LOCUS CAD76985.1 1138 aa PRT BCT 27-FEB-2015
DEFINITION Rhodopirellula baltica SH 1 type I restriction enzyme
EcoKI R protein protein.
ACCESSION BX294152-92
PROTEIN_ID CAD76985.1
SOURCE Rhodopirellula baltica SH 1
ORGANISM Rhodopirellula baltica SH 1
Bacteria; Planctomycetes; Planctomycetia; Pirellulales;
Pirellulaceae; Rhodopirellula.
REFERENCE 1 (bases 1 to 304250)
AUTHORS Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T.,
Ludwig W., Gade D., Beck A., Borzym K., Heitmann K., Rabus R.,
Schlesner H., Amann R., Reinhardt R.
TITLE Complete genome sequence of the marine planctomycete Pirellula sp.
strain 1
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 100(14), 8298-8303(2003).
PUBMED 12835416
REFERENCE 2 (bases 1 to 304250)
AUTHORS Kube M., Borzym K., Heitmann K., Klages S., Marquardt I.,
Lehrack S., Beck A., Pawlik R., Reinhardt R., Gloeckner F.O.,
Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Rabus R.,
Schlesner H., Amann R.
JOURNAL Submitted (21-JAN-2003) to the INSDC. Max Planck Institute for
Molecular Genetics, proScience Ihnestrasse 73, D-14195 Berlin,
Germany Max Planck Institute for Marine Microbiology Celsiusstrasse
1, D-28359 Bremen, Germany.
COMMENT This project was carried out by
*Max Planck Institute for Molecular Genetics, Berlin, Germany;
*Max Planck Institute for Marine Microbiology, Bremen, Germany;
in the fremework of the REGX-project, http://www.regx.de
-------------- Genome Center
Center: Max Planck Institute for Molecular Genetics
Center code: MPIMG
-------------- Summary Statistics
Sequencing vector: pUC19; 100% of reads
Chemistry: Dye-terminator Big Dye; 100% of reads
Assembly program: Phrap; version 0.990329
Consensus quality: 7142841 bases at least Q40
Consensus quality: 7145138 bases at least Q30
Consensus quality: 7145484 bases at least Q20
Quality coverage: 8.03
--------------
This sequence was finished as follows unless otherwise noted:
all regions were double stranded, sequenced with an alternate
chemistry, or coveredby high quality data (i.e., phred quality
>= 30); an attempt was made to resolve all sequencing problems,
such as compressions and repeats; allregions were covered by at
least one plasmid Sequence; assembly was additionally confirmed
by long range pcr and cosmid end sequences.
--------------
See http://www.micro-genomes.mpg.de/pirellula/ for more information
including minimal tiling path from a set of 220 cosmids out of
908.
See the misc_feature tag below for the boundaries of the MTP
cosmids.
-------------- Annotation
Center: Max Planck Institute for Marine Microbiology
Celsiusstrasse 1, D-28359 Bremen, Germany.
Center Code: MPIMM
Email: fog@mpi-bremen.de
Phone: +49 (0)421 2028 938 Fax: +49 (0)421 2028 580
--------------
Three different programs (Glimmer, Critica and Orpheus) were used
for ORF-prediction. A nonredundant list of ORFs was generated by
suitable parsing of the results.
Automated annotation was done with the software package Pedant Pro
(http://www.biomax.de). All ORF predictions and annotations were
manually corrected by considering all results of the different
tools applied. See http://www.regx.de for more information and
access to supplementary information.
