LOCUS CCP44682.1 367 aa PRT BCT 27-FEB-2015 DEFINITION Mycobacterium tuberculosis H37Rv Probable isocitrate lyase AceAa [first part] (isocitrase) (isocitratase) (Icl) protein. ACCESSION AL123456-1960 PROTEIN_ID CCP44682.1 SOURCE Mycobacterium tuberculosis H37Rv ORGANISM Mycobacterium tuberculosis H37Rv Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C., Harris D., Gordon S.V., Eiglmeier K., Gas S., Barry C.E.III., Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R., Devlin K., Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L., Oliver K., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton J., Squares R., Squares S., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. TITLE Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence JOURNAL Nature 393(6685), 537-544(1998). PUBMED 9634230 REMARK Erratum:[Nature 1998 Nov 12;396(6707):190] REFERENCE 2 AUTHORS Camus J.C., Pryor M.J., Medigue C., Cole S.T. TITLE Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv JOURNAL Microbiology (Reading, Engl.) 148(Pt 10), 2967-2973(2002). PUBMED 12368430 REFERENCE 3 AUTHORS Lew J.M., Kapopoulou A., Jones L.M., Cole S.T. TITLE TubercuList--10 years after JOURNAL Tuberculosis (Edinb) 91(1), 1-7(2011). PUBMED 20980199 REFERENCE 4 (bases 1 to 4411529) AUTHORS Parkhill J. JOURNAL Submitted (11-JUN-1998) to the INSDC. Submitted on behalf of the Mycobacterium tuberculosis sequencing and mapping teams, Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France E-mail: parkhill@sanger.ac.uk REFERENCE 5 (bases 1 to 4411532) AUTHORS Lew J.M. JOURNAL Submitted (18-DEC-2012) to the INSDC. Lew J., Ecole Polytechnique Federale de Lausanne, CH-1015, Lausanne, Switzerland, and the Swiss Institute of Bioinformatics, CMU - Rue Michel-Servet 1, 1211 Geneva 4, SWITZERLAND COMMENT On or before Feb 1, 2013 this sequence version replaced gi:41352722, gi:38490165, gi:38490207, gi:41353619, gi:38490250, gi:38684030, gi:38490288, gi:41353667, gi:41353422, gi:41352756, gi:38490319, gi:41352785, gi:38490370, gi:41353971. Note: This annotation is from the TubercuList website, Release 26, Dec 2012 (URL: http://tuberculist.epfl.ch) (email: tuberculist@epfl.ch). FEATURES Qualifiers source /organism="Mycobacterium tuberculosis H37Rv" /strain="H37Rv" /mol_type="genomic DNA" /db_xref="taxon:83332" protein /transl_table=11 /gene="aceAa" /gene_synonym="icl2a" /locus_tag="Rv1915" /note="Rv1915, (MTCY180.03c), len: 367 aa. Probable aceAa,isocitrate lyase (see citations below). Highly similar to the N-terminus of ACEA_MYCLE isocitrate lyase from Mycobacterium leprae (606 aa), FASTA results: opt: 3314,E(): 0, (86.5% identity in 572 aa overlap). Contains PS00161 Isocitrate lyase signature. Although this ORF and the downstream ORF representing the C-terminal half of aceA could be joined by a frameshift, no error is apparent in the cosmid, or in a seqencing read from the genome of H37Rv. As the downstream ORF has a RBS and transcriptional start immediately following the stop of this ORF, it is possible that they are expressed as two separate modules. In Mycobacterium tuberculosis strain CDC1551, aceA exists as a single gene, MT1966: the corresponding protein has been purified experimentally and seems have an active isocitrate lyase activity (see Honer et al., 1999). For Mycobacterium tuberculosis strain H37Rv, immunoblot assay didn't detect AceAa or AceAb products (see Honer et al.,1999) but mRNA of AceAa|Rv1915 has been detected (see Betts et al., 2002); so AceAb|Rv1916 could be a pseudogene. Icl2 has 2-methyl-isocitrate lyase (MCL) activity in M. tuberculosis Erdman (See Munoz-Elias et al., 2006)." /db_xref="EnsemblGenomes-Gn:Rv1915" /db_xref="EnsemblGenomes-Tr:CCP44682" /db_xref="GOA:O07718" /db_xref="InterPro:IPR006254" /db_xref="InterPro:IPR015813" /db_xref="InterPro:IPR018523" /db_xref="InterPro:IPR039556" /db_xref="InterPro:IPR040442" /db_xref="UniProtKB/Swiss-Prot:O07718" /inference="protein motif:PROSITE:PS00161" /experiment="EXISTENCE: identified in proteomics study" BEGIN 1 MAIAETDTEV HTPFEQDFEK DVAATQRYFD SSRFAGIIRL YTARQVVEQR GTIPVDHIVA 61 REAAGAFYER LRELFAARKS ITTFGPYSPG QAVSMKRMGI EAIYLGGWAT SAKGSSTEDP 121 GPDLASYPLS QVPDDAAVLV RALLTADRNQ HYLRLQMSER QRAATPAYDF RPFIIADAGT 181 GHGGDPHVRN LIRRFVEVGV PGYHIEDQRP GTKKCGHQGG KVLVPSDEQI KRLNAARFQL 241 DIMRVPGIIV ARTDAEAANL IDSRADERDQ PFLLGATKLD VPSYKSCFLA MVRRFTNWAS 301 RSSMVIFSMR LATASTRRPA VGLSAKAFSA WSPTRSTRGG RTASSRSTAF STRSSRGSWR 361 PGRTTRA //