LOCUS SIU01593.1 2112 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable
polyketide synthase pks15 protein.
ACCESSION LT708304-3007
PROTEIN_ID SIU01593.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="pks1"
/locus_tag="BQ2027_MB2971C"
/note="Mb2971c, pks1, len: 2112 aa. Equivalent to Rv2947c
and Rv2946c, len: 496 aa and 1616 aa, from Mycobacterium
tuberculosis strain H37Rv, (99.6% identity in 488 aa
overlap and 99.9% identity in 1616 aa overlap). Rv2947c:
Probable pks15, polyketide synthase. Almost identical to
G560508|Q50469 PKS002B protein from Mycobacterium
tuberculosis (495 aa), FASTA scores: opt: 3270, E(): 0,
(99.6% identity in 496 a a overlap). Similar to
Mycobacterium tuberculosis proteins
MTCY338.20|RV2931|PPSA_MYCTU ppsA phenolpthiocerol
synthesis (1876 aa) (49.9% identity in 465 aa overlap);
MTCY24G1.09|RV2940C|P96291 Putative mas, mycocerosic acid
synthase (2111 aa) (50.2% identity in 454 aa overlap); and
MTCY22H8.03|RV2382C|P71718 hypothetical protein (444 aa)
(47.6% identity in 437 aa overlap). Contains PS00606
Beta-ketoacyl synthases active site. Rv2946c: Probable
pks1, polyketide synthase, similar to many e.g.
ML035|AL583917|Q9CD81 putative polyketide synthase from
Mycobacterium leprae (2103 aa), Fasta scores: opt: 8761,
E(): 0, (82.6% identity in 1620 aa overlap); etc. Almost
identical in part to G560507|Q50470 PKS002C protein from
Mycobacterium tuberculosis (fragment) (950 aa), Fasta
scores: opt: 5685, E(): 0, (95.3% identity in 927 aa
overlap). Also similar to Mycobacterium tuberculosis
polyketide synthases pks7|Rv1661|P94996 (2126 aa) (54.6%
identity in 1632 aa); pks12|Rv2048c|O53490 (4151 aa)
(58.0% identity in 1606 aa); pks8|rv1662|O65933 (1602 aa)
(59.7% identity in 1144 aa). Contains a PS00012
Phosphopantetheine attachment site.
REMARK-M.bovis-M.tuberculosis: In Mycobacterium
tuberculosis H37Rv, pks1 and pks15 exist as 2 genes. In
Mycobacterium bovis, a single base insertion (*-g) results
in a single product that is more similar to pks1. Protein
product from Mb2971c detected using shotgun mass
spectrometry and SWATH mass spectrometry. Mb2971c found to
be expressed during exponential growth in Sauton's minimal
media by RNA-sequencing (TPM > 20)."
/db_xref="GOA:Q7TXK8"
/db_xref="InterPro:IPR001227"
/db_xref="InterPro:IPR006162"
/db_xref="InterPro:IPR009081"
/db_xref="InterPro:IPR011032"
/db_xref="InterPro:IPR013154"
/db_xref="InterPro:IPR013968"
/db_xref="InterPro:IPR014030"
/db_xref="InterPro:IPR014031"
/db_xref="InterPro:IPR014043"
/db_xref="InterPro:IPR015083"
/db_xref="InterPro:IPR016035"
/db_xref="InterPro:IPR016036"
/db_xref="InterPro:IPR016039"
/db_xref="InterPro:IPR018201"
/db_xref="InterPro:IPR020801"
/db_xref="InterPro:IPR020806"
/db_xref="InterPro:IPR020807"
/db_xref="InterPro:IPR020841"
/db_xref="InterPro:IPR020843"
/db_xref="InterPro:IPR032821"
/db_xref="InterPro:IPR036291"
/db_xref="InterPro:IPR036299"
/db_xref="InterPro:IPR036736"
/db_xref="InterPro:IPR042104"
/db_xref="UniProtKB/Swiss-Prot:Q7TXK8"
BEGIN
1 MIEEQRTMSV EGADQQSEKL FHYLKKVAVE LDETRARLRE YEQRATEPVA VVGIGCRFPG
61 GVDGPDGLWD VVSAGRDVVS EFPTDRGWDV EGLYDPDPDA EGKTYTRWGA FLDDATGFDA
121 GFFGIAPSEV LAMDPQQRLM LEVSWEALEH AGIDPLSLRG SATGVYTGIF AASYGNRDTG
181 GLQGYGLTGT SISVASGRVS YVLGLQGPAV SVDTACSSSL VAIHWAMSSL RSGECDLALA
241 GGVTVMGLPS IFVGFSRQRG LAADGRCKAF AAAADGTGWG EGAGVVVLER LSDARRLGHS
301 VLAVVRGSAV NQDGASNGLT APNGLAQQRV IQAALANAGL SAADVDVVEA HGTATTLGDP
361 IEAQALLSTY GQGRPAEQPL WVGSIKSNMG HTQAAAGVAG VIKMVQAMRH GVMPATLHVD
421 EPSPRVDWTS GAVSVLTEAR EWSVDGRPRR AAVSSFGISG TNAHLILEEA PVPAPAEAPV
481 EASESTGGPR PSMVPWVISA RSAEALTAQA GRLMAHVQAN PGLDPIDVGC SLASRSVFEH
541 RAVVVGASRE QLIAGLAGLA AGEPGAGVAV GQPGSVGKTV VVFPGQGAQR IGMGRELYGE
601 LPVFAQAFDA VADELDRHLR LPLRDVIWGA DADLLDSTEF AQPALFAVEV ASFAVLRDWG
661 VLPDFVMGHS VGELAAAHAA GVLTLADAAM LVVARGRLMQ ALPAGGAMVA VAASEDEVEP
721 LLGEGVGIAA INAPESVVIS GAQAAANAIA DRFAAQGRRV HQLAVSHAFH SPLMEPMLEE
781 FARVAARVQA REPQLGLVSN VTGELAGPDF GSAQYWVDHV RRPVRFADSA RHLQTLGATH
841 FIEAGPGSGL TGSIEQSLAP AEAMVVSMLG KDRPELASAL GAAGQVFTTG VPVQWSAVFA
901 GSGGRRVQLP TYAFQRRRFW ETPGADGPAD AAGLGLGATE HALLGAVVER PDSDEVVLTG
961 RLSLADQPWL ADHVVNGVVL FPGAGFVELV IRAGDEVGCA LIEELVLAAP LVMHPGVGVQ
1021 VQVVVGAADE SGHRAVSVYS RGDQSQGWLL NAEGMLGVAA AETPMDLSVW PPEGAESVDI
1081 SDGYAQLAER GYAYGPAFQG LVAIWRRGSE LFAEVVAPGE AGVAVDRMGM HPAVLDAVLH
1141 ALGLAVEKTQ ASTETRLPFC WRGVSLHAGG AGRVRARFAS AGADAISVDV CDATGLPVLT
1201 VRSLVTRPIT AEQLRAAVTA AGGASDQGPL EVVWSPISVV SGGANGSAPP APVSWADFCA
1261 GSDGDASVVV WELESAGGQA SSVVGSVYAA THTALEVLQS WLGADRAATL VVLTHGGVGL
1321 AGEDISDLAA AAVWGMARSA QAENPGRIVL IDTDAAVDAS VLAGVGEPQL LVRGGTVHAP
1381 RLSPAPALLA LPAAESAWRL AAGGGGTLED LVIQPCPEVQ APLQAGQVRV AVAAVGVNFR
1441 DVVAALGMYP GQAPPLGAEG AGVVLETGPE VTDLAVGDAV MGFLGGAGPL AVVDQQLVTR
1501 VPQGWSFAQA AAVPVVFLTA WYGLADLAEI KAGESVLIHA GTGGVGMAAV QLARQWGVEV
1561 FVTASRGKWD TLRAMGFDDD HIGDSRTCEF EEKFLAVTEG RGVDVVLDSL AGEFVDASLR
1621 LLVRGGRFLE MGKTDIRDAQ EIAANYPGVQ YRAFDLSEAG PARMQEMLAE VRELFDTREL
1681 HRLPVTTWDV RCAPAAFRFM SQARHIGKVV LTMPSALADR LADGTVVITG ATGAVGGVLA
1741 RHLVGAYGVR HLVLASRRGD RAEGAAELAA DLTEAGAKGQ VVACDVADRA AVAGLFAQLS
1801 REYPPVRGVI HAAGVLDDAV ITSLTPDRID TVLRAKVDAA WNLHQATSDL DLSMFVLCSS
1861 IAATVGSPGQ GNYSAANAFL DGLAAHRQAA GLAGISLAWG LWEQPGGMTA HLSSRDLARM
1921 SRSGLAPMSP AEAVELFDAA LAIDHPLAVA TLLDRAALDA RAQAGALPAL FSGLARRPRR
1981 RQIDDTGDAT SSKSALAQRL HGLAADEQLE LLVGLVCLQA AAVLGRPSAE DVDPDTEFGD
2041 LGFDSLTAVE LRNRLKTATG LTLPPTVIFD HPTPTAVAEY VAQQMSGSRP TESGDPTSQV
2101 VEPAAAEVSV HA
//