LOCUS ADU49215.1 1934 aa PRT BCT 08-JAN-2015 DEFINITION Intrasporangium calvum DSM 43043 alpha-1,6-glucosidase, pullulanase-type protein. ACCESSION CP002343-2653 PROTEIN_ID ADU49215.1 SOURCE Intrasporangium calvum DSM 43043 ORGANISM Intrasporangium calvum DSM 43043 Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae; Intrasporangium. REFERENCE 1 (bases 1 to 4024382) AUTHORS Del Rio,T.G., Chertkov,O., Yasawong,M., Lucas,S., Deshpande,S., Cheng,J.F., Detter,C., Tapia,R., Han,C., Goodwin,L., Pitluck,S., Liolios,K., Ivanova,N., Mavromatis,K., Pati,A., Chen,A., Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D., Rohde,M., Pukall,R., Sikorski,J., Goker,M., Woyke,T., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P. and Lapidus,A. TITLE Complete genome sequence of Intrasporangium calvum type strain (7 KIP) JOURNAL Stand Genomic Sci 3 (3), 294-303 (2010) PUBMED 21304734 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 4024382) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Tapia,R., Detter,J.C., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Intrasporangium calvum DSM 43043 JOURNAL Unpublished REFERENCE 3 (bases 1 to 4024382) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Tapia,R., Han,C., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (17-NOV-2010) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4083316 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Intrasporangium calvum 7KIP, DSM 43043 collection_date :: 1967 lat_lon :: 55.753 37.604 depth :: Missing alt_elev :: Missing country :: Russia environment :: Air num_replicons :: Missing ref_biomaterial :: DSM 43043, ATCC 23552, CUB 430, IFO 12989, IMET 7816, JCM 3097, KCC A-0097, NBRC 12989, NCIB 10167 biotic_relationship :: Free living trophic_level :: Chemoorganotroph rel_to_oxygen :: Aerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi02320 Type Strain :: Yes Greengenes ID :: 90541 Funding Program :: DOE-GEBA 2007 Number of Reads :: 847906 pyrosequencing, 11758818 Illumina Gene Calling Method :: Prodigal 1.4, GenePRIMP Isolation Site :: Air in a school dining room Isolation Comments :: isolated by L.V. Kalakoutskii Motility :: Nonmotile Sporulation :: Nonsporulating Temperature Range :: Mesophile Temperature Optimum :: 37C Gram Staining :: Gram+ Diseases :: None ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454-FLX, 454-Titanium, Illumina GAii ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Intrasporangium calvum DSM 43043" /mol_type="genomic DNA" /strain="DSM 43043" /isolation_source="air in a school dining room" /culture_collection="DSM:43043" /type_material="type strain of Intrasporangium calvum" /db_xref="taxon:710696" /country="Russia" /lat_lon="55.753 N 37.604 E" /collection_date="1967" /collected_by="L.V. Kalakoutskii" protein /locus_tag="Intca_2714" /note="COGs: COG1523 Type II secretory pathway