LOCUS CK335228 669 bp mRNA linear EST 12-MAY-2010
DEFINITION H3154B01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3154B01 5', mRNA sequence.
ACCESSION CK335228
VERSION CK335228.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 669)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3154 row: B column: 01
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..669
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3154B01-5"
/db_xref="taxon:10090"
/clone="H3154B01"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 182 a 146 c 138 g 203 t
ORIGIN
1 tctaaatcag gccaggcatg gtggttcaca tctttttaat acaagtactt caggatgcaa
61 aggcaagtgg atctctgaga ctagcctagt ctacaaagag agtctaggat agccaagtct
121 ctgttgcaca gagaccctgc ctcagagaaa agagtaaatg tctattttat cttttgaaga
181 aagttaaaca agattaatga atttactggg gaaatactgg tggtcttaca gattatttgg
241 tggggccttt aacaaagagt aacttctatc tgctagtggc tggtcccacc ttcactcttc
301 cttcccagaa tgcttgcaat cttttctgtt aaacccaatt ttaaaataga aaatactttt
361 gtccctgaaa caggcctatc cctccttcac tttacttatg gcagttacat atgtgctcgg
421 tcttaggtgc actatccttt accatactac ttacaataag ctattgtgct atgttcccat
481 aatgtctttg tacttacaag tgttttaata gctatcatct ttaatagtgt atatctgagc
541 cgggcagtgg tggcgtatgc ctttactccc agcacttggg acacagaggc aggctgatcc
601 ctgagttcga ggccagcctg gtctacagag tgagttccag gacatccagg gctacacaga
661 gaaaccctg
//