LOCUS       AA700000                 465 bp    mRNA    linear   EST 19-DEC-1997
DEFINITION  zi69b06.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA
            clone IMAGE:436019 3', mRNA sequence.
ACCESSION   AA700000
VERSION     AA700000.1
DBLINK      BioSample: SAMN00155062
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
            Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M.,
            Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F.,
            Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R.
  TITLE     WashU-NCI human EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: -40m13 fwd. ET from Amersham
            High quality sequence stop: 457.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:1335788"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:436019"
                     /sex="male"
                     /clone_lib="SAMN00155062
                     Soares_fetal_liver_spleen_1NFLS_S1"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     This is a subtracted version of the original Soares fetal
                     liver spleen 1NFLS library. 1st strand cDNA was primed
                     with a Pac I - oligo(dT) primer [5'
                     AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
BASE COUNT          154 a           79 c           96 g          136 t
ORIGIN      
        1 atcatcattc atttatcctt ttatttagtg gcttagatcc tagaagatag attccctata
       61 aaatcatttt agccacgaaa tattcataag aaaccaactt caaatgttaa tgaggatact
      121 gaagtttttt ttaacctaaa aaaagtggca attttaggta accataaagc tgaggcaaag
      181 gcagtttggg ttcttggaaa gaatcctgcc taccatcttc agtggcctct gggacaaagc
      241 acttatgatc actgcagagg tggagcaagg taaaggactg tgtcagcagt tcatgttgtg
      301 acagcagcag catctgcaga aaacgcaggt catatacctt ggcttacaca aggaatgcta
      361 tgaaaaatga gccaccaggt gggagcttta acataggtaa tgattagtga aagcttctta
      421 gaaagtgaga atttgtaatt tctttattaa acattacatt cagtg
//