LOCUS CAB36627.1 1540 aa PRT BCT 23-OCT-2008
DEFINITION Mycobacterium leprae putative polyketide synthase protein.
ACCESSION AL035480-1
PROTEIN_ID CAB36627.1
SOURCE Mycobacterium leprae
ORGANISM Mycobacterium leprae
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium.
REFERENCE 1 (bases 1 to 34316)
AUTHORS Murphy L., Harris D.
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 34316)
AUTHORS James K.D., Parkhill J., Barrell B.G., Rajandream M.A.
JOURNAL Submitted (15-FEB-1999) to the INSDC. Mycobacterium leprae
sequencing project, Sanger Centre, Wellcome Trust Genome Campus,
Hinxton, Cambridge CB10 1SA E-mail: barrell@sanger.ac.uk Cosmids
supplied by Dr. Stewart T. Cole, [3] Unite de Genetique Moleculaire
Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris
Cedex 15, France Requests for cosmids should be sent to Karin
Eiglmeier (kei@pasteur.fr).
REFERENCE 3 (bases 1 to 34316)
AUTHORS Eiglmeier K., Honore N., Woods S.A., Caudron B., Cole S.T.
TITLE Use of an ordered cosmid library to deduce the genomic organization
of Mycobacterium leprae
JOURNAL Mol. Microbiol. 7(2), 197-206(1993).
PUBMED 8446027
COMMENT Notes:
The Sanger Centre is funded to complete the sequence of M. leprae
by the Heiser Program for Research in Leprosy and Tuberculosis of
The New York Community Trust.
Work in Paris is supported by the Heiser Trust, the Association
Francaise Raoul Follereau and the Groupement de Recherches et des
Etudes des Genomes (GIP-GREG).
Details of M. leprae sequencing at the Sanger Centre
are available on the World Wide Web.
(URL, http://www.sanger.ac.uk/Projects/)
CDS are numbered using the following system eg MLCB33.01c.
ML (M. leprae), cB33 (cosmid name), .01 (first CDS),
c (complementary strand).
The more significant matches with motifs in the PROSITE
database are also included but some of these may be fortuitous.
The length in codons is given for each CDS.
Usually the highest scoring match found by fasta -o is given for
CDS which show significant similarity to other CDS in the database.
The position of possible ribosome binding site sequences are
given where these have been used to deduce the initiation codon.
All CDS over 100 codons have been analysed. Gene prediction
is based on positional base preference in codons especially
where there is an increase in the observed/expected third
position G + C. CAUTION: We may not have predicted the
correct initiation codon. Where possible we choose an
initiation codon (atg, gtg, or ttg) which is preceded by an
upstream ribosome binding site sequence (optimally 5-13bp
before the initiation codon). If this cannot be identified
we choose the most upstream initiation codon.
IMPORTANT: This sequence MAY NOT be the entire insert of
the sequenced clone. It may be shorter because we only
sequence overlapping sections once, or longer, because we
arrange for a small overlap between neighbouring submissions.
FEATURES Qualifiers
source /organism="Mycobacterium leprae"
/mol_type="genomic DNA"
/clone="cosmid B12"
/db_xref="taxon:1769"
protein /transl_table=11
/gene="MLCB12.01c"
/note="MLCB12.01c, probable polyketide synthase, len: 1540
aa; similar to ERY1_SACER (EMBL:M63676) Saccharopolyspora
erythraea erythronolide synthase (3491 aa), fasta scores;
opt: 2230 z-score: 2283.7 E(): 0, 36.6% identity in 1550
aa overlap. N-terminus similar to the N-terminus of
MCAS_MYCBO (EMBL:M95808) Mycobacterium bovis mycocerosic
acid synthase (2110 aa) (42.2% identity in 879 aa
overlap). Also similar to many mycobacterial putative
polyketide synthases e.g. ppsB, Rv2932,
(MTV011.01-MTCY338.21) M.tuberculosis putative polyketide
synthase involved in phenolpthiocerol synthesis (1538 aa)
(76.3% identity in 1561 aa overlap). Annotated as ORF
TR:Q49932, designated pksC in M.leprae cosmid EMBL:U00023.
Contains Pfam match to entry PF00550 pp-binding,
Phosphopantetheine attachment site, score 42.90, E-value
3e-10, Pfam match to entry PF00698 Acyl_transf,
Acyltransferase domain, score 467.00, E-value 1.6e-136,
Pfam match to entry PF001 09 ketoacyl-synt, Beta-ketoacyl
synthase, score 694.00, E-value 7.4e-205, Pfam match to
entry PF00501 AMP-binding, AMP-binding enzyme, score
-162.30, E-value 4.9e-06. Contains PS00606 Beta-ketoacyl
synthases active site"
/db_xref="GOA:Q9S384"
/db_xref="InterPro:IPR009081"
/db_xref="InterPro:IPR013968"
/db_xref="InterPro:IPR014030"
/db_xref="InterPro:IPR014031"
/db_xref="InterPro:IPR014043"
/db_xref="InterPro:IPR016035"
/db_xref="InterPro:IPR016036"
/db_xref="InterPro:IPR016039"
/db_xref="InterPro:IPR018201"
/db_xref="InterPro:IPR020801"
/db_xref="InterPro:IPR020806"
/db_xref="InterPro:IPR020841"
/db_xref="InterPro:IPR032821"
/db_xref="InterPro:IPR036291"
/db_xref="InterPro:IPR036736"
/db_xref="UniProtKB/TrEMBL:Q9S384"
BEGIN
1 MRTAFSRISG MTTQQRAALI EEFTKLSRIA VAEPIAVVGI GCRFPGDVTG PDSFWDLLID
61 GRNAISRVPA DRWDADAFYD PDPLTPGRMT TKWGGFVSDI AGFDAAFFGI TPREAAAMDP
121 QQRILLEVAW EALENAGIPP DSLGNSRTGV MIGVYFNEYQ SMLASSLENV DAYSGTGNAH
181 SITVGRISYL LGLRGPSVAV DTACSSSLVA VHLACQSLRL RETDLVLAGG VSITLRPETQ
241 IAISAWGLLS PHGRCAAFDA AADGFVRGEG AGVVVLKRLT DAVRDGDLVL AVVRGSAVNQ
301 DGRSNGVTAP NTAAQCDVIT DALRSSDVAP ESVNYVESHG TGTVLGDPIE FEALAATYGR
361 GESACALGAV KTNLGHLEAA AGIAGFIKTV LAVQRGQIPP NLHFSQWNPA IDAASTRFFV
421 PTENSSWPIS DGQAGPRRAA VSSFGLGGTN AHVVIEQGPE LTPVTECSSN TAVSTLVVTG
481 KTASRVAAMA GMLADWVEGP GAEVALADVA HTLNHHRSRH AKFGTVVARD RIQAVAGLRA
541 LAAGKQAPGV VGQQDGTPGS GTVFVYSGRG SQWAGMGRQL LADEPAFTAA VAELEPVFVV
601 HAGFSLHDVL ANGKELVGIE QIQLGLIGMQ LTLTELWRSY GVQPDLVIGH SLGEVAAAVV
661 AGALTAAEGL RVTATRSRLM APLSGQGGMA LLELDAVETE ALIVDYSQVT LAIYNSPRQT
721 VIAGPTEQID ELIDRVRAQN RFASRVNIEV APHNPAMDAL QPQMRSELAD VAPRTPTIPI
781 LSTTYADLGS CPVFDAQHWA TNMRNPVHFQ QAIMTAGTDH RTFIEISAHP LLTQAITDTL
841 HGTRCISIGT LQRDADDTVT FHTNLNNVHT VHPPHTPHPA EPHVTIPSTP WQHTRHWITT
901 PLASTTALQH LDRNRVTTHA TGIGNTELDD WIYQLDWPTR PLTSEPGATW ASSGSWLVVS
961 DAGLSDELAR LVVLADPASR VEYLAPSALD HDVSTLHDLH NALRGVDNVL YAPSVSTESV
1021 DPVDPESGYR LFHAVRRLAA AMVAGTSKPK LVVVTRNAQP VAVGERANPA HAVLWGFGRT
1081 LALEHPEIWG SVIDLDASMP PELAARCILD EVASQDEKDR EDQVVYRACL RRAPRLQRRT
1141 ASAVSSVKLS SGQDTTQLVI GATGNIGPHL IRQLAAMGAT TIVAVSRNPS TRLLELDKNL
1201 AATGTNLISV AADATDPTAM TALFDRFGVD LPPLEGIYLA AFAGRPVLLS DMTDNDVVAM
1261 FRPKLDVASL LHRLSLKQPV KYFLLFSSIS GLIGSRWLAH YTATSAFLDI LGYARRLVGL
1321 PATVVAWGLW KSLADAQQDA TQVSAESGLQ PMTDDVAIGA LPLVMSPDAP VHSVVAAADW
1381 PLLAAAYQTR GVLRMVDDLL PAPGEVLMQE SEFRKALRTC QAERRHNMLF DHVSALVAKA
1441 MGLLPAETLD PSAGFFQLGM DSLMSVTLQR ALSDSLGEFL PASVVFDYPT VYSLTDYLAT
1501 ILPEFLEPEA PSGRTTADAY DEFTESELLD QLSERLRGTQ
//