LOCUS AB191257 3347 bp DNA circular SYN 10-JUN-2008
DEFINITION Cloning vector pGCAP1 DNA, complete sequence.
ACCESSION AB191257
VERSION AB191257.1
KEYWORDS .
SOURCE Cloning vector pGCAP1
ORGANISM Cloning vector pGCAP1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3347)
AUTHORS Kato,S.
TITLE Direct Submission
JOURNAL Submitted (27-SEP-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Seishi Kato
National Rehabilitation Center for Persons with Disabilities,
Research Institute, Department of Rehabilitation Engineering; 4-1
Namiki, Tokorozawa, Saitama 359-8555, Japan
REFERENCE 2
AUTHORS Kato,S., Ohtoko,K., Ohtake,H. and Kimura,T.
TITLE Vector-Capping: A Simple Method for Preparing a High-Quality
Full-Length cDNA Library
JOURNAL DNA Res. 12, 53-62 (2005)
COMMENT
FEATURES Location/Qualifiers
source 1..3347
/db_xref="taxon:295033"
/mol_type="other DNA"
/note="full-length cDNA cloning vector pGCAP1"
/organism="Cloning vector pGCAP1"
regulatory 31..241
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 280
/note="SV40 origin"
regulatory 550..566
/note="T7 RNA polymerase promoter"
/regulatory_class="promoter"
misc_feature 581..588
/note="SwaI site for cDNA cloning"
misc_feature 591..596
/note="KpnI site for dT tailing"
CDS complement(1823..2683)
/codon_start=1
/product="beta-lactamase"
/protein_id="BAD52433.1"
/transl_table=11
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_structure 2815..3270
/note="f1 phage IG region"
BASE COUNT 876 a 810 c 814 g 847 t
ORIGIN
1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctcgagg
361 aactgaaaaa ccagaaagtt aactggtaag tttagtcttt ttgtctttta tttcaggtcc
421 cggatccggt ggtggtgcaa atcaaagaac tgctcctcag tggatgttgc ctttacttct
481 aggcctgtac ggaagtgtta cttctgctct aaaagctgct cgagtgtaaa acgacggcca
541 gtacgtattt aatacgactc actataggga attccttaag atttaaatgt ggtaccgcgg
601 ccgcggatct ccctttagtg agggttaatt ggatccagac atgataagat acattgatga
661 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
721 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
781 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttctg cattaatgaa
841 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
901 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
961 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
1021 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
1081 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
1141 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
1201 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
1261 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
1321 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
1381 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
1441 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
1501 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
1561 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
1621 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
1681 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
1741 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
1801 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
1861 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
1921 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
1981 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
2041 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
2101 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
2161 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
2221 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
2281 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
2341 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
2401 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
2461 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
2521 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
2581 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
2641 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
2701 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
2761 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaaattg
2821 taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
2881 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
2941 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
3001 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
3061 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
3121 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
3181 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
3241 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcacacag gaaacagcta
3301 tgaccatgat cctctagagt cgacctgcag gcatgcttaa ttaaggg
//