LOCUS CK335234 680 bp mRNA linear EST 12-MAY-2010
DEFINITION H3154E05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3154E05 5', mRNA sequence.
ACCESSION CK335234
VERSION CK335234.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 680)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3154 row: E column: 05
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..680
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3154E05-5"
/db_xref="taxon:10090"
/clone="H3154E05"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 196 a 132 c 181 g 171 t
ORIGIN
1 agcatcctag cggttggact caaacgcaga ggcccgggac tgacgccggt tgaacgggtc
61 tggagacggc gcttggacga agcggcccgg ccagactgat caaaaggatg gattttacag
121 aggcttactc agacacgtgt tctacggttg gacttgctgc cagagaaggc aatgctaaaa
181 ttctaaggaa attgctcaaa aaaggccgaa gtgtggatgt cgctgataac aggggatgga
241 tgccgattca tgaagcagct tatcacaatg ctgtagagtg tttgcagatg ctaattcata
301 cagattcatc tgaaaactac attaaggcaa agacgtttga gggcttctgt gcattgcatc
361 ttgctgtgag tcaaggacat tggaaaatca cacagattct tttggaagct ggagctgatc
421 ctaatgaaac cactttagaa gaaactacac cattgtttta gctgttgaaa gtggacggat
481 agatgtatta aagctgttgc ttcaacatgg agcaaatgtc aatggatccc attctatgag
541 tggatggaat tccttgcacc aggcttcttt ccagggaaat gctgagacta taagattgct
601 tcttaaacaa ggagcagaca gagaatgcca agatgacttt ggaatcacac ctctgtttgt
661 ggctgctcag tatggggagc
//