LOCUS ADU49215.1 1934 aa PRT BCT 08-JAN-2015
DEFINITION Intrasporangium calvum DSM 43043 alpha-1,6-glucosidase,
pullulanase-type protein.
ACCESSION CP002343-2653
PROTEIN_ID ADU49215.1
SOURCE Intrasporangium calvum DSM 43043
ORGANISM Intrasporangium calvum DSM 43043
Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae;
Intrasporangium.
REFERENCE 1 (bases 1 to 4024382)
AUTHORS Del Rio,T.G., Chertkov,O., Yasawong,M., Lucas,S., Deshpande,S.,
Cheng,J.F., Detter,C., Tapia,R., Han,C., Goodwin,L., Pitluck,S.,
Liolios,K., Ivanova,N., Mavromatis,K., Pati,A., Chen,A.,
Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D.,
Rohde,M., Pukall,R., Sikorski,J., Goker,M., Woyke,T., Bristow,J.,
Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P.
and Lapidus,A.
TITLE Complete genome sequence of Intrasporangium calvum type strain (7
KIP)
JOURNAL Stand Genomic Sci 3 (3), 294-303 (2010)
PUBMED 21304734
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 4024382)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
Chertkov,O., Brettin,T., Tapia,R., Detter,J.C., Han,C., Land,M.,
Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Intrasporangium calvum DSM 43043
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 4024382)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
Chertkov,O., Brettin,T., Detter,J.C., Tapia,R., Han,C., Land,M.,
Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (17-NOV-2010) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4083316
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##MIGS-Data-START##
investigation_type :: bacteria_archaea
project_name :: Intrasporangium calvum 7KIP, DSM 43043
collection_date :: 1967
lat_lon :: 55.753 37.604
depth :: Missing
alt_elev :: Missing
country :: Russia
environment :: Air
num_replicons :: Missing
ref_biomaterial :: DSM 43043, ATCC 23552, CUB 430, IFO 12989,
IMET 7816, JCM 3097, KCC A-0097, NBRC 12989,
NCIB 10167
biotic_relationship :: Free living
trophic_level :: Chemoorganotroph
rel_to_oxygen :: Aerobe
isol_growth_condt :: Missing
sequencing_meth :: WGS
assembly :: Newbler v. 2.3 (pre-release)
finishing_strategy :: Finished
GOLD Stamp ID :: Gi02320
Type Strain :: Yes
Greengenes ID :: 90541
Funding Program :: DOE-GEBA 2007
Number of Reads :: 847906 pyrosequencing, 11758818 Illumina
Gene Calling Method :: Prodigal 1.4, GenePRIMP
Isolation Site :: Air in a school dining room
Isolation Comments :: isolated by L.V. Kalakoutskii
Motility :: Nonmotile
Sporulation :: Nonsporulating
Temperature Range :: Mesophile
Temperature Optimum :: 37C
Gram Staining :: Gram+
Diseases :: None
##MIGS-Data-END##
##Genome-Assembly-Data-START##
Finishing Goal :: Finished
Current Finishing Status :: Finished
Assembly Method :: Newbler v. 2.3
Genome Coverage :: 30x
Sequencing Technology :: 454-FLX, 454-Titanium, Illumina GAii
##Genome-Assembly-Data-END##
FEATURES Qualifiers
source /organism="Intrasporangium calvum DSM 43043"
/mol_type="genomic DNA"
/strain="DSM 43043"
/isolation_source="air in a school dining room"
/culture_collection="DSM:43043"
/type_material="type strain of Intrasporangium calvum"
/db_xref="taxon:710696"
/country="Russia"
/lat_lon="55.753 N 37.604 E"
/collection_date="1967"
/collected_by="L.V. Kalakoutskii"
protein /locus_tag="Intca_2714"
/note="COGs: COG1523 Type II secretory pathway pullulanase
PulA and related glycosidase; InterPro IPR006589:
IPR011839: IPR006047: IPR004193; KEGG: kfl:Kfla_2775
alpha-1,6-glucosidase, pullulanase-type; PFAM: alpha
amylase catalytic region; glycoside hydrolase family 13
domain protein; SMART: alpha amylase catalytic sub domain;
SPTR: Alpha-1,6-glucosidase, pullulanase-type; TIGRFAM:
alpha-1,6-glucosidase, pullulanase-type; PFAM: Domain of
unknown function (DUF3372); Alpha amylase, catalytic
domain; Carbohydrate-binding module 48 (Isoamylase
N-terminal domain); TIGRFAM: alpha-1,6-glucosidases,
pullulanase-type"
/transl_table=11
BEGIN
1 MRRRPFLSAA TVAAVAAAGL VTPVSGAVTA WAGDRTVTLV GSLQDELGCP GDWDPGCSAT
61 ELVLEAGSAA YAKVFDVPKG SYELKVAINR SWDENYGADG AKGGANLPLV LEGPARIRFS
121 YDDVSHQIGI APVELSGPAT DADAALATPS LRQAPTKERF YFVMADRFAN GDPSNDDGGL
181 AGDRLATGYD PTDKGFYHGG DLAGISDRLD YIKGLGTTAI WLTPSFKNRP VQGSGDDVSA
241 GYHGYWITDF TQIDPHLGTN AEMTALIDAA HAKGMKVYFD IITNHTADVI DYEGDSDDYR
301 SKEAHPYQDA NGTVFDDTDV AGTSDFPTLD PATSFPYVPT FRTPQDETVK VPAWLNDPVR
361 YHNRGNSTFA GESSLYGDFF GLDDLFTEDP VVVKGMEDIY EAWVDLGIDG FRIDTVKHVN
421 LPFWRSFGPA LMEHAKAAGN DDFFMFGEVF DGSPAYMSRY TTEGRLPATL DFGFQGAALG
481 FASGTPTTGL RDLFAGDDYY TDLDSNAYSL PTFLGNHDMG RVGAMLRDAE FGGDELLART
541 ELANSLMFLT RGQPVVYYGD EQGFTGAGGD KDARQDMFAT QVADYEDDVI IDGGADEPMG
601 SADRYGTAGP LYTHTARLAA LRQAHPALAD GAQLHRYASD SAGIYAVSRI DKNARREYVV
661 VANNATEAKT ATFATASPKT SFLPLLGTSK GVTSDRAGRV TVTVPPLSVQ VFRAKTAFHA
721 PESAITVHPA APAAGGVVGG RAEIGAAMPG NAFAEVTFLQ RPVGTTAWTP IGTDDNAPYR
781 VFHDVTGLAK GTLLEYRMVA RDIEGNLAAT STYGIVGDPR AGGGDGGSGG GTGPVVQPDH
841 VSVPGNLNSE MGCAGDWDPA CDRAQLTRNP NDDIWRLTTT IPAGDYEYKA AINKGWDENY
901 GLGGARNGSN IPLTATGAPV TFYYDHRTHW VTSDAQGEII TAPGSFQSEL GCAGDWAPDC
961 MRSWLQDPDG DGTYTWSSSQ VPAGSYEFKV THGLSWKVNY GAGGEQNGPN VAFSVPSDGT
1021 LVTISYVLAT HQISVRTSRA GAAPDLTKAK AFIVDDDLVA WPASSVPAGV DPATLTWRLH
1081 WSAGGGLAVD AEAVTGGSVA TLTRDPKGLP AAVLAAHPEL KDAVALRTDK RTAALMPEIL
1141 TGQVAVGMYD STGVLLDATG VQIAIALDAM YSGAARKRSY GVGFPKGGVS FALWAPTAQD
1201 VDLLTWPAGS DDLGVDRARR TPMKRAADGS WAVTLGASAK EARYLYEVRV FAPTTGKVET
1261 NRVTDPSSVA LTLNSTHSVA VDLKDREHMP SAWRTTPSPQ LAQNVDTTIY ELHVRDFSAT
1321 DETVPEELRG SYLAFAQEGD GRKHLRALAE AGLNTVHLLP TFDIASIEED PARQQEPDCD
1381 LESFAPDSEE QQKCTTKVAG ADAFNWGYDP YHWTTPEGSY ASTAAAADGG ARIAEFRTMV
1441 GALHQDGLRV VLDEVYNHTA ESGQGARSVL DKVVPGYYHR LNATGAVETS TCCQNVATEH
1501 AMAEKMMVDS VVTWARDYKV DGFRFDLMGH HSKANLLAIR TALDALTPAR DGVDGTRIYL
1561 YGEGWNFGEV AGNTLFEQAT QGQLGGTHIG TFSDRLRDAV RGGGPFDEDP RKQGFGTGLA
1621 TDPNGAATNG DAAAAAARLA HETDLVQLGL AGNLRTYAFR SQATGEVVQG TDVDYNGSPA
1681 GYADEPDEVV TYVDAHDNET LFDALTYKLP VPTTMADRVR MNSLSLATTA LAQTPSFWHA
1741 GADLLRSKSL NRDSYDSGDW FNELDWTGQD NGFGHGLPPE GVNAAKWPFM RPLLADPALK
1801 PTQDDARQAT AMAQDLLELR FSTPLFRLGS AEAIRAKVSF PVSGTSDAHP GVIVMRIDDT
1861 VGTDVDPALR GLVVVLNASP DAVAQRVPGL SGPVSLSPVQ REGADSVARS AAYDSSSSTL
1921 TVPARTVAVF VQPS
//