LOCUS SIU02335.1 810 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE BIFUNCTIONAL MEMBRANE-ASSOCIATED PENICILLIN-BINDING PROTEIN 1A/1B PONA2 (MUREIN POLYMERASE) [INCLUDES: PENICILLIN- INSENSITIVE TRANSGLYCOSYLASE (PEPTIDOGLYCAN TGASE) + PENICILLIN- SENSITIVE TRANSPEPTIDASE (DD-TRANSPEPTIDASE)] protein. ACCESSION LT708304-3749 PROTEIN_ID SIU02335.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ponA2" /locus_tag="BQ2027_MB3707" /note="Mb3707, ponA2, len: 810 aa. Equivalent to Rv3682, len: 810 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 810 aa overlap). Probable ponA2, penicillin-binding protein (class A), bienzymatic membrane-associated protein with transglycosylase (EC 2.4.2.-) and transpeptidase (EC 3.4.-.-) activities. Almost identical to Q9CB85|PON1|ML2308 PENICILLIN BINDING PROTEIN (CLASS A) from Mycobacterium leprae (803 aa) FASTA scores: opt: 4743, E(): 3.3e-217, (87.7% identity in 806 aa overlap); or P72351|PON1|PBP1 HIGH-MOLECULAR-MASS CLASS A PENICILLIN BINDING PROTEIN from Mycobacterium leprae Cosmid B577 (821 aa), FASTA scores: opt: 4547, E(): 6.3e-208, (88.05% identity in 769 aa overlap) (see first citation below). Also similar to others e.g. Q9XA34|SCH17.14 from Streptomyces coelicolor (428 aa; fragment), FASTA scores: opt: 727, E(): 2.3e-27, (36.55% identity in 413 aa overlap); Q9F9V7|PONA from Mycobacterium smegmatis (715 aa), FASTA scores: opt: 446, E(): 6.6e-14, (27.65% identity in 771 aa overlap) (see second citation below); Q9CCY4|PONA|ML2688 from Mycobacterium leprae (708 aa), FASTA scores: opt: 413, E(): 2.4e-12, (26.8% identity in 660 aa overlap); Q9X6W0|PONB|MRCB|PA4700 from Pseudomonas aeruginosa (774 aa), FASTA scores: opt: 398, E(): 1.3e-11, (27.2% identity in 666 aa overlap); P45345|PBPB_HAEIN|MRCB|PONB|HI1725 (781 aa), FASTA scores: opt: 380, E(): 9.4e-11, (28.6% identity in 601 aa overlap); etc. Also similar to P71707|PONA1|Rv0050|MTCY21.13 PROBABLE BIFUNCTIONAL PENICILLIN-BINDING PROTEIN 1A/1B (PBP1) from M. tuberculosis (678 aa) FASTA scores: opt: 372, E(): 2e-10, (28.35% identity in 769 aa overlap). SEEMS TO BELONG TO THE TRANSGLYCOSYLASE FAMILY IN THE N-TERMINAL SECTION, AND TO THE TRANSPEPTIDASE FAMILY IN THE C-TERMINAL SECTION. Protein product from Mb3707 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3707 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y6G6" /db_xref="InterPro:IPR001264" /db_xref="InterPro:IPR001460" /db_xref="InterPro:IPR005543" /db_xref="InterPro:IPR012338" /db_xref="InterPro:IPR023346" /db_xref="InterPro:IPR036950" /db_xref="UniProtKB/TrEMBL:A0A1R3Y6G6" BEGIN 1 MPERLPAAIT VLKLAGCCLL ASVVATALTF PFAGGLGLMS NRASEVVANG SAQLLEGQVP 61 AVSTMVDAKG NTIAWLYSQR RFEVPSDKIA NTMKLAIVSI EDKRFADHSG VDWKGTLTGL 121 AGYASGDLDT RGGSTLEQQY VKNYQLLVTA QTDAEKRAAV ETTPARKLRE IRMALTLDKT 181 FTKSEILTRY LNLVSFGNNS FGVQDAAQTY FGINASDLNW QQAALLAGMV QSTSTLNPYT 241 NPDGALARRN VVLDTMIENL PGEAEALRAA KAEPLGVLPQ PNELPRGCIA AGDRAFFCDY 301 VQEYLSRAGI SKEQVATGGY LIRTTLDPEV QAPVKAAIDK YASPNLAGIS SVMSVIKPGK 361 DAHKVLAMAS NRKYGLDLEA GETMRPQPFS LVGDGAGSIF KIFTTAAALD MGMGINAQLD 421 VPPRFQAKGL GSGGAKGCPK ETWCVVNAGN YRGSMNVTDA LATSPNTAFA KLISQVGVGR 481 AVDMAIKLGL RSYANPGTAR DYNPDSNESL ADFVKRQNLG SFTLGPIELN ALELSNVAAT 541 LASGGVWCPP NPIDQLIDRN GNEVAVTTET CDQVVPAGLA NTLANAMSKD AVGSGTAAGS 601 AGAAGWDLPM SGKTGTTEAH RSAGFVGFTN RYAAANYIYD DSSSPTDLCS GPLRHCGSGD 661 LYGGNEPSRT WFAAMKPIAN NFGEVQLPPT DPRYVDGAPG SRVPSVAGLD VDAARQRLKD 721 AGFQVADQTN SVNSSAKYGE VVGTSPSGQT IPGSIVTIQI SNGIPPAPPP PPLPEDGGPP 781 PPVGSQVVEI PGLPPITIPL LAPPPPAPPP //