LOCUS SIU02335.1 810 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
BIFUNCTIONAL MEMBRANE-ASSOCIATED PENICILLIN-BINDING PROTEIN
1A/1B PONA2 (MUREIN POLYMERASE) [INCLUDES: PENICILLIN-
INSENSITIVE TRANSGLYCOSYLASE (PEPTIDOGLYCAN TGASE) + PENICILLIN-
SENSITIVE TRANSPEPTIDASE (DD-TRANSPEPTIDASE)] protein.
ACCESSION LT708304-3749
PROTEIN_ID SIU02335.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="ponA2"
/locus_tag="BQ2027_MB3707"
/note="Mb3707, ponA2, len: 810 aa. Equivalent to Rv3682,
len: 810 aa, from Mycobacterium tuberculosis strain H37Rv,
(100.0% identity in 810 aa overlap). Probable ponA2,
penicillin-binding protein (class A), bienzymatic
membrane-associated protein with transglycosylase (EC
2.4.2.-) and transpeptidase (EC 3.4.-.-) activities.
Almost identical to Q9CB85|PON1|ML2308 PENICILLIN BINDING
PROTEIN (CLASS A) from Mycobacterium leprae (803 aa) FASTA
scores: opt: 4743, E(): 3.3e-217, (87.7% identity in 806
aa overlap); or P72351|PON1|PBP1 HIGH-MOLECULAR-MASS CLASS
A PENICILLIN BINDING PROTEIN from Mycobacterium leprae
Cosmid B577 (821 aa), FASTA scores: opt: 4547, E():
6.3e-208, (88.05% identity in 769 aa overlap) (see first
citation below). Also similar to others e.g.
Q9XA34|SCH17.14 from Streptomyces coelicolor (428 aa;
fragment), FASTA scores: opt: 727, E(): 2.3e-27, (36.55%
identity in 413 aa overlap); Q9F9V7|PONA from
Mycobacterium smegmatis (715 aa), FASTA scores: opt: 446,
E(): 6.6e-14, (27.65% identity in 771 aa overlap) (see
second citation below); Q9CCY4|PONA|ML2688 from
Mycobacterium leprae (708 aa), FASTA scores: opt: 413,
E(): 2.4e-12, (26.8% identity in 660 aa overlap);
Q9X6W0|PONB|MRCB|PA4700 from Pseudomonas aeruginosa (774
aa), FASTA scores: opt: 398, E(): 1.3e-11, (27.2% identity
in 666 aa overlap); P45345|PBPB_HAEIN|MRCB|PONB|HI1725
(781 aa), FASTA scores: opt: 380, E(): 9.4e-11, (28.6%
identity in 601 aa overlap); etc. Also similar to
P71707|PONA1|Rv0050|MTCY21.13 PROBABLE BIFUNCTIONAL
PENICILLIN-BINDING PROTEIN 1A/1B (PBP1) from M.
tuberculosis (678 aa) FASTA scores: opt: 372, E(): 2e-10,
(28.35% identity in 769 aa overlap). SEEMS TO BELONG TO
THE TRANSGLYCOSYLASE FAMILY IN THE N-TERMINAL SECTION, AND
TO THE TRANSPEPTIDASE FAMILY IN THE C-TERMINAL SECTION.
Protein product from Mb3707 detected using shotgun mass
spectrometry and SWATH mass spectrometry. Mb3707 found to
be expressed during exponential growth in Sauton's minimal
media by RNA-sequencing."
/db_xref="GOA:A0A1R3Y6G6"
/db_xref="InterPro:IPR001264"
/db_xref="InterPro:IPR001460"
/db_xref="InterPro:IPR005543"
/db_xref="InterPro:IPR012338"
/db_xref="InterPro:IPR023346"
/db_xref="InterPro:IPR036950"
/db_xref="UniProtKB/TrEMBL:A0A1R3Y6G6"
BEGIN
1 MPERLPAAIT VLKLAGCCLL ASVVATALTF PFAGGLGLMS NRASEVVANG SAQLLEGQVP
61 AVSTMVDAKG NTIAWLYSQR RFEVPSDKIA NTMKLAIVSI EDKRFADHSG VDWKGTLTGL
121 AGYASGDLDT RGGSTLEQQY VKNYQLLVTA QTDAEKRAAV ETTPARKLRE IRMALTLDKT
181 FTKSEILTRY LNLVSFGNNS FGVQDAAQTY FGINASDLNW QQAALLAGMV QSTSTLNPYT
241 NPDGALARRN VVLDTMIENL PGEAEALRAA KAEPLGVLPQ PNELPRGCIA AGDRAFFCDY
301 VQEYLSRAGI SKEQVATGGY LIRTTLDPEV QAPVKAAIDK YASPNLAGIS SVMSVIKPGK
361 DAHKVLAMAS NRKYGLDLEA GETMRPQPFS LVGDGAGSIF KIFTTAAALD MGMGINAQLD
421 VPPRFQAKGL GSGGAKGCPK ETWCVVNAGN YRGSMNVTDA LATSPNTAFA KLISQVGVGR
481 AVDMAIKLGL RSYANPGTAR DYNPDSNESL ADFVKRQNLG SFTLGPIELN ALELSNVAAT
541 LASGGVWCPP NPIDQLIDRN GNEVAVTTET CDQVVPAGLA NTLANAMSKD AVGSGTAAGS
601 AGAAGWDLPM SGKTGTTEAH RSAGFVGFTN RYAAANYIYD DSSSPTDLCS GPLRHCGSGD
661 LYGGNEPSRT WFAAMKPIAN NFGEVQLPPT DPRYVDGAPG SRVPSVAGLD VDAARQRLKD
721 AGFQVADQTN SVNSSAKYGE VVGTSPSGQT IPGSIVTIQI SNGIPPAPPP PPLPEDGGPP
781 PPVGSQVVEI PGLPPITIPL LAPPPPAPPP
//