LOCUS CCP44682.1 367 aa PRT BCT 27-FEB-2015
DEFINITION Mycobacterium tuberculosis H37Rv Probable isocitrate lyase
AceAa [first part] (isocitrase) (isocitratase) (Icl) protein.
ACCESSION AL123456-1960
PROTEIN_ID CCP44682.1
SOURCE Mycobacterium tuberculosis H37Rv
ORGANISM Mycobacterium tuberculosis H37Rv
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.,
Harris D., Gordon S.V., Eiglmeier K., Gas S., Barry C.E.III.,
Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T.,
Connor R., Davies R., Devlin K., Feltwell T., Gentles S.,
Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J.,
Moule S., Murphy L., Oliver K., Osborne J., Quail M.A.,
Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton J.,
Squares R., Squares S., Sulston J.E., Taylor K., Whitehead S.,
Barrell B.G.
TITLE Deciphering the biology of Mycobacterium tuberculosis from the
complete genome sequence
JOURNAL Nature 393(6685), 537-544(1998).
PUBMED 9634230
REMARK Erratum:[Nature 1998 Nov 12;396(6707):190]
REFERENCE 2
AUTHORS Camus J.C., Pryor M.J., Medigue C., Cole S.T.
TITLE Re-annotation of the genome sequence of Mycobacterium tuberculosis
H37Rv
JOURNAL Microbiology (Reading, Engl.) 148(Pt 10), 2967-2973(2002).
PUBMED 12368430
REFERENCE 3
AUTHORS Lew J.M., Kapopoulou A., Jones L.M., Cole S.T.
TITLE TubercuList--10 years after
JOURNAL Tuberculosis (Edinb) 91(1), 1-7(2011).
PUBMED 20980199
REFERENCE 4 (bases 1 to 4411529)
AUTHORS Parkhill J.
JOURNAL Submitted (11-JUN-1998) to the INSDC. Submitted on behalf of the
Mycobacterium tuberculosis sequencing and mapping teams, Sanger
Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA
Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28
rue du Docteur Roux, 75724 Paris Cedex 15, France E-mail:
parkhill@sanger.ac.uk
REFERENCE 5 (bases 1 to 4411532)
AUTHORS Lew J.M.
JOURNAL Submitted (18-DEC-2012) to the INSDC. Lew J., Ecole Polytechnique
Federale de Lausanne, CH-1015, Lausanne, Switzerland, and the Swiss
Institute of Bioinformatics, CMU - Rue Michel-Servet 1, 1211 Geneva
4, SWITZERLAND
COMMENT On or before Feb 1, 2013 this sequence version replaced
gi:41352722, gi:38490165, gi:38490207, gi:41353619, gi:38490250,
gi:38684030, gi:38490288, gi:41353667, gi:41353422, gi:41352756,
gi:38490319, gi:41352785, gi:38490370, gi:41353971.
Note:
This annotation is from the TubercuList website, Release 26, Dec
2012 (URL: http://tuberculist.epfl.ch) (email:
tuberculist@epfl.ch).
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis H37Rv"
/strain="H37Rv"
/mol_type="genomic DNA"
/db_xref="taxon:83332"
protein /transl_table=11
/gene="aceAa"
/gene_synonym="icl2a"
/locus_tag="Rv1915"
/note="Rv1915, (MTCY180.03c), len: 367 aa. Probable
aceAa,isocitrate lyase (see citations below). Highly
similar to the N-terminus of ACEA_MYCLE isocitrate lyase
from Mycobacterium leprae (606 aa), FASTA results: opt:
3314,E(): 0, (86.5% identity in 572 aa overlap). Contains
PS00161 Isocitrate lyase signature. Although this ORF and
the downstream ORF representing the C-terminal half of
aceA could be joined by a frameshift, no error is apparent
in the cosmid, or in a seqencing read from the genome of
H37Rv. As the downstream ORF has a RBS and transcriptional
start immediately following the stop of this ORF, it is
possible that they are expressed as two separate modules.
In Mycobacterium tuberculosis strain CDC1551, aceA exists
as a single gene, MT1966: the corresponding protein has
been purified experimentally and seems have an active
isocitrate lyase activity (see Honer et al., 1999). For
Mycobacterium tuberculosis strain H37Rv, immunoblot assay
didn't detect AceAa or AceAb products (see Honer et
al.,1999) but mRNA of AceAa|Rv1915 has been detected (see
Betts et al., 2002); so AceAb|Rv1916 could be a
pseudogene. Icl2 has 2-methyl-isocitrate lyase (MCL)
activity in M. tuberculosis Erdman (See Munoz-Elias et
al., 2006)."
/db_xref="EnsemblGenomes-Gn:Rv1915"
/db_xref="EnsemblGenomes-Tr:CCP44682"
/db_xref="GOA:O07718"
/db_xref="InterPro:IPR006254"
/db_xref="InterPro:IPR015813"
/db_xref="InterPro:IPR018523"
/db_xref="InterPro:IPR039556"
/db_xref="InterPro:IPR040442"
/db_xref="UniProtKB/Swiss-Prot:O07718"
/inference="protein motif:PROSITE:PS00161"
/experiment="EXISTENCE: identified in proteomics study"
BEGIN
1 MAIAETDTEV HTPFEQDFEK DVAATQRYFD SSRFAGIIRL YTARQVVEQR GTIPVDHIVA
61 REAAGAFYER LRELFAARKS ITTFGPYSPG QAVSMKRMGI EAIYLGGWAT SAKGSSTEDP
121 GPDLASYPLS QVPDDAAVLV RALLTADRNQ HYLRLQMSER QRAATPAYDF RPFIIADAGT
181 GHGGDPHVRN LIRRFVEVGV PGYHIEDQRP GTKKCGHQGG KVLVPSDEQI KRLNAARFQL
241 DIMRVPGIIV ARTDAEAANL IDSRADERDQ PFLLGATKLD VPSYKSCFLA MVRRFTNWAS
301 RSSMVIFSMR LATASTRRPA VGLSAKAFSA WSPTRSTRGG RTASSRSTAF STRSSRGSWR
361 PGRTTRA
//