LOCUS AA621632 516 bp mRNA linear EST 14-OCT-1997 DEFINITION af48c06.s1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone IMAGE:1034890 3' similar to gb:M61900 Human prostaglandin D synthase gene, complete cds. (HUMAN), mRNA sequence. ACCESSION AA621632 VERSION AA621632.1 DBLINK BioSample: SAMN00155442 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 516) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R. TITLE WashU-NCI human EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 386. FEATURES Location/Qualifiers source 1..516 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1034890" /clone_lib="SAMN00155442 Soares_total_fetus_Nb2HF8_9w" /dev_stage="8-9 weeks" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from mRNA obtained from pooled 8-9 week (total) fetus material with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo." BASE COUNT 88 a 123 c 181 g 124 t ORIGIN 1 tttttttttt ttttttactg acttgcttcc ggagtttatt gtgcaggatg aagcctgagt 61 gggcagggtt gctttggcgg ggagccgggg caggggctcg gggaaggaac agagcagaga 121 catccagagc gtgggggtca cctggctggc cgggatccca gcttcagccc tggggagtcc 181 tattgttccg tcatgcactt atcggtttgg ggcaggaaga caatggtatc ctctgtgaag 241 ccctgggcct tgcagaaggc ggtgaatttc tcctttaact cagccctggg ggtctgggtt 301 cggctgtaga gggtggccat gcggaagtcc tcgccagggc ccttgctgcc ctggctgtac 361 agcagcgcgt actggtcgta gtcggtctcc accactgaca cggagtaggt gctgccccag 421 tgggagctcc ggtagctgta ggagccgagg gacccgcggc tgaagagcat ggttccggtc 481 tcacacgggt tatctgagaa ggtgaagtca ggttga //