--------------
FEATURES Qualifiers
source /organism="Rhodopirellula baltica SH 1"
/strain="1"
/mol_type="genomic DNA"
/db_xref="taxon:243090"
protein /transl_table=11
/gene="hsdR"
/locus_tag="RB10770"
/function="DNA-restriction and modification"
/EC_number="3.1.21.3"
/note="PMID: 3323532 PMID: 1650347 best DB hits: BLAST:
swissprot:P08956; T1RK_ECOLI TYPE I RESTRICTION ENZYME
ECOKI R; E=0.0 embl:CAA29791.1; (X06545) hsdR gene product
(AA 1 - 1090); E=1e-158 gb:AAF43698.1; AF232829_1
(AF232829) unknown [Mycobacterium avium]; E=8e-38 COG:
hsdR; COG0610 Restriction enzymes type I helicase subunits
and related; E=0.0 YDR332w; COG1061 DNA or RNA helicases
of superfamily II; E=7e-14 jhp1424; COG0610 Restriction
enzymes type I helicase subunits and; E=3e-04 PFAM:
PF00270; DEAD/DEAH box helicase; E=0.047 PF00271; Helicase
conserved C-terminal doma; E=1.3e-10"
/db_xref="GOA:Q7UK96"
/db_xref="InterPro:IPR001650"
/db_xref="InterPro:IPR006935"
/db_xref="InterPro:IPR007409"
/db_xref="InterPro:IPR013670"
/db_xref="InterPro:IPR014001"
/db_xref="InterPro:IPR025285"
/db_xref="InterPro:IPR027417"
/db_xref="UniProtKB/TrEMBL:Q7UK96"
BEGIN
1 MGQGETPKDH PGNFWFLEGY QPVLLAHALR AERYVFDDPN TALVKLRQLA EFFAREVAAE
61 LGIATGSQED FVNVERRLRD SGILNQQLRD VMRTVRQKGN SAAHSLAGER RDALHCLKLV
121 RQLAIWFHKT VRGANNFHAG PFVPPADPAQ GDEQLSEELA RLRETVEKQQ AELDAAKAES
181 EKLGEVVRDR QSAYELAKGN EQAAMELAAE TEETLQQRIE DYERLLSETA AKNAEQSAEA
241 REDIIEASQK AAQEISLDEF DTRKLIDQQL RDAGWEADTS ELRYANGIRP QKGRNMAIAE
301 VPTDDGIADY VLFTGLTPIA TVEAKRKRRN ARSAIDQAHR YSKSFRMSSD YVQPGGPWGE
361 YSVPFVFSTN GTPFHRQLID QSGIWFRDVR VETNHPRALT GWYTPEGLQD LLKLDIPAAN
421 AKLETEPRDY LPLRYYQRDA IEAVEKAIAK DQTEILLAMA TGTGKTRTAI CLLYRLIKAS
481 RFNRILFVVD RRSLGEQAFD AFKDVKLEQN QSFPEIYDVK ELGDVKPDRE TKLHLCTIQS
541 MVKRVVDQDS NSEPYPVDQY DCIVIDECHR GYTLDRDMSE EELGYRDQLD YVSKYRRVLD
601 HFDAVRIGLT ATPALHTTEI FGSPVYQYSY RQAVIDNNLV DHEPPFIILT ELSESGMHWD
661 AGETVKYFDT VTKSIADETT PDEIDIEIDG FNRLAITESF NQVVCDVLAQ QIDHTQPGKT
721 LIFCVRDTHA DMVVRLLTAA IEQRHGPQPH DTVKKITGDI ERTSEAIRRF KNEPIPKYVV
781 TVDLLTTGID VPEITNVVFL RRTKSRILFD QMIGRATRLC KDLFFDGEDK EYFHIYDAVG
841 VYEALQDHTD MKPVVAKPFT MFKKLVELFQ SSSDPDAHAD IRQQLVAKLR RKTPSINKNW
901 LDTFELLAGD TPEAISDLWA SSSDAELQQW LTDHASLITE LDQRKADGSI IISEHADALV
961 GIERGYGGNQ KPEDYLESFR QFLVEHKTDI PALILVTTRP RDLTRKDLKE LQLQLEQHGF
1021 NETAIKSAVK DTTNQDIAAS ILGFVRHAML NEPLLPYEER VDEAMRSILT SQKWQPQQRK
1081 WLERIGKQIK ENVVIDRDLF EVGAFQDHGG FVKIDKVFDG RLNEVMQSII TSIWQTAT
//