LOCUS CAD76985.1 1138 aa PRT BCT 27-FEB-2015 DEFINITION Rhodopirellula baltica SH 1 type I restriction enzyme EcoKI R protein protein. ACCESSION BX294152-92 PROTEIN_ID CAD76985.1 SOURCE Rhodopirellula baltica SH 1 ORGANISM Rhodopirellula baltica SH 1 Bacteria; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Rhodopirellula. REFERENCE 1 (bases 1 to 304250) AUTHORS Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Beck A., Borzym K., Heitmann K., Rabus R., Schlesner H., Amann R., Reinhardt R. TITLE Complete genome sequence of the marine planctomycete Pirellula sp. strain 1 JOURNAL Proc. Natl. Acad. Sci. U.S.A. 100(14), 8298-8303(2003). PUBMED 12835416 REFERENCE 2 (bases 1 to 304250) AUTHORS Kube M., Borzym K., Heitmann K., Klages S., Marquardt I., Lehrack S., Beck A., Pawlik R., Reinhardt R., Gloeckner F.O., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Rabus R., Schlesner H., Amann R. JOURNAL Submitted (21-JAN-2003) to the INSDC. Max Planck Institute for Molecular Genetics, proScience Ihnestrasse 73, D-14195 Berlin, Germany Max Planck Institute for Marine Microbiology Celsiusstrasse 1, D-28359 Bremen, Germany. COMMENT This project was carried out by *Max Planck Institute for Molecular Genetics, Berlin, Germany; *Max Planck Institute for Marine Microbiology, Bremen, Germany; in the fremework of the REGX-project, http://www.regx.de -------------- Genome Center Center: Max Planck Institute for Molecular Genetics Center code: MPIMG -------------- Summary Statistics Sequencing vector: pUC19; 100% of reads Chemistry: Dye-terminator Big Dye; 100% of reads Assembly program: Phrap; version 0.990329 Consensus quality: 7142841 bases at least Q40 Consensus quality: 7145138 bases at least Q30 Consensus quality: 7145484 bases at least Q20 Quality coverage: 8.03 -------------- This sequence was finished as follows unless otherwise noted: all regions were double stranded, sequenced with an alternate chemistry, or coveredby high quality data (i.e., phred quality >= 30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; allregions were covered by at least one plasmid Sequence; assembly was additionally confirmed by long range pcr and cosmid end sequences. -------------- See http://www.micro-genomes.mpg.de/pirellula/ for more information including minimal tiling path from a set of 220 cosmids out of 908. See the misc_feature tag below for the boundaries of the MTP cosmids. -------------- Annotation Center: Max Planck Institute for Marine Microbiology Celsiusstrasse 1, D-28359 Bremen, Germany. Center Code: MPIMM Email: fog@mpi-bremen.de Phone: +49 (0)421 2028 938 Fax: +49 (0)421 2028 580 -------------- Three different programs (Glimmer, Critica and Orpheus) were used for ORF-prediction. A nonredundant list of ORFs was generated by suitable parsing of the results. Automated annotation was done with the software package Pedant Pro (http://www.biomax.de). All ORF predictions and annotations were manually corrected by considering all results of the different tools applied. See http://www.regx.de for more information and access to supplementary information. -------------- FEATURES Qualifiers source /organism="Rhodopirellula baltica SH 1" /strain="1" /mol_type="genomic DNA" /db_xref="taxon:243090" protein /transl_table=11 /gene="hsdR" /locus_tag="RB10770" /function="DNA-restriction and modification" /EC_number="3.1.21.3" /note="PMID: 3323532 PMID: 1650347 best DB hits: BLAST: swissprot:P08956; T1RK_ECOLI TYPE I RESTRICTION ENZYME ECOKI R; E=0.0 embl:CAA29791.1; (X06545) hsdR gene product (AA 1 - 1090); E=1e-158 gb:AAF43698.1; AF232829_1 (AF232829) unknown [Mycobacterium avium]; E=8e-38 COG: hsdR; COG0610 Restriction enzymes type I helicase subunits and related; E=0.0 YDR332w; COG1061 DNA or RNA helicases of superfamily II; E=7e-14 jhp1424; COG0610 Restriction enzymes type I helicase subunits and; E=3e-04 PFAM: PF00270; DEAD/DEAH box helicase; E=0.047 PF00271; Helicase conserved C-terminal doma; E=1.3e-10" /db_xref="GOA:Q7UK96" /db_xref="InterPro:IPR001650" /db_xref="InterPro:IPR006935" /db_xref="InterPro:IPR007409" /db_xref="InterPro:IPR013670" /db_xref="InterPro:IPR014001" /db_xref="InterPro:IPR025285" /db_xref="InterPro:IPR027417" /db_xref="UniProtKB/TrEMBL:Q7UK96" BEGIN 1 MGQGETPKDH PGNFWFLEGY QPVLLAHALR AERYVFDDPN TALVKLRQLA EFFAREVAAE 61 LGIATGSQED FVNVERRLRD SGILNQQLRD VMRTVRQKGN SAAHSLAGER RDALHCLKLV 121 RQLAIWFHKT VRGANNFHAG PFVPPADPAQ GDEQLSEELA RLRETVEKQQ AELDAAKAES 181 EKLGEVVRDR QSAYELAKGN EQAAMELAAE TEETLQQRIE DYERLLSETA AKNAEQSAEA 241 REDIIEASQK AAQEISLDEF DTRKLIDQQL RDAGWEADTS ELRYANGIRP QKGRNMAIAE 301 VPTDDGIADY VLFTGLTPIA TVEAKRKRRN ARSAIDQAHR YSKSFRMSSD YVQPGGPWGE 361 YSVPFVFSTN GTPFHRQLID QSGIWFRDVR VETNHPRALT GWYTPEGLQD LLKLDIPAAN 421 AKLETEPRDY LPLRYYQRDA IEAVEKAIAK DQTEILLAMA TGTGKTRTAI CLLYRLIKAS 481 RFNRILFVVD RRSLGEQAFD AFKDVKLEQN QSFPEIYDVK ELGDVKPDRE TKLHLCTIQS 541 MVKRVVDQDS NSEPYPVDQY DCIVIDECHR GYTLDRDMSE EELGYRDQLD YVSKYRRVLD 601 HFDAVRIGLT ATPALHTTEI FGSPVYQYSY RQAVIDNNLV DHEPPFIILT ELSESGMHWD 661 AGETVKYFDT VTKSIADETT PDEIDIEIDG FNRLAITESF NQVVCDVLAQ QIDHTQPGKT 721 LIFCVRDTHA DMVVRLLTAA IEQRHGPQPH DTVKKITGDI ERTSEAIRRF KNEPIPKYVV 781 TVDLLTTGID VPEITNVVFL RRTKSRILFD QMIGRATRLC KDLFFDGEDK EYFHIYDAVG 841 VYEALQDHTD MKPVVAKPFT MFKKLVELFQ SSSDPDAHAD IRQQLVAKLR RKTPSINKNW 901 LDTFELLAGD TPEAISDLWA SSSDAELQQW LTDHASLITE LDQRKADGSI IISEHADALV 961 GIERGYGGNQ KPEDYLESFR QFLVEHKTDI PALILVTTRP RDLTRKDLKE LQLQLEQHGF 1021 NETAIKSAVK DTTNQDIAAS ILGFVRHAML NEPLLPYEER VDEAMRSILT SQKWQPQQRK 1081 WLERIGKQIK ENVVIDRDLF EVGAFQDHGG FVKIDKVFDG RLNEVMQSII TSIWQTAT //