., Watanabe,S. and Nomura,N. TITLE Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility JOURNAL Unpublished (2008) COMMENT This human Gateway entry clone was constructed by recombining an attB-attached open reading frame (ORF) fragment with attP sequences of the Gateway donor vector pDONR201. The ORF sequence in the entry clone is flanked with attL1 sequence (100 nt) - spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) - spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the translational initiation codon (ATG) and is also flanked with spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of the ORF. This is an F-type clone that deletes the stop codon for C-terminal tagged proteins. DNA sequences of the entire ORF and flanking regions were validated by sequencing. Clone structure of the ORF and flanking sequences is as follows: 5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3' TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence. attL sequences are described in lower cases). Clone information: http://www.HGPD.jp/ This clone was produced in the "Functional Analysis of Protein and Research Application" project supported by the New Energy and Industrial Technology Development Organization (NEDO), Japan FEATURES Location/Qualifiers source 1..1416 /clone="FLJ08020AAAF" /db_xref="H-InvDB:HIT000487566" /db_xref="taxon:9606" /mol_type="mRNA" /note="Vector: pDONR201" /organism="Homo sapiens" CDS 1..>1416 /codon_start=1 /gene="EIF2S3" /product="eukaryotic translation initiation factor 2 subunit 3" /protein_id="BAG70167.1" /transl_table=1 /translation="MAGGEAGVTLGQPHLSRQDLTTLDVTKLTPLSHEVISRQATINI GTIGHVAHGKSTVVKAISGVHTVRFKNELERNITIKLGYANAKIYKLDDPSCPRPECY RSCGSSTPDEFPTDIPGTKGNFKLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIA GNESCPQPQTSEHLAAIEIMKLKHILILQNKIDLVKESQAKEQYEQILAFVQGTVAEG APIIPISAQLKYNIEVVCEYIVKKIPVPPRDFTSEPRLIVIRSFDVNKPGCEVDDLKG GVAGGSILKGVLKVGQEIEVRPGIVSKDSEGKLMCKPIFSKIVSLFAEHNDLQYAAPG GLIGVGTKIDPTLCRADRMVGQVLGAVGALPEIFTELEISYFLLRRLLGVRTEGDKKA AKVQKLSKNEVLMVNIGSLSTGGRVSAVKADLGKIVLTNPVCTEVGEKIALSRRVEKH WRLIGWGQIRRGVTIKPTVDDD" BASE COUNT 429 a 266 c 346 g 375 t ORIGIN 1 atggcgggcg gagaagctgg agtgactcta gggcagccgc atctttcgcg tcaggatctc 61 accaccttgg atgttaccaa gttgacgcca ctttcacatg aagttatcag cagacaagcc 121 acaattaaca taggtacaat tggtcatgta gctcatggga aatccacagt cgtcaaagct 181 atttctggag ttcatactgt caggttcaaa aatgaactag aaagaaatat tacaatcaag 241 cttggatatg ctaatgctaa gatttataag cttgatgacc caagttgccc tcggccagaa 301 tgttatagat cttgtgggag cagtacacct gacgagtttc ctacggacat tccagggacc 361 aaagggaact tcaaattagt cagacatgtt tcctttgttg actgtcctgg ccacgatatt 421 ttgatggcta ctatgctgaa cggtgcagca gtgatggatg cagctcttct gttgatagct 481 ggtaatgaat cttgccctca gcctcagaca tcggaacacc tggctgctat agagatcatg 541 aaactgaagc atattttgat tctacaaaat aaaattgatt tggtaaaaga aagtcaggct 601 aaagaacaat acgagcagat ccttgcattt gtccaaggta cagtagcaga gggagctccc 661 attattccaa tttcagctca gctgaaatac aatattgaag ttgtttgtga gtacatagta 721 aagaaaattc cagtaccccc aagagacttt acttcagagc cccggcttat tgttattaga 781 tcttttgatg tcaacaaacc tggctgtgaa gttgatgacc ttaagggagg tgtagctggt 841 ggtagtatcc taaaaggagt attaaaggtg ggccaggaga tagaagtaag acctggtatt 901 gtttccaaag atagtgaagg aaaactcatg tgtaaaccaa tcttttccaa aattgtatca 961 ctttttgcgg agcataatga tctgcaatat gctgctccag gcggtcttat tggagttgga 1021 acaaaaattg accccacttt gtgccgggct gacagaatgg tggggcaagt acttggtgca 1081 gtcggagctt tacctgagat attcacagaa ttggaaattt cctatttcct gcttagacgg 1141 cttctaggtg tacgcactga aggagacaag aaagcagcaa aggttcaaaa gctgtctaag 1201 aatgaagtgc tcatggtgaa cataggatcc ctgtcaacag gagggagagt tagtgctgtc 1261 aaggccgatt tgggtaaaat tgttttgacc aatccagtgt gcacagaggt aggagaaaaa 1321 attgccctta gccgaagagt tgaaaaacac tggcgtttaa ttggttgggg tcagataaga 1381 agaggagtga caatcaagcc aacagtagat gatgac //