pullulanase PulA and related glycosidase; InterPro IPR006589: IPR011839: IPR006047: IPR004193; KEGG: kfl:Kfla_2775 alpha-1,6-glucosidase, pullulanase-type; PFAM: alpha amylase catalytic region; glycoside hydrolase family 13 domain protein; SMART: alpha amylase catalytic sub domain; SPTR: Alpha-1,6-glucosidase, pullulanase-type; TIGRFAM: alpha-1,6-glucosidase, pullulanase-type; PFAM: Domain of unknown function (DUF3372); Alpha amylase, catalytic domain; Carbohydrate-binding module 48 (Isoamylase N-terminal domain); TIGRFAM: alpha-1,6-glucosidases, pullulanase-type" /transl_table=11 BEGIN 1 MRRRPFLSAA TVAAVAAAGL VTPVSGAVTA WAGDRTVTLV GSLQDELGCP GDWDPGCSAT 61 ELVLEAGSAA YAKVFDVPKG SYELKVAINR SWDENYGADG AKGGANLPLV LEGPARIRFS 121 YDDVSHQIGI APVELSGPAT DADAALATPS LRQAPTKERF YFVMADRFAN GDPSNDDGGL 181 AGDRLATGYD PTDKGFYHGG DLAGISDRLD YIKGLGTTAI WLTPSFKNRP VQGSGDDVSA 241 GYHGYWITDF TQIDPHLGTN AEMTALIDAA HAKGMKVYFD IITNHTADVI DYEGDSDDYR 301 SKEAHPYQDA NGTVFDDTDV AGTSDFPTLD PATSFPYVPT FRTPQDETVK VPAWLNDPVR 361 YHNRGNSTFA GESSLYGDFF GLDDLFTEDP VVVKGMEDIY EAWVDLGIDG FRIDTVKHVN 421 LPFWRSFGPA LMEHAKAAGN DDFFMFGEVF DGSPAYMSRY TTEGRLPATL DFGFQGAALG 481 FASGTPTTGL RDLFAGDDYY TDLDSNAYSL PTFLGNHDMG RVGAMLRDAE FGGDELLART 541 ELANSLMFLT RGQPVVYYGD EQGFTGAGGD KDARQDMFAT QVADYEDDVI IDGGADEPMG 601 SADRYGTAGP LYTHTARLAA LRQAHPALAD GAQLHRYASD SAGIYAVSRI DKNARREYVV 661 VANNATEAKT ATFATASPKT SFLPLLGTSK GVTSDRAGRV TVTVPPLSVQ VFRAKTAFHA 721 PESAITVHPA APAAGGVVGG RAEIGAAMPG NAFAEVTFLQ RPVGTTAWTP IGTDDNAPYR 781 VFHDVTGLAK GTLLEYRMVA RDIEGNLAAT STYGIVGDPR AGGGDGGSGG GTGPVVQPDH 841 VSVPGNLNSE MGCAGDWDPA CDRAQLTRNP NDDIWRLTTT IPAGDYEYKA AINKGWDENY 901 GLGGARNGSN IPLTATGAPV TFYYDHRTHW VTSDAQGEII TAPGSFQSEL GCAGDWAPDC 961 MRSWLQDPDG DGTYTWSSSQ VPAGSYEFKV THGLSWKVNY GAGGEQNGPN VAFSVPSDGT 1021 LVTISYVLAT HQISVRTSRA GAAPDLTKAK AFIVDDDLVA WPASSVPAGV DPATLTWRLH 1081 WSAGGGLAVD AEAVTGGSVA TLTRDPKGLP AAVLAAHPEL KDAVALRTDK RTAALMPEIL 1141 TGQVAVGMYD STGVLLDATG VQIAIALDAM YSGAARKRSY GVGFPKGGVS FALWAPTAQD 1201 VDLLTWPAGS DDLGVDRARR TPMKRAADGS WAVTLGASAK EARYLYEVRV FAPTTGKVET 1261 NRVTDPSSVA LTLNSTHSVA VDLKDREHMP SAWRTTPSPQ LAQNVDTTIY ELHVRDFSAT 1321 DETVPEELRG SYLAFAQEGD GRKHLRALAE AGLNTVHLLP TFDIASIEED PARQQEPDCD 1381 LESFAPDSEE QQKCTTKVAG ADAFNWGYDP YHWTTPEGSY ASTAAAADGG ARIAEFRTMV 1441 GALHQDGLRV VLDEVYNHTA ESGQGARSVL DKVVPGYYHR LNATGAVETS TCCQNVATEH 1501 AMAEKMMVDS VVTWARDYKV DGFRFDLMGH HSKANLLAIR TALDALTPAR DGVDGTRIYL 1561 YGEGWNFGEV AGNTLFEQAT QGQLGGTHIG TFSDRLRDAV RGGGPFDEDP RKQGFGTGLA 1621 TDPNGAATNG DAAAAAARLA HETDLVQLGL AGNLRTYAFR SQATGEVVQG TDVDYNGSPA 1681 GYADEPDEVV TYVDAHDNET LFDALTYKLP VPTTMADRVR MNSLSLATTA LAQTPSFWHA 1741 GADLLRSKSL NRDSYDSGDW FNELDWTGQD NGFGHGLPPE GVNAAKWPFM RPLLADPALK 1801 PTQDDARQAT AMAQDLLELR FSTPLFRLGS AEAIRAKVSF PVSGTSDAHP GVIVMRIDDT 1861 VGTDVDPALR GLVVVLNASP DAVAQRVPGL SGPVSLSPVQ REGADSVARS AAYDSSSSTL 1921 TVPARTVAVF VQPS //