LOCUS ACM56510.1 1567 aa PRT BCT 01-MAR-2021
DEFINITION Halorubrum lacusprofundi ATCC 49239 ferredoxin-dependent
glutamate synthase protein.
ACCESSION CP001365-904
PROTEIN_ID ACM56510.1
SOURCE Halorubrum lacusprofundi ATCC 49239
ORGANISM Halorubrum lacusprofundi ATCC 49239
Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria;
Haloferacales; Haloferacaceae; Halorubrum.
REFERENCE 1 (bases 1 to 2735295)
AUTHORS Dassarma,S., Capes,M.D., Karan,R. and Dassarma,P.
TITLE Amino acid substitutions in cold-adapted proteins from Halorubrum
lacusprofundi, an extremely halophilic microbe from antarctica
JOURNAL PLoS One 8 (3), e58587 (2013)
PUBMED 23536799
REFERENCE 2 (bases 1 to 2735295)
AUTHORS Karan,R., Capes,M.D., DasSarma,P. and DasSarma,S.
TITLE Cloning, overexpression, purification, and characterization of a
polyextremophilic beta-galactosidase from the Antarctic
haloarchaeon Halorubrum lacusprofundi
JOURNAL BMC Biotechnol 13, 3 (2013)
PUBMED 23320757
REMARK Publication Status: Online-Only
REFERENCE 3 (bases 1 to 2735295)
AUTHORS Anderson,I.J., DasSarma,P., Lucas,S., Copeland,A., Lapidus,A., Del
Rio,T.G., Tice,H., Dalin,E., Bruce,D.C., Goodwin,L., Pitluck,S.,
Sims,D., Brettin,T.S., Detter,J.C., Han,C.S., Larimer,F.,
Hauser,L., Land,M., Ivanova,N., Richardson,P., Cavicchioli,R.,
DasSarma,S., Woese,C.R. and Kyrpides,N.C.
TITLE Complete genome sequence of the Antarctic Halorubrum lacusprofundi
type strain ACAM 34
JOURNAL Stand Genomic Sci 11 (1), 70 (2016)
PUBMED 27617060
REMARK Publication Status: Online-Only
REFERENCE 4 (bases 1 to 2735295)
AUTHORS Laye,V.J., Karan,R., Kim,J.M., Pecher,W.T., DasSarma,P. and
DasSarma,S.
TITLE Key amino acid residues conferring enhanced enzyme activity at cold
temperatures in an Antarctic polyextremophilic beta-galactosidase
JOURNAL Proc Natl Acad Sci U S A 114 (47), 12530-12535 (2017)
PUBMED 29109294
REFERENCE 5 (bases 1 to 2735295)
AUTHORS Laye,V.J. and DasSarma,S.
TITLE An Antarctic Extreme Halophile and Its Polyextremophilic Enzyme:
Effects of Perchlorate Salts
JOURNAL Astrobiology 18 (4), 412-418 (2018)
PUBMED 29189043
REFERENCE 6 (bases 1 to 2735295)
AUTHORS DasSarma,S., DasSarma,P., Laye,V.J. and Schwieterman,E.W.
TITLE Extremophilic models for astrobiology: haloarchaeal survival
strategies and pigments for remote sensing
JOURNAL Extremophiles 24 (1), 31-41 (2020)
PUBMED 31463573
REFERENCE 7 (bases 1 to 2735295)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T.,
Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and
Richardson,P.
CONSRTM US DOE Joint Genome Institute
TITLE Direct Submission
JOURNAL Submitted (14-JAN-2009) US DOE Joint Genome Institute, 2800
Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4001421
Source DNA available from Professor Shiladitya DasSarma
Archaea available from ATCC: ATCC 49239
Contacts: Ricardo Cavicchioli (r.cavicchioli@unsw.edu.au)
Shiladitya DasSarma (sdassarma@som.umaryland.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Annotation done by JGI-ORNL and JGI-PGF
Finishing done by JGI-LANL
Finished microbial genomes have been curated to close all gaps with
greater than 98% coverage of at least two independent clones. Each
base pair has a minimum q (quality) value of 30 and the total error
rate is less than one per 50000.
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. it is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
FEATURES Qualifiers
source /organism="Halorubrum lacusprofundi ATCC 49239"
/mol_type="genomic DNA"
/strain="ATCC 49239"
/culture_collection="ATCC:49239"
/type_material="type strain of Halorubrum lacusprofundi"
/db_xref="taxon:416348"
/chromosome="1"
protein /locus_tag="Hlac_0912"
/EC_number="1.4.7.1"
/inference="protein motif:PFAM:PF01645"
/note="PFAM: glutamine amidotransferase class-II;
glutamate synthase alpha subunit domain protein;
ferredoxin-dependent glutamate synthase; glutamate
synthase; KEGG: hwa:HQ1714A glutamate synthase large
subunit"
/transl_table=11
/db_xref="InterPro:IPR000583"
/db_xref="InterPro:IPR002489"
/db_xref="InterPro:IPR002932"
/db_xref="InterPro:IPR006982"
BEGIN
1 MTEPRSNTRE VSERDLSGHG GGLAAVTDER SNCGIGGIVD LDGDPSHGTV ADAVELLENL
61 EHRGTTGAEE NTGDGAGIMV ARPDDFFREV VDADLPEVYA VGSVFMPTEP SVQAEIHDVI
121 AEVFSVYGLE VLEWRSVPTD NADLGATALD SEPEVAQLFV APVEGAGLAE RFTSEETRPD
181 DADPEILGFD RALYAARREI ENAVSDIDGA GRFYVCSLDR QRVVYKGLLK GSQLAGYYPD
241 LTDERFASHV ALVHARFSTN TLGAWHLAHP YRNIVHNGEF NTIRGNVNWM RARENDLDHP
301 AFGAELDTIK PVIDDPNQSD TASVDNAVEL LLQAGRDLPH TLRMLIPEAF RDDDLMEQAR
361 ADFYDYHASL VEPWDGPALV IGFDGDRVAG VLDRNGLRPC RYEVTEDNRL VVGSEVGALP
421 TEPADVRRRG RLQPGEIFVA DPEQGRIVPD DEVFDDLTDE KYGEWVAERQ VALDEIVEED
481 DVAAGVESES DDSDDTGEDG DAEPTVRAHQ AAFGYTTDSL DHLVEPMARD GKDPVGSMGD
541 DTPLSVLADV DRPLFTYFKQ LFAQVSNPPI DYIREELVTS LETRIGNQRN LLDETPEHAE
601 QIVSDTPVLT DAETAALREL PGPGEREPAD RKGDEPDASS FTSVVVDVTY DRDGSLVDAV
661 NRIREDAATA IEDGADAVVL SDRAVGTDRL AVPSLLATGA AHHHLVRNGL RNRAGLVVES
721 GEPHEVHHLA TLVGYGADAV DPYLAYASIA DVVAGPDGAD EDEALAAYRH ALEDGLLKTM
781 AKMGISTMES YQGAQIFEAV GLSSSFVAEY FEGTEIRTEG IGIGEIEDDL RTRHAMAFGA
841 DPELETTGEY EHRSSGIKHG WNPQTVGTLQ QAVRGGDYEQ YQEFAELVND ESEKLQTLRG
901 LLEFESDRDA VPVEEVEPVE SIVERFSTAA MSLGSISPEA HENNAIAMNR LGAKSNTGEG
961 GEPPERFGTE KECNVKQVAS GRFGVTSEYL ASADELQIKM AQGSKPGEGG HLPGKKVNEM
1021 IAHVRCSTPG VGLISPPPQH DIYSIEDLKQ LIFDLKAASP DADVNVKLVS EAGIGTIAAG
1081 VAKANADVVH VSGHDGGTGA SPKTSIKNAG LPWELGLAEA NQMLRATGLR DRIRVTADGG
1141 LKTGRDVAVA AALGAEEYVF GTASLVTAGC VMARQCHENT CPVGVATQRE DLRQRFPGQP
1201 DHVINYMTFI AQELRELMAD LGYTEVEEFI GRPELLSQRE TDHEKAKHLD LSAVIAEPAG
1261 DARTKTREQD DADIDDLLDW DLIEEASPAI EDGAPVALTR DVENVDRAIG ATLSNRIVSE
1321 HGGDGLPDDT VTCRFDGYAG QSFGAFLAPG ITMELSGAAN DYVGKGLSGG RIVINTPEEA
1381 GYDPAENVVA GNVGLYGATA GEVYVNGVAG ERFGVRNSGV KAVVEGVGDH GCEYMTGGAV
1441 AVLGDTGRNF AAGMSGGVAY VYDPDDRLDA RVNRGMVSLS ETLDESDERM LRRLVENHRA
1501 YTDSDRAAEL LDDWAAALDD FVRVFPDAYA EVIAEGTGAD VREELPEPAA AVPGSASDAT
1561 EQASSDD
//