LOCUS AKO88593.1 448 aa PRT BCT 27-AUG-2015
DEFINITION Pseudomonas aeruginosa DSM 50071 = NBRC 12689 UDP-N-acetylmuramoyl-
L-alanyl-D-glutamate synthetase protein.
ACCESSION CP012001-4528
PROTEIN_ID AKO88593.1
SOURCE Pseudomonas aeruginosa DSM 50071 = NBRC 12689
ORGANISM Pseudomonas aeruginosa DSM 50071 = NBRC 12689
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
REFERENCE 1 (bases 1 to 6317050)
AUTHORS Nakano,K., Terabayashi,Y., Shiroma,A., Shimoji,M., Tamotsu,H.,
Ashimine,N., Ohki,S., Shinzato,M., Teruya,K., Satou,K. and
Hirano,T.
TITLE First Complete Genome Sequence of Pseudomonas aeruginosa (Schroeter
1872) Migula 1900 (DSM 50071T), Determined Using PacBio
Single-Molecule Real-Time Technology
JOURNAL Genome Announc 3 (4), e00932-15 (2015)
PUBMED 26294631
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 6317050)
AUTHORS Nakano,K., Terabayashi,Y., Shiroma,A., Shimoji,M., Tamotsu,H.,
Ashimine,N., Ohki,S., Shinzato,M., Teruya,K., Satou,K. and
Hirano,T.
TITLE Direct Submission
JOURNAL Submitted (01-JUL-2015) Research and Development Division, Okinawa
Institute of Advanced Sciences, Okinawa Biotechnology Business
Support Center, Suite109, 5-1, Suzaki, Uruma, Okinawa 904-2234,
Japan
COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (released 2013). Information about the Pipeline can be
found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
##Genome-Assembly-Data-START##
Assembly Method :: HGAP3 v. 2.3.0
Genome Coverage :: 843x
Sequencing Technology :: PacBio
##Genome-Assembly-Data-END##
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Date :: 07/02/2015 10:33:57
Annotation Pipeline :: NCBI Prokaryotic Genome Annotation
Pipeline
Annotation Method :: Best-placed reference protein set;
GeneMarkS+
Annotation Software revision :: 2.10
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA;
repeat_region
Genes :: 5,805
CDS :: 5,710
Pseudo Genes :: 18
CRISPR Arrays :: 3
rRNAs :: 12 (5S, 16S, 23S)
tRNAs :: 64
ncRNA :: 1
Frameshifted Genes :: 11
##Genome-Annotation-Data-END##
FEATURES Qualifiers
source /organism="Pseudomonas aeruginosa DSM 50071 = NBRC 12689"
/mol_type="genomic DNA"
/strain="DSM 50071"
/culture_collection="DSM:50071"
/type_material="type strain of Pseudomonas aeruginosa"
/db_xref="taxon:1123015"
protein /gene="murD"
/locus_tag="PA50071_22975"
/EC_number="6.3.2.9"
/inference="EXISTENCE: similar to AA
sequence:RefSeq:WP_017518102.1"
/note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
involved in peptidoglycan biosynthesis; cytoplasmic;
catalyzes the addition of glutamate to the nucleotide
precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
formation; Derived by automated computational analysis
using gene prediction method: Protein Homology."
/transl_table=11
BEGIN
1 MSLIASDHFR IVVGLGKSGM SLVRYLARRG LPFAVVDTRE NPPELATLRA QYPQVEVRCG
61 ELDAEFLCSA RELYVSPGLS LRTPALVQAA AKGVRISGDI DLFAREAKAP IVAITGSNAK
121 STVTTLVGEM AVAADKRVAV GGNLGTPALD LLADDIELYV LELSSFQLET CDRLNAEVAT
181 VLNVSEDHMD RYDGMADYHL AKHRIFRGAR QVVVNRADAL TRPLIADTVP CWSFGLNKPD
241 FKAFGLIEED GQKWLAFQFD KLLPVGELKI RGAHNYSNAL AALALGHAVG LPFDAMLGAL
301 KAFSGLAHRC QWVRERQGVS YYDDSKATNV GAALAAIEGL GADIDGKLVL LAGGDGKGAD
361 FHDLREPVAR FCRAVVLLGR DAGLIAQALG NAVPLVRVAT LDEAVRQAAE LAREGDAVLL
421 SPACASLDMF KNFEERGRLF AKAVEELA
//