LOCUS AB168212 2172 bp mRNA linear PRI 06-OCT-2006 DEFINITION Macaca fascicularis testis cDNA clone: QtsA-10544, similar to human transcriptional coactivator tubedown-100 (TBDN100),transcript variant 1, mRNA, RefSeq: NM_057175.2. ACCESSION AB168212 VERSION AB168212.1 KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence). SOURCE Macaca fascicularis (crab-eating macaque) ORGANISM Macaca fascicularis Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Macaca. REFERENCE 1 (bases 1 to 2172) AUTHORS Hashimoto,K., Kusuda,J. and Sugano,S. TITLE Direct Submission JOURNAL Submitted (18-MAR-2004) to the DDBJ/EMBL/GenBank databases. Contact:Katsuyuki Hashimoto National Institute of Infectious Diseases, Division of Genetic Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan URL :http://www.nih.go.jp/yoken/genebank/ REFERENCE 2 AUTHORS CONSRTM International consortium for macaque cDNA sequencing and analysis TITLE DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications JOURNAL Unpublished (2004) REFERENCE 3 AUTHORS Osada,N., Hirata,M., Tanuma,R., Kusuda,J., Hida,M., Suzuki,Y., Sugano,S., Gojobori,T., Shen,C.-K.J., Wu,C.I. and Hashimoto,K. TITLE Substitution Rate and Structural Divergence of 5'UTR Evolution: Comparative Analysis Between Human and Cynomolgus Monkey cDNAs JOURNAL Mol. Biol. Evol. 22, 1976-1982 (2005) COMMENT The International consortium for macaque cDNA sequencing and analysis consists of: Department of Virology and Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan; Division of Genetic Resources, National Institute of Infectious Diseases of Japan, Tokyo, Japan; National Health Research Institute, Taipei, Taiwan; Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology & Evolution, University of Chicago, Chicago, IL, USA; Center for Information Biology, National Institute of Genetics of Japan, Mishima, Japan. Clone distribution: clone distribution information can be found at: http://www.nih.go.jp/yoken/genebank/ Lab host: TOP10 Vector: pME18S-FL3 (Acc.No. AB009864) R. Site1: DraIII (CACTGTGTG) R. Site2: DraIII (CACCATGTG) Description: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was synthesized using specific 5' and 3' primers and amplified by PCR. The PCR product was digested with SfiI and size selection was performed to exclude fragments <1.5kb.The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method. Libraries were made from: QccE: cerebellum cortex QnpA: parietal lobe QtrA: temporal lobe right QflA: frontal lobe left QmoA: medulla oblongata QbsA: brain stem QorA: occipital lobe right QtsA: testis Custom primers were used for 5' and 3'-end sequencing. The full-insert sequencing was done by primer-walking method using ABI DNA sequencer. FEATURES Location/Qualifiers source 1..2172 /clone="QtsA-10544" /clone_lib="macaque cDNA library QtsA" /db_xref="taxon:9541" /dev_stage="adult" /mol_type="mRNA" /organism="Macaca fascicularis" /sex="male" CDS 883..1002 /note="Homo sapiens transcriptional coactivator tubedown-100 (TBDN100),transcript variant 1, mRNA, RefSeq: NM_057175.2" /protein_id="BAE00337.1" /translation="MNIVNYSYIRIKFFGKQVSIEKLWNIFVPMKSRFVINLL" BASE COUNT 744 a 383 c 531 g 514 t ORIGIN 1 gtgttaggag gtgtgacccg ggtgggaaag ccctctgcgt ccgccatttt ggctgcctct 61 gtcggtctgt tcagttacca cgtgaaccgc cgacggagac ccgtagtggg ggaggcggcg 121 gcagcgttaa gtgagaaagg aaaaaagaca acgaggaaaa aggaggtgtc cgggtagggc 181 aacgcggcga cacccgaggc ctggtggtgg cggcggatcg gtatattcaa ggctgaagca 241 gctagggaac ggcagcggcg acggtcggac aaactgactg accgagccgg gtggtggcgg 301 gagcagcagg agcagccgga acgatgccgg ccgtgagcct cccgcccaag gagaatgcgc 361 tcttcaagcg gatcttgagg tgttatgaac acaaacagta tagaaatgga ttgaaattct 421 gtaaacaaat actttctaat cccaaatttg cagagcatgg agaaaccctg gctatgaaag 481 gattaacatt gaactgtttg gggaaaaagg aagaagctta tgaattggtt cgtagaggtt 541 tgagaaatga cttgaagagt catgtgtgtt ggcacgttta tggccttctt cagaggtcag 601 acaagaagta tgatgaagcc attaagtgtt acagaaatgc actaaaatgg gataaagaca 661 atcttcaaat cttaagggac ctttccttac tacagattca gatgcgagat cttgagggtt 721 atagggaaac gagataccaa ttacttcagc ttcgacctgc acagagagca tcatggattg 781 gttatgctat tgcttaccat ttattagaag attatgaaat ggcagcaaag attttagaag 841 aatttaggaa aacacaacag acatcccctg ataaggtgga ttatgaatat agtgaactac 901 tcttatatca gaatcaagtt cttcgggaag caggtctcta tagagaagct ttggaacatc 961 tttgtaccta tgaaaagcag atttgtgata aacttgctgt agaagaaacc aaaggggaac 1021 ttctgttgca actatgtcgt ttggaagatg ctgcagatgt ttacagagga ttgcaagaga 1081 gaaatcctga aaactgggcc tattacaaag gcttagaaaa agcacttaag ccagctaata 1141 tgttagaaag gctgaaaatt tacgaggagg cctggactaa gtatcccagg ggactggtgc 1201 caagaaggct gccattaaac tttttatctg gtgagaagtt taaagaatgt ttggataagt 1261 tcctaaggat gaatttcagc aagggttgcc caccagtctt caatacttta agatcattat 1321 acaaagacaa agaaaaggtg gcaatcatag aagagttagt agtaggttat gaaacttctc 1381 taaaaagctg ccggttattt aaccccaatg atgatggaaa ggaggaacca ccaaccacat 1441 tactttgggt ccagtactac ttggcacaac attatgacaa aattggtcag ccatctattg 1501 ctctggagta cataaatact gctattgaaa gtacacctac attaatagaa ctgtttctcg 1561 tgaaagctaa aatctataag cacgctggaa atattaaaga agctgcaagg tggatggatg 1621 aggcccaggc cttagacaca gcagacagat ttatcaactc caaatgtgca aaatacatgc 1681 taaaagccaa cctaattaaa gaagctgaag aaatgtgctc aaagtttaca agggaaggaa 1741 catcagcggt agagaatttg aatgaaatgc agtgcatgtg gttccaaaca gaatgtgccc 1801 aggcttataa agcaatgaat aaatttggtg aagcacttaa gaaatgtcat gagattgaga 1861 gacattttat agaaatcact gatgaccagt ttgactttca tacatactgt atgaggaaga 1921 ttacccttag atcatatgtg gacttattaa aactagaaga tgtacttcga cagcatccat 1981 tttacttcaa ggcagcaaga attgctatag aaatctattt gaagcttcat gacaaccccc 2041 ttacagatga gaataaagaa cacgaagctg atacagcaaa catgtctgac aaagagctaa 2101 agaagttacg taataaacaa agaagagctc aaaagaaagc ccagatagaa gaagagaaaa 2161 aaaaaaaaaa aa //