LOCUS ACM56510.1 1567 aa PRT BCT 01-MAR-2021 DEFINITION Halorubrum lacusprofundi ATCC 49239 ferredoxin-dependent glutamate synthase protein. ACCESSION CP001365-904 PROTEIN_ID ACM56510.1 SOURCE Halorubrum lacusprofundi ATCC 49239 ORGANISM Halorubrum lacusprofundi ATCC 49239 Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Halorubrum. REFERENCE 1 (bases 1 to 2735295) AUTHORS Dassarma,S., Capes,M.D., Karan,R. and Dassarma,P. TITLE Amino acid substitutions in cold-adapted proteins from Halorubrum lacusprofundi, an extremely halophilic microbe from antarctica JOURNAL PLoS One 8 (3), e58587 (2013) PUBMED 23536799 REFERENCE 2 (bases 1 to 2735295) AUTHORS Karan,R., Capes,M.D., DasSarma,P. and DasSarma,S. TITLE Cloning, overexpression, purification, and characterization of a polyextremophilic beta-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi JOURNAL BMC Biotechnol 13, 3 (2013) PUBMED 23320757 REMARK Publication Status: Online-Only REFERENCE 3 (bases 1 to 2735295) AUTHORS Anderson,I.J., DasSarma,P., Lucas,S., Copeland,A., Lapidus,A., Del Rio,T.G., Tice,H., Dalin,E., Bruce,D.C., Goodwin,L., Pitluck,S., Sims,D., Brettin,T.S., Detter,J.C., Han,C.S., Larimer,F., Hauser,L., Land,M., Ivanova,N., Richardson,P., Cavicchioli,R., DasSarma,S., Woese,C.R. and Kyrpides,N.C. TITLE Complete genome sequence of the Antarctic Halorubrum lacusprofundi type strain ACAM 34 JOURNAL Stand Genomic Sci 11 (1), 70 (2016) PUBMED 27617060 REMARK Publication Status: Online-Only REFERENCE 4 (bases 1 to 2735295) AUTHORS Laye,V.J., Karan,R., Kim,J.M., Pecher,W.T., DasSarma,P. and DasSarma,S. TITLE Key amino acid residues conferring enhanced enzyme activity at cold temperatures in an Antarctic polyextremophilic beta-galactosidase JOURNAL Proc Natl Acad Sci U S A 114 (47), 12530-12535 (2017) PUBMED 29109294 REFERENCE 5 (bases 1 to 2735295) AUTHORS Laye,V.J. and DasSarma,S. TITLE An Antarctic Extreme Halophile and Its Polyextremophilic Enzyme: Effects of Perchlorate Salts JOURNAL Astrobiology 18 (4), 412-418 (2018) PUBMED 29189043 REFERENCE 6 (bases 1 to 2735295) AUTHORS DasSarma,S., DasSarma,P., Laye,V.J. and Schwieterman,E.W. TITLE Extremophilic models for astrobiology: haloarchaeal survival strategies and pigments for remote sensing JOURNAL Extremophiles 24 (1), 31-41 (2020) PUBMED 31463573 REFERENCE 7 (bases 1 to 2735295) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (14-JAN-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4001421 Source DNA available from Professor Shiladitya DasSarma Archaea available from ATCC: ATCC 49239 Contacts: Ricardo Cavicchioli (r.cavicchioli@unsw.edu.au) Shiladitya DasSarma (sdassarma@som.umaryland.edu) David Bruce (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of at least two independent clones. Each base pair has a minimum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). FEATURES Qualifiers source /organism="Halorubrum lacusprofundi ATCC 49239" /mol_type="genomic DNA" /strain="ATCC 49239" /culture_collection="ATCC:49239" /type_material="type strain of Halorubrum lacusprofundi" /db_xref="taxon:416348" /chromosome="1" protein /locus_tag="Hlac_0912" /EC_number="1.4.7.1" /inference="protein motif:PFAM:PF01645" /note="PFAM: glutamine amidotransferase class-II; glutamate synthase alpha subunit domain protein; ferredoxin-dependent glutamate synthase; glutamate synthase; KEGG: hwa:HQ1714A glutamate synthase large subunit" /transl_table=11 /db_xref="InterPro:IPR000583" /db_xref="InterPro:IPR002489" /db_xref="InterPro:IPR002932" /db_xref="InterPro:IPR006982" BEGIN 1 MTEPRSNTRE VSERDLSGHG GGLAAVTDER SNCGIGGIVD LDGDPSHGTV ADAVELLENL 61 EHRGTTGAEE NTGDGAGIMV ARPDDFFREV VDADLPEVYA VGSVFMPTEP SVQAEIHDVI 121 AEVFSVYGLE VLEWRSVPTD NADLGATALD SEPEVAQLFV APVEGAGLAE RFTSEETRPD 181 DADPEILGFD RALYAARREI ENAVSDIDGA GRFYVCSLDR QRVVYKGLLK GSQLAGYYPD 241 LTDERFASHV ALVHARFSTN TLGAWHLAHP YRNIVHNGEF NTIRGNVNWM RARENDLDHP 301 AFGAELDTIK PVIDDPNQSD TASVDNAVEL LLQAGRDLPH TLRMLIPEAF RDDDLMEQAR 361 ADFYDYHASL VEPWDGPALV IGFDGDRVAG VLDRNGLRPC RYEVTEDNRL VVGSEVGALP 421 TEPADVRRRG RLQPGEIFVA DPEQGRIVPD DEVFDDLTDE KYGEWVAERQ VALDEIVEED 481 DVAAGVESES DDSDDTGEDG DAEPTVRAHQ AAFGYTTDSL DHLVEPMARD GKDPVGSMGD 541 DTPLSVLADV DRPLFTYFKQ LFAQVSNPPI DYIREELVTS LETRIGNQRN LLDETPEHAE 601 QIVSDTPVLT DAETAALREL PGPGEREPAD RKGDEPDASS FTSVVVDVTY DRDGSLVDAV 661 NRIREDAATA IEDGADAVVL SDRAVGTDRL AVPSLLATGA AHHHLVRNGL RNRAGLVVES 721 GEPHEVHHLA TLVGYGADAV DPYLAYASIA DVVAGPDGAD EDEALAAYRH ALEDGLLKTM 781 AKMGISTMES YQGAQIFEAV GLSSSFVAEY FEGTEIRTEG IGIGEIEDDL RTRHAMAFGA 841 DPELETTGEY EHRSSGIKHG WNPQTVGTLQ QAVRGGDYEQ YQEFAELVND ESEKLQTLRG 901 LLEFESDRDA VPVEEVEPVE SIVERFSTAA MSLGSISPEA HENNAIAMNR LGAKSNTGEG 961 GEPPERFGTE KECNVKQVAS GRFGVTSEYL ASADELQIKM AQGSKPGEGG HLPGKKVNEM 1021 IAHVRCSTPG VGLISPPPQH DIYSIEDLKQ LIFDLKAASP DADVNVKLVS EAGIGTIAAG 1081 VAKANADVVH VSGHDGGTGA SPKTSIKNAG LPWELGLAEA NQMLRATGLR DRIRVTADGG 1141 LKTGRDVAVA AALGAEEYVF GTASLVTAGC VMARQCHENT CPVGVATQRE DLRQRFPGQP 1201 DHVINYMTFI AQELRELMAD LGYTEVEEFI GRPELLSQRE TDHEKAKHLD LSAVIAEPAG 1261 DARTKTREQD DADIDDLLDW DLIEEASPAI EDGAPVALTR DVENVDRAIG ATLSNRIVSE 1321 HGGDGLPDDT VTCRFDGYAG QSFGAFLAPG ITMELSGAAN DYVGKGLSGG RIVINTPEEA 1381 GYDPAENVVA GNVGLYGATA GEVYVNGVAG ERFGVRNSGV KAVVEGVGDH GCEYMTGGAV 1441 AVLGDTGRNF AAGMSGGVAY VYDPDDRLDA RVNRGMVSLS ETLDESDERM LRRLVENHRA 1501 YTDSDRAAEL LDDWAAALDD FVRVFPDAYA EVIAEGTGAD VREELPEPAA AVPGSASDAT 1561 EQASSDD //