LOCUS DAA09136.1 316 aa PRT PLN 23-JAN-2024 DEFINITION Saccharomyces cerevisiae S288C bifunctional protein farnesyltransfer ase/protein geranylgeranyltransferase protein. ACCESSION BK006944-195 PROTEIN_ID DAA09136.1 SOURCE Saccharomyces cerevisiae S288C ORGANISM Saccharomyces cerevisiae S288C Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. REFERENCE 1 (bases 1 to 666816) AUTHORS Dujon,B., Alexandraki,D., Andre,B., Ansorge,W., Baladron,V., Ballesta,J.P., Banrevi,A., Bolle,P.A., Bolotin-Fukuhara,M., Bossier,P. et al. TITLE Complete DNA sequence of yeast chromosome XI JOURNAL Nature 369 (6479), 371-378 (1994) PUBMED 8196765 REFERENCE 2 (bases 1 to 666816) AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B., Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M., Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and Oliver,S.G. TITLE Life with 6000 genes JOURNAL Science 274 (5287), 546 (1996) PUBMED 8849441 REFERENCE 3 (bases 1 to 666816) AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M., Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S., Weng,S. and Cherry,J.M. TITLE New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources JOURNAL Genetics 220 (4) (2022) PUBMED 34897464 REFERENCE 4 (bases 1 to 666816) CONSRTM Saccharomyces Genome Database TITLE Direct Submission JOURNAL Submitted (14-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA REFERENCE 5 (bases 1 to 666816) CONSRTM Saccharomyces Genome Database TITLE Direct Submission JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA REMARK Sequence update by submitter REFERENCE 6 (bases 1 to 666816) CONSRTM Saccharomyces Genome Database TITLE Direct Submission JOURNAL Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA REMARK Protein update by submitter COMMENT THIRD PARTY DATABASE: This TPA record uses data from DDBJ/EMBL/GenBank entries JF740677.1, Z28001.1, Z28002.1, Z28003.1, Z28004.1, Z28005.1, Z28006.1, Z28007.1, Z28008.1, Z28009.1, Z28010.1, Z28011.1, Z28012.1, Z28013.1, Z28014.1, Z28015.1, Z28016.1, Z28017.1, Z28018.1, Z28019.1, Z28020.1, Z28021.1, Z28022.1, Z28023.1, Z28024.1, Z28025.1, Z28026.1, Z28027.1, Z28028.1, Z28029.1, Z28031.1, Z28032.1, Z28033.1, Z28034.1, Z28035.1, Z28038.1, Z28039.1, Z28040.1, Z28041.1, Z28042.1, Z28043.1, Z28044.1, Z28045.1, Z28046.1, Z28047.1, Z28048.1, Z28049.1, Z28050.1, Z28051.1, Z28052.1, Z28054.1, Z28055.1, Z28056.1, Z28057.1, Z28058.1, Z28059.1, Z28060.1, Z28061.1, Z28062.1, Z28063.1, Z28064.1, Z28065.1, Z28066.1, Z28067.1, Z28068.1, Z28069.1, Z28070.1, Z28071.1, Z28072.1, Z28073.1, Z28074.1, Z28075.1, Z28077.1, Z28078.1, Z28079.1, Z28080.1, Z28081.1, Z28082.1, Z28084.1, Z28085.1, Z28086.1, Z28087.1, Z28088.1, Z28089.1, Z28090.1, Z28091.1, Z28092.1, Z28093.1, Z28094.1, Z28095.1, Z28096.1, Z28097.1, Z28098.1, Z28099.1, Z28100.1, Z28101.1, Z28102.1, Z28103.1, Z28104.1, Z28105.1, Z28106.1, Z28107.1, Z28108.1, Z28109.1, Z28110.1, Z28111.1, Z28113.1, Z28114.1, Z28115.1, Z28117.1, Z28118.1, Z28120.1, Z28121.1, Z28122.1, Z28123.1, Z28125.1, Z28126.1, Z28127.1, Z28128.1, Z28129.1, Z28130.1, Z28131.1, Z28133.1, Z28134.1, Z28135.1, Z28137.1, Z28138.1, Z28139.1, Z28140.1, Z28141.1, Z28142.1, Z28143.1, Z28144.1, Z28145.1, Z28146.1, Z28148.1, Z28149.1, Z28150.1, Z28151.1, Z28152.1, Z28154.1, Z28155.1, Z28156.1, Z28157.1, Z28158.1, Z28159.1, Z28160.1, Z28161.1, Z28162.1, Z28163.1, Z28164.1, Z28165.1, Z28166.1, Z28167.1, Z28168.1, Z28169.1, Z28171.1, Z28172.1, Z28173.1, Z28174.1, Z28175.1, Z28176.1, Z28177.1, Z28179.1, Z28180.1, Z28181.1, Z28182.1, Z28183.1, Z28184.1, Z28185.1, Z28186.1, Z28187.1, Z28188.1, Z28189.1, Z28190.1, Z28191.1, Z28192.1, Z28193.1, Z28194.1, Z28195.1, Z28196.1, Z28197.1, Z28198.1, Z28200.1, Z28201.1, Z28202.1, Z28203.1, Z28204.1, Z28205.1, Z28206.1, Z28207.1, Z28208.1, Z28209.1, Z28210.1, Z28211.1, Z28212.1, Z28213.1, Z28214.1, Z28215.1, Z28216.1, Z28217.1, Z28218.1, Z28219.1, Z28220.1, Z28221.1, Z28222.1, Z28223.1, Z28224.1, Z28225.1, Z28226.1, Z28227.1, Z28228.1, Z28229.1, Z28230.1, Z28231.1, Z28232.1, Z28233.1, Z28234.1, Z28235.1, Z28236.1, Z28237.1, Z28238.1, Z28239.1, Z28240.1, Z28241.1, Z28242.1, Z28243.1, Z28244.1, Z28245.1, Z28246.1, Z28247.1, Z28248.1, Z28249.1, Z28250.1, Z28251.1, Z28252.1, Z28253.1, Z28254.1, Z28255.1, Z28256.1, Z28257.1, Z28258.1, Z28259.1, Z28260.1, Z28261.1, Z28262.1, Z28263.1, Z28264.1, Z28265.1, Z28266.1, Z28267.1, Z28268.1, Z28269.1, Z28270.1, Z28271.1, Z28272.1, Z28273.1, Z28274.1, Z28275.1, Z28276.1, Z28277.1, Z28278.1, Z28279.1, Z28280.1, Z28281.1, Z28282.1, Z28283.1, Z28284.1, Z28285.1, Z28286.1, Z28287.1, Z28288.1, Z28289.1, Z28290.1, Z28291.1, Z28292.1, Z28293.1, Z28294.1, Z28295.1, Z28296.1, Z28297.1, Z28298.1, Z28299.1, Z28300.1, Z28301.1, Z28302.1, Z28303.1, Z28304.1, Z28305.1, Z28306.1, Z28307.1, Z28308.1, Z28309.1, Z28310.1, Z28311.1, Z28312.1, Z28313.1, Z28314.1, Z28315.1, Z28316.1, Z28317.1, Z28318.1, Z28319.1, Z28320.1, Z28321.1, Z28322.1, Z28323.1, Z28324.1, Z28325.1, Z28326.1, Z28327.1, Z28328.1, Z28329.1 and Z28330.1 On Apr 18, 2011 this sequence version replaced BK006944.1. Chromosome XI sequence was verified by resequencing. The sequence was mapped to the previous GenBank records except for a 500 bp region. That region was determined by sequencing a descendant of the S288C strain, AB972, and is present in GenBank as JF740677. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-4-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Saccharomyces cerevisiae S288C" /mol_type="genomic DNA" /strain="S288C" /db_xref="taxon:559292" /chromosome="XI" protein /gene="RAM2" /locus_tag="YKL019W" /EC_number="2.5.1.58" /EC_number="2.5.1.59" /experiment="EXISTENCE:direct assay:GO:0004660 protein farnesyltransferase activity [PMID:1763050]" /experiment="EXISTENCE:direct assay:GO:0004661 protein geranylgeranyltransferase activity [PMID:2034682]" /experiment="EXISTENCE:direct assay:GO:0004662 CAAX-protein geranylgeranyltransferase activity [PMID:1400380]" /experiment="EXISTENCE:direct assay:GO:0005953 CAAX-protein geranylgeranyltransferase complex [PMID:1400380]" /experiment="EXISTENCE:direct assay:GO:0005965 protein farnesyltransferase complex [PMID:1763050]" /experiment="EXISTENCE:direct assay:GO:0007323 peptide pheromone maturation [PMID:1763050]" /experiment="EXISTENCE:direct assay:GO:0018343 protein farnesylation [PMID:1763050]" /experiment="EXISTENCE:direct assay:GO:0018344 protein geranylgeranylation [PMID:1400380]" /note="Alpha subunit of farnesyltransferase and geranylgeranyltransferase-I; farnesyltransferase (Ram2p-Ram1p heterodimer) catalyzes the addition of 15-carbon isoprenoid farnesyl to substrate proteins containing a CAAX consensus motif; type I geranylgeranyltransferase (Ram2p-Cdc43p heterodimer) catalyzes the addition of the 20-carbon isoprenoid geranylgeranyl to substrate proteins containing a CaaL consensus motif; required for membrane localization of Ras proteins and a-factor" /db_xref="SGD:S000001502" BEGIN 1 MEEYDYSDVK PLPIETDLQD ELCRIMYTED YKRLMGLARA LISLNELSPR ALQLTAEIID 61 VAPAFYTIWN YRFNIVRHMM SESEDTVLYL NKELDWLDEV TLNNPKNYQI WSYRQSLLKL 121 HPSPSFKREL PILKLMIDDD SKNYHVWSYR KWCCLFFSDF QHELAYASDL IETDIYNNSA 181 WTHRMFYWVN AKDVISKVEL ADELQFIMDK IQLVPQNISP WTYLRGFQEL FHDRLQWDSK 241 VVDFATTFIG DVLSLPIGSP EDLPEIESSY ALEFLAYHWG ADPCTRDNAV KAYSLLAIKY 301 DPIRKNLWHH KINNLN //