LOCUS       SIU02538.1               666 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 esx-
            1 secretion-associated protein espi. conserved proline
            and alanine rich protein. protein.
ACCESSION   LT708304-3952
PROTEIN_ID  SIU02538.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="espi"
                     /locus_tag="BQ2027_MB3906"
                     /note="Mb3906, -, len: 666 aa. Equivalent to Rv3876, len:
                     666 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 666 aa overlap). Conserved
                     hypothetical pro-, ala-rich protein, similar to several
                     proteins from Mycobacterium leprae e.g. Q9CDD8|ML0048
                     HYPOTHETICAL PROTEIN (586 aa), FASTA scores: opt: 1682,
                     E(): 2.1e-45, (50.75% identity in 672 aa overlap);
                     O33082|MLCB628.11c HYPOTHETICAL 52.0 KDA PROTEIN (478 aa),
                     FASTA scores: opt: 1588, E(): 1.5e-42, (53.5% identity in
                     542 aa overlap) (also has a proline rich N-terminus); etc.
                     Also similar to other proteins from Mycobacterium
                     tuberculosis, especially in C-terminus, e.g.
                     O06396|Rv0530|MTCY25D10.09 (405 aa), FASTA scores: opt:
                     670, E(): 2.5e-14, (34.85% identity in 396 aa overlap)
                     (also has Pro-rich N-terminus); etc. Note that N-terminus
                     is repetitive and highly Proline rich. Protein product
                     from Mb3906 detected using shotgun mass spectrometry and
                     SWATH mass spectrometry. Mb3906 found to be expressed
                     during exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="InterPro:IPR002586"
                     /db_xref="InterPro:IPR027417"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y5I8"
BEGIN
        1 MAADYDKLFR PHEGMEAPDD MAAQPFFDPS ASFPPAPASA NLPKPNGQTP PPTSDDLSER
       61 FVSAPPPPPP PPPPPPPTPM PIAAGEPPSP EPAASKPPTP PMPIAGPEPA PPKPPTPPMP
      121 IAGPEPAPPK PPTPPMPIAG PAPTPTESQL APPRPPTPQT PTGAPQQPES PAPHVPSHGP
      181 HQPRRTAPAP PWAKMPIGEP PPAPSRPSAS PAEPPTRPAP QHSRRARRGH RYRTDTERNV
      241 GKVATGPSIQ ARLRAEEASG AQLAPGTEPS PAPLGQPRSY LAPPTRPAPT EPPPSPSPQR
      301 NSGRRAERRV HPDLAAQHAA AQPDSITAAT TGGRRRKRAA PDLDATQKSL RPAAKGPKVK
      361 KVKPQKPKAT KPPKVVSQRG WRHWVHALTR INLGLSPDEK YELDLHARVR RNPRGSYQIA
      421 VVGLKGGAGK TTLTAALGST LAQVRADRIL ALDADPGAGN LADRVGRQSG ATIADVLAEK
      481 ELSHYNDIRA HTSVNAVNLE VLPAPEYSSA QRALSDADWH FIADPASRFY NLVLADCGAG
      541 FFDPLTRGVL STVSGVVVVA SVSIDGAQQA SVALDWLRNN GYQDLASRAC VVINHIMPGE
      601 PNVAVKDLVR HFEQQVQPGR VVVMPWDRHI AAGTEISLDL LDPIYKRKVL ELAAALSDDF
      661 ERAGRR
//