LOCUS AA700000 465 bp mRNA linear EST 19-DEC-1997 DEFINITION zi69b06.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE:436019 3', mRNA sequence. ACCESSION AA700000 VERSION AA700000.1 DBLINK BioSample: SAMN00155062 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 465) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R. TITLE WashU-NCI human EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 457. FEATURES Location/Qualifiers source 1..465 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:1335788" /db_xref="taxon:9606" /clone="IMAGE:436019" /sex="male" /clone_lib="SAMN00155062 Soares_fetal_liver_spleen_1NFLS_S1" /dev_stage="20 week-post conception fetus" /lab_host="DH10B (ampicillin resistant)" /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Pac I; Site_2: Eco RI; This is a subtracted version of the original Soares fetal liver spleen 1NFLS library. 1st strand cDNA was primed with a Pac I - oligo(dT) primer [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Pac I and cloned into the Pac I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 154 a 79 c 96 g 136 t ORIGIN 1 atcatcattc atttatcctt ttatttagtg gcttagatcc tagaagatag attccctata 61 aaatcatttt agccacgaaa tattcataag aaaccaactt caaatgttaa tgaggatact 121 gaagtttttt ttaacctaaa aaaagtggca attttaggta accataaagc tgaggcaaag 181 gcagtttggg ttcttggaaa gaatcctgcc taccatcttc agtggcctct gggacaaagc 241 acttatgatc actgcagagg tggagcaagg taaaggactg tgtcagcagt tcatgttgtg 301 acagcagcag catctgcaga aaacgcaggt catatacctt ggcttacaca aggaatgcta 361 tgaaaaatga gccaccaggt gggagcttta acataggtaa tgattagtga aagcttctta 421 gaaagtgaga atttgtaatt tctttattaa acattacatt cagtg //