LOCUS SIU02538.1 666 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 esx- 1 secretion-associated protein espi. conserved proline and alanine rich protein. protein. ACCESSION LT708304-3952 PROTEIN_ID SIU02538.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="espi" /locus_tag="BQ2027_MB3906" /note="Mb3906, -, len: 666 aa. Equivalent to Rv3876, len: 666 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 666 aa overlap). Conserved hypothetical pro-, ala-rich protein, similar to several proteins from Mycobacterium leprae e.g. Q9CDD8|ML0048 HYPOTHETICAL PROTEIN (586 aa), FASTA scores: opt: 1682, E(): 2.1e-45, (50.75% identity in 672 aa overlap); O33082|MLCB628.11c HYPOTHETICAL 52.0 KDA PROTEIN (478 aa), FASTA scores: opt: 1588, E(): 1.5e-42, (53.5% identity in 542 aa overlap) (also has a proline rich N-terminus); etc. Also similar to other proteins from Mycobacterium tuberculosis, especially in C-terminus, e.g. O06396|Rv0530|MTCY25D10.09 (405 aa), FASTA scores: opt: 670, E(): 2.5e-14, (34.85% identity in 396 aa overlap) (also has Pro-rich N-terminus); etc. Note that N-terminus is repetitive and highly Proline rich. Protein product from Mb3906 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3906 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR002586" /db_xref="InterPro:IPR027417" /db_xref="UniProtKB/TrEMBL:A0A1R3Y5I8" BEGIN 1 MAADYDKLFR PHEGMEAPDD MAAQPFFDPS ASFPPAPASA NLPKPNGQTP PPTSDDLSER 61 FVSAPPPPPP PPPPPPPTPM PIAAGEPPSP EPAASKPPTP PMPIAGPEPA PPKPPTPPMP 121 IAGPEPAPPK PPTPPMPIAG PAPTPTESQL APPRPPTPQT PTGAPQQPES PAPHVPSHGP 181 HQPRRTAPAP PWAKMPIGEP PPAPSRPSAS PAEPPTRPAP QHSRRARRGH RYRTDTERNV 241 GKVATGPSIQ ARLRAEEASG AQLAPGTEPS PAPLGQPRSY LAPPTRPAPT EPPPSPSPQR 301 NSGRRAERRV HPDLAAQHAA AQPDSITAAT TGGRRRKRAA PDLDATQKSL RPAAKGPKVK 361 KVKPQKPKAT KPPKVVSQRG WRHWVHALTR INLGLSPDEK YELDLHARVR RNPRGSYQIA 421 VVGLKGGAGK TTLTAALGST LAQVRADRIL ALDADPGAGN LADRVGRQSG ATIADVLAEK 481 ELSHYNDIRA HTSVNAVNLE VLPAPEYSSA QRALSDADWH FIADPASRFY NLVLADCGAG 541 FFDPLTRGVL STVSGVVVVA SVSIDGAQQA SVALDWLRNN GYQDLASRAC VVINHIMPGE 601 PNVAVKDLVR HFEQQVQPGR VVVMPWDRHI AAGTEISLDL LDPIYKRKVL ELAAALSDDF 661 ERAGRR //