LOCUS AB191256 3352 bp DNA circular SYN 10-JUN-2008
DEFINITION Cloning vector pKA1U5 DNA, complete sequence.
ACCESSION AB191256
VERSION AB191256.1
KEYWORDS .
SOURCE Cloning vector pKA1U5
ORGANISM Cloning vector pKA1U5
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3352)
AUTHORS Kato,S.
TITLE Direct Submission
JOURNAL Submitted (27-SEP-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Seishi Kato
National Rehabilitation Center for Persons with Disabilities,
Research Institute, Department of Rehabilitation Engineering; 4-1
Namiki, Tokorozawa, Saitama 359-8555, Japan
REFERENCE 2
AUTHORS Kato,S., Ohtoko,K., Ohtake,H. and Kimura,T.
TITLE Vector-Capping: A Simple Method for Preparing a High-Quality
Full-Length cDNA Library
JOURNAL DNA Res. 12, 53-62 (2005)
COMMENT
FEATURES Location/Qualifiers
source 1..3352
/db_xref="taxon:295032"
/mol_type="other DNA"
/note="full-length cDNA cloning vector pKA1U5"
/organism="Cloning vector pKA1U5"
regulatory 31..241
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 280
/note="SV40 origin"
regulatory 550..566
/note="T7 RNA polymerase promoter"
/regulatory_class="promoter"
misc_feature 588..593
/note="EcoRV site for cDNA cloning"
misc_feature 596..601
/note="KpnI site for dT tailing"
CDS complement(1828..2688)
/codon_start=1
/product="beta-lactamase"
/protein_id="BAD52432.1"
/transl_table=11
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_structure 2820..3275
/note="f1 phage IG region"
BASE COUNT 873 a 818 c 816 g 845 t
ORIGIN
1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctcgagg
361 aactgaaaaa ccagaaagtt aactggtaag tttagtcttt ttgtctttta tttcaggtcc
421 cggatccggt ggtggtgcaa atcaaagaac tgctcctcag tggatgttgc ctttacttct
481 aggcctgtac ggaagtgtta cttctgctct aaaagctgct cgagtgtaaa acgacggcca
541 gtacgtattt aatacgactc actataggga attccacccc cctggtggat atcctggtac
601 cgcggccgcg gatctccctt tagtgagggt taattggatc cagacatgat aagatacatt
661 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
721 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
781 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttctgcatta
841 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
901 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
961 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
1021 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
1081 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
1141 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
1201 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
1261 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
1321 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
1381 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
1441 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
1501 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
1561 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
1621 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
1681 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
1741 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
1801 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
1861 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
1921 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
1981 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
2041 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
2101 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
2161 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
2221 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
2281 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
2341 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
2401 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
2461 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
2521 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
2581 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
2641 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
2701 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
2761 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
2821 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
2881 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
2941 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
3001 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
3061 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
3121 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
3181 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
3241 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca cacaggaaac
3301 agctatgacc atgatcctct agagtcgacc tgcaggcatg cttaattaag gg
//