LOCUS AB169654 2637 bp mRNA linear PRI 06-OCT-2006 DEFINITION Macaca fascicularis brain cDNA, clone: QccE-13090, similar to human prosaposin (variant Gaucher disease and variantmetachromatic leukodystrophy) (PSAP), mRNA, RefSeq: NM_002778.1. ACCESSION AB169654 VERSION AB169654.1 KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence). SOURCE Macaca fascicularis (crab-eating macaque) ORGANISM Macaca fascicularis Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Macaca. REFERENCE 1 (bases 1 to 2637) AUTHORS Hashimoto,K., Kusuda,J. and Sugano,S. TITLE Direct Submission JOURNAL Submitted (18-MAR-2004) to the DDBJ/EMBL/GenBank databases. Contact:Katsuyuki Hashimoto National Institute of Infectious Diseases, Division of Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan URL :http://www.nih.go.jp/yoken/genebank/ REFERENCE 2 AUTHORS CONSRTM International consortium for macaque cDNA sequencing and analysis TITLE DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications JOURNAL Unpublished (2004) REFERENCE 3 AUTHORS Osada,N., Hirata,M., Tanuma,R., Kusuda,J., Hida,M., Suzuki,Y., Sugano,S., Gojobori,T., Shen,C.-K.J., Wu,C.I. and Hashimoto,K. TITLE Substitution Rate and Structural Divergence of 5'UTR Evolution: Comparative Analysis Between Human and Cynomolgus Monkey cDNAs JOURNAL Mol. Biol. Evol. 22, 1976-1982 (2005) COMMENT The International consortium for macaque cDNA sequencing and analysis consists of:Department of Virology and Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan; Division of Genetic Resources, National Institute of Infectious Diseases of Japan, Tokyo, Japan; National Health Research Institute, Taipei, Taiwan; Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & Evolution, University of Chicago, Chicago, IL, USA, Center for Information Biology, National Institute of Genetics of Japan, Mishima, Japan. Clone distribution: clone distribution information can be found at: http://www.nih.go.jp/yoken/genebank/ Lab host: TOP10 Vector: pME18S-FL3 (Acc.No. AB009864) R. Site1: DraIII (CACTGTGTG) R. Site2: DraIII (CACCATGTG) Description: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was synthesized using specific 5' and 3' primers and amplified by PCR. The PCR product was digested with SfiI and size selection was performed to exclude fragments <1.5kb.The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method. Libraries were made from: QccE: cerebellum cortex QnpA: parietal lobe QtrA: temporal lobe right QflA: frontal lobe left QmoA: medulla oblongata QbsA: brain stem QorA: occipital lobe right QtsA: testis Custom primers were used for 5' and 3'-end sequencing. The full-insert sequencing was done by primer-walking method using ABI DNA sequencer. FEATURES Location/Qualifiers source 1..2637 /clone="QccE-13090" /clone_lib="macaque cDNA library QccE" /db_xref="taxon:9541" /dev_stage="adult" /mol_type="mRNA" /organism="Macaca fascicularis" /sex="male" CDS 73..1566 /note="Homo sapiens prosaposin (variant Gaucher disease and variantmetachromatic leukodystrophy) (PSAP), mRNA, RefSeq: NM_002778.1" /protein_id="BAE01735.1" /translation="MYALFLLASLLGAALASPVLGMKECTRGSAVWCQNVKTASDCGV VKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNM SASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIP ELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTF VQALVEHVKEECDRLGPGMADMCKNYISQYSEIAIQMMMHMQDQQPKEICALVGFCDE VKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDN NKTEKEILDTFDKMCSKLPKSLSEECQEVVDTYGSSILSILLQEVSPELVCSMLRLCS GTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPY QKQCDQFVAEYEPVLIEILVEVMDPSFVFFSTCVSGRMNAQIYLTLL" BASE COUNT 573 a 669 c 754 g 641 t ORIGIN 1 aaaaagggca gatttatatc tgcggggatc agctgacgct ccgcattgca gactgcggag 61 tcaggcggcg ctatgtacgc tctcttcctc ctggccagcc tcctgggcgc ggctctagcc 121 agcccagtcc tcggaatgaa agaatgcacc agaggctcgg cagtgtggtg ccagaatgtg 181 aagacggcat ccgactgcgg ggtagtgaag cactgcctgc agaccgtttg gaacaagccg 241 acagtgaaat ctcttccttg tgacatatgc aaagacgttg tcaccgcagc tggtgatatg 301 ctgaaggaca atgccaccga ggaggagatc cttgtttact tggagaagac ctgtgactgg 361 cttccgaaac cgaacatgtc tgcttcgtgc aaggagatag tggactccta cctccctgtc 421 atcctggaca tcattaaagg agaaatgagc cgtcctgggg aggtgtgctc tgctctcaac 481 ctctgcgagt ctctccagaa gcacctggca gagctgaatc accagaagca gctggagtcc 541 aataagatcc cagagctgga catgactgag gtggtggccc ccttcatggc caacatccct 601 ctcctcctct accctcagga cggcccccgc agcaagcccc agccaaagga taatggggac 661 gtttgccagg actgcattca gatggtgacc gacatccaga ctgctgtacg gaccaactcc 721 acctttgtcc aggccttggt ggaacatgtc aaggaggagt gtgaccgcct gggccctggc 781 atggccgaca tgtgcaagaa ctatatcagc cagtattctg aaattgctat ccagatgatg 841 atgcacatgc aggatcagca acccaaggag atctgtgcgc tggttgggtt ctgtgatgag 901 gtgaaagaga tgcccatgca gactctggtc cccgccaaag tggcctccaa gaatgtcatc 961 cctgccctgg aactggtgga gcccattaag aagcacgagg tcccagcaaa gtctgatgtt 1021 tactgtgagg tgtgtgaatt cctggtgaag gaggtgacca agctgattga caacaacaag 1081 actgagaaag aaatactcga cacttttgac aaaatgtgct cgaagctgcc gaagtccctg 1141 tcggaagagt gccaggaggt ggtggacacg tacggcagct caatcctgtc catcctgctg 1201 caggaggtca gccctgagct ggtgtgcagc atgctgcgcc tctgctctgg cacgcggctg 1261 cctgcgctga ccgttcatgt gactcagcca aaggacggtg gcttctgcga agtgtgcaag 1321 aagctggtgg gttatttgga tcgtaacctg gagaaaaaca gcaccaagca ggagatcctg 1381 gctgctcttg agaaaggctg cagcttcctg cccgaccctt accagaagca gtgtgatcag 1441 tttgtggcag agtacgagcc cgtgctgatc gagatcctgg tggaggtgat ggatccttcg 1501 ttcgtgtttt tttctacttg tgtgtctggg agaatgaacg cacagatcta tttgactttg 1561 ttataaaaat agggctccct cacctccccc atttctgtgt cctttgttgt agcattgctg 1621 tctgcaaggg agcccctagc ccctggcaga catagctgct tcagtggccc ttttctctct 1681 gctagatgga tgttgatgca ctggaggtct tttagcccgc cctcgcatgg cacctgctgg 1741 aggaggagag agctctgctg gcatgagcca cagcttcttg actggaggcc atcaaccctc 1801 ttggctgggg ccttgttctg agccctgaca tgtgcttggg cactggtggg cctgggcttc 1861 tgaggtggcc tcctgccctg gtcagggacc ctcgcccctt tcctgggcct ctcagctgta 1921 caaagcagca aaacaaaggc agttttatat gaaagattag aagcccggaa taatcaggct 1981 ttttaaatga tttaattccc gctgtaatag cacagggatt ttggaagcag ctgctggtgg 2041 cttgggaccc cagtggggcc acggtttctc tgtccctggt tcaactgtga tttggctttc 2101 ccgtgtcttt cctggtgatg ccttgtttgg ggttctgtgg gtttgggtgg gaagggggcc 2161 atctgcctga atgtaacctg ctagctctcc aaaggccccg cgggcctggc ttgtgtgagc 2221 gtgtggacag tggtggccgc gctgtgcctg ctcgtgttac ctacgtgtcc ctggctgctg 2281 aggcgctgct gcagcctgca cccctccccc gtctcataga ggctcctttt gacctttctt 2341 ttcaaataaa tgtggatggc gagttcctag gcctgtggct cctgttagag ggcggcatgc 2401 tgaggggtct gctgggtgtg ggttggacgc tggggtgtgg gttggatgct gtctgcagcc 2461 cacttgggca cccatgcttc tgtctacttc tggttgccag gagacagcaa gcaaagccag 2521 caggatacgc agttgctatt aaatggactt tgtgagtttt gttttgcact aaagtttctg 2581 tgatttaaca ataaaattct gttagccaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa //