LOCUS       AKO88593.1               448 aa    PRT              BCT 27-AUG-2015
DEFINITION  Pseudomonas aeruginosa DSM 50071 = NBRC 12689 UDP-N-acetylmuramoyl-
            L-alanyl-D-glutamate synthetase protein.
ACCESSION   CP012001-4528
PROTEIN_ID  AKO88593.1
SOURCE      Pseudomonas aeruginosa DSM 50071 = NBRC 12689
  ORGANISM  Pseudomonas aeruginosa DSM 50071 = NBRC 12689
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6317050)
  AUTHORS   Nakano,K., Terabayashi,Y., Shiroma,A., Shimoji,M., Tamotsu,H.,
            Ashimine,N., Ohki,S., Shinzato,M., Teruya,K., Satou,K. and
            Hirano,T.
  TITLE     First Complete Genome Sequence of Pseudomonas aeruginosa (Schroeter
            1872) Migula 1900 (DSM 50071T), Determined Using PacBio
            Single-Molecule Real-Time Technology
  JOURNAL   Genome Announc 3 (4), e00932-15 (2015)
   PUBMED   26294631
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317050)
  AUTHORS   Nakano,K., Terabayashi,Y., Shiroma,A., Shimoji,M., Tamotsu,H.,
            Ashimine,N., Ohki,S., Shinzato,M., Teruya,K., Satou,K. and
            Hirano,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2015) Research and Development Division, Okinawa
            Institute of Advanced Sciences, Okinawa Biotechnology Business
            Support Center, Suite109, 5-1, Suzaki, Uruma, Okinawa 904-2234,
            Japan
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: HGAP3 v. 2.3.0
            Genome Coverage       :: 843x
            Sequencing Technology :: PacBio
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 07/02/2015 10:33:57
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS+
            Annotation Software revision :: 2.10
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA;
                                            repeat_region
            Genes                        :: 5,805
            CDS                          :: 5,710
            Pseudo Genes                 :: 18
            CRISPR Arrays                :: 3
            rRNAs                        :: 12 (5S, 16S, 23S)
            tRNAs                        :: 64
            ncRNA                        :: 1
            Frameshifted Genes           :: 11
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Pseudomonas aeruginosa DSM 50071 = NBRC 12689"
                     /mol_type="genomic DNA"
                     /strain="DSM 50071"
                     /culture_collection="DSM:50071"
                     /type_material="type strain of Pseudomonas aeruginosa"
                     /db_xref="taxon:1123015"
     protein         /gene="murD"
                     /locus_tag="PA50071_22975"
                     /EC_number="6.3.2.9"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_017518102.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /transl_table=11
BEGIN
        1 MSLIASDHFR IVVGLGKSGM SLVRYLARRG LPFAVVDTRE NPPELATLRA QYPQVEVRCG
       61 ELDAEFLCSA RELYVSPGLS LRTPALVQAA AKGVRISGDI DLFAREAKAP IVAITGSNAK
      121 STVTTLVGEM AVAADKRVAV GGNLGTPALD LLADDIELYV LELSSFQLET CDRLNAEVAT
      181 VLNVSEDHMD RYDGMADYHL AKHRIFRGAR QVVVNRADAL TRPLIADTVP CWSFGLNKPD
      241 FKAFGLIEED GQKWLAFQFD KLLPVGELKI RGAHNYSNAL AALALGHAVG LPFDAMLGAL
      301 KAFSGLAHRC QWVRERQGVS YYDDSKATNV GAALAAIEGL GADIDGKLVL LAGGDGKGAD
      361 FHDLREPVAR FCRAVVLLGR DAGLIAQALG NAVPLVRVAT LDEAVRQAAE LAREGDAVLL
      421 SPACASLDMF KNFEERGRLF AKAVEELA
//