LOCUS AB168212 2172 bp mRNA linear PRI 06-OCT-2006
DEFINITION Macaca fascicularis testis cDNA clone: QtsA-10544, similar to
human transcriptional coactivator tubedown-100
(TBDN100),transcript variant 1, mRNA, RefSeq: NM_057175.2.
ACCESSION AB168212
VERSION AB168212.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Macaca fascicularis (crab-eating macaque)
ORGANISM Macaca fascicularis
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE 1 (bases 1 to 2172)
AUTHORS Hashimoto,K., Kusuda,J. and Sugano,S.
TITLE Direct Submission
JOURNAL Submitted (18-MAR-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Katsuyuki Hashimoto
National Institute of Infectious Diseases, Division of Genetic
Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
URL :http://www.nih.go.jp/yoken/genebank/
REFERENCE 2
AUTHORS
CONSRTM International consortium for macaque cDNA sequencing and analysis
TITLE DNA sequences of macaque genes expressed in brain or testis and
its evolutionary implications
JOURNAL Unpublished (2004)
REFERENCE 3
AUTHORS Osada,N., Hirata,M., Tanuma,R., Kusuda,J., Hida,M., Suzuki,Y.,
Sugano,S., Gojobori,T., Shen,C.-K.J., Wu,C.I. and Hashimoto,K.
TITLE Substitution Rate and Structural Divergence of 5'UTR Evolution:
Comparative Analysis Between Human and Cynomolgus Monkey cDNAs
JOURNAL Mol. Biol. Evol. 22, 1976-1982 (2005)
COMMENT The International consortium for macaque cDNA sequencing
and analysis consists of: Department of Virology and Human
Genome Center, Institute of Medical Science, The University of
Tokyo, Tokyo, Japan; Division of Genetic Resources, National
Institute of Infectious Diseases of Japan, Tokyo, Japan; National
Health Research Institute, Taipei, Taiwan; Institute of Molecular
Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
Evolution, University of Chicago, Chicago, IL, USA; Center for
Information Biology, National Institute of Genetics of Japan,
Mishima, Japan.
Clone distribution: clone distribution information can be
found at: http://www.nih.go.jp/yoken/genebank/
Lab host: TOP10
Vector: pME18S-FL3 (Acc.No. AB009864)
R. Site1: DraIII (CACTGTGTG)
R. Site2: DraIII (CACCATGTG)
Description: 1st strand cDNA was primed with an oligo(dT) primer
[ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
synthesized using specific 5' and 3' primers and amplified
by PCR. The PCR product was digested with SfiI and size selection
was performed to exclude fragments <1.5kb.The SfiI-digested
PCR product was cloned into distinct DraIII sites of pME18S-FL3.
XhoI sites just outside the DraIII sites can be used to
isolate the cDNA insert. Libraries were constructed by
oligo-capping method. Libraries were made from:
QccE: cerebellum cortex
QnpA: parietal lobe
QtrA: temporal lobe right
QflA: frontal lobe left
QmoA: medulla oblongata
QbsA: brain stem
QorA: occipital lobe right
QtsA: testis
Custom primers were used for 5' and 3'-end sequencing. The
full-insert sequencing was done by primer-walking method using ABI
DNA sequencer.
FEATURES Location/Qualifiers
source 1..2172
/clone="QtsA-10544"
/clone_lib="macaque cDNA library QtsA"
/db_xref="taxon:9541"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Macaca fascicularis"
/sex="male"
CDS 883..1002
/note="Homo sapiens transcriptional coactivator
tubedown-100 (TBDN100),transcript variant 1, mRNA,
RefSeq: NM_057175.2"
/protein_id="BAE00337.1"
/translation="MNIVNYSYIRIKFFGKQVSIEKLWNIFVPMKSRFVINLL"
BASE COUNT 744 a 383 c 531 g 514 t
ORIGIN
1 gtgttaggag gtgtgacccg ggtgggaaag ccctctgcgt ccgccatttt ggctgcctct
61 gtcggtctgt tcagttacca cgtgaaccgc cgacggagac ccgtagtggg ggaggcggcg
121 gcagcgttaa gtgagaaagg aaaaaagaca acgaggaaaa aggaggtgtc cgggtagggc
181 aacgcggcga cacccgaggc ctggtggtgg cggcggatcg gtatattcaa ggctgaagca
241 gctagggaac ggcagcggcg acggtcggac aaactgactg accgagccgg gtggtggcgg
301 gagcagcagg agcagccgga acgatgccgg ccgtgagcct cccgcccaag gagaatgcgc
361 tcttcaagcg gatcttgagg tgttatgaac acaaacagta tagaaatgga ttgaaattct
421 gtaaacaaat actttctaat cccaaatttg cagagcatgg agaaaccctg gctatgaaag
481 gattaacatt gaactgtttg gggaaaaagg aagaagctta tgaattggtt cgtagaggtt
541 tgagaaatga cttgaagagt catgtgtgtt ggcacgttta tggccttctt cagaggtcag
601 acaagaagta tgatgaagcc attaagtgtt acagaaatgc actaaaatgg gataaagaca
661 atcttcaaat cttaagggac ctttccttac tacagattca gatgcgagat cttgagggtt
721 atagggaaac gagataccaa ttacttcagc ttcgacctgc acagagagca tcatggattg
781 gttatgctat tgcttaccat ttattagaag attatgaaat ggcagcaaag attttagaag
841 aatttaggaa aacacaacag acatcccctg ataaggtgga ttatgaatat agtgaactac
901 tcttatatca gaatcaagtt cttcgggaag caggtctcta tagagaagct ttggaacatc
961 tttgtaccta tgaaaagcag atttgtgata aacttgctgt agaagaaacc aaaggggaac
1021 ttctgttgca actatgtcgt ttggaagatg ctgcagatgt ttacagagga ttgcaagaga
1081 gaaatcctga aaactgggcc tattacaaag gcttagaaaa agcacttaag ccagctaata
1141 tgttagaaag gctgaaaatt tacgaggagg cctggactaa gtatcccagg ggactggtgc
1201 caagaaggct gccattaaac tttttatctg gtgagaagtt taaagaatgt ttggataagt
1261 tcctaaggat gaatttcagc aagggttgcc caccagtctt caatacttta agatcattat
1321 acaaagacaa agaaaaggtg gcaatcatag aagagttagt agtaggttat gaaacttctc
1381 taaaaagctg ccggttattt aaccccaatg atgatggaaa ggaggaacca ccaaccacat
1441 tactttgggt ccagtactac ttggcacaac attatgacaa aattggtcag ccatctattg
1501 ctctggagta cataaatact gctattgaaa gtacacctac attaatagaa ctgtttctcg
1561 tgaaagctaa aatctataag cacgctggaa atattaaaga agctgcaagg tggatggatg
1621 aggcccaggc cttagacaca gcagacagat ttatcaactc caaatgtgca aaatacatgc
1681 taaaagccaa cctaattaaa gaagctgaag aaatgtgctc aaagtttaca agggaaggaa
1741 catcagcggt agagaatttg aatgaaatgc agtgcatgtg gttccaaaca gaatgtgccc
1801 aggcttataa agcaatgaat aaatttggtg aagcacttaa gaaatgtcat gagattgaga
1861 gacattttat agaaatcact gatgaccagt ttgactttca tacatactgt atgaggaaga
1921 ttacccttag atcatatgtg gacttattaa aactagaaga tgtacttcga cagcatccat
1981 tttacttcaa ggcagcaaga attgctatag aaatctattt gaagcttcat gacaaccccc
2041 ttacagatga gaataaagaa cacgaagctg atacagcaaa catgtctgac aaagagctaa
2101 agaagttacg taataaacaa agaagagctc aaaagaaagc ccagatagaa gaagagaaaa
2161 aaaaaaaaaa aa